Abnormal ovarian DNA methylation programming during gonad maturation in wild contaminated fish
[Departement_IRSTEA]Eaux [TR1_IRSTEA]QUASARE International audience There is increasing evidence that pollutants may cause diseases via epigenetic modifications. Epigenetic mechanisms such as DNA methylation participate in the regulation of gene transcription. Surprisingly, epigenetics research is s...
Published in: | Environmental Science & Technology |
---|---|
Main Authors: | , , , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2014
|
Subjects: | |
Online Access: | https://hal.science/hal-02289133 https://doi.org/10.1021/es503712c |
id |
ftunivrennes2hal:oai:HAL:hal-02289133v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Archive Ouverte de l'Université Rennes (HAL) |
op_collection_id |
ftunivrennes2hal |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Pierron, F. Bureau Du Colombier, S. Moffett, A. Caron, A. Peluhet, L. Daffe, G. Lambert, Patrick Elie, Pierre Labadie, P. Budzinski, H. Dufour, S. Couture, P. Baudrimont, M. Abnormal ovarian DNA methylation programming during gonad maturation in wild contaminated fish |
topic_facet |
[SDE]Environmental Sciences |
description |
[Departement_IRSTEA]Eaux [TR1_IRSTEA]QUASARE International audience There is increasing evidence that pollutants may cause diseases via epigenetic modifications. Epigenetic mechanisms such as DNA methylation participate in the regulation of gene transcription. Surprisingly, epigenetics research is still limited in ecotoxicology. In this study, we investigated whether chronic exposure to contaminants experienced by wild female fish (Anguilla anguilla) throughout their juvenile phase can affect the DNA methylation status of their oocytes during gonad maturation. Thus, fish were sampled in two locations presenting a low or a high contamination level. Then, fish were transferred to the laboratory and artificially matured. Before hormonal treatment, the DNA methylation levels of the genes encoding for the aromatase and the receptor of the follicle stimulating hormone were higher in contaminated fish than in fish from the clean site. For the hormone receptor, this hypermethylation was positively correlated with the contamination level of fish and was associated with a decrease in its transcription level. In addition, whereas gonad growth was associated with an increase in DNA methylation in fish from the clean site, no changes were observed in contaminated fish in response to hormonal treatment. Finally, a higher gonad growth was observed in fish from the reference site in comparison to contaminated fish. |
author2 |
Faculty of Engineering and the Environment (µVIS X-ray Imaging Centre) University of Southampton Laboratoire de Physico et Toxico-Chimie des systèmes naturels (LPTC) Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS) Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Ecosystèmes aquatiques et changements globaux (UR EABX) Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) Institut des Sciences Moléculaires (ISM) Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Littoral, Environnement, Télédétection, Géomatique (LETG - Rennes) Littoral, Environnement, Télédétection, Géomatique UMR 6554 (LETG) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Université d'Angers (UA)-École Pratique des Hautes Études (EPHE) Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut de Géographie et d'Aménagement Régional de l'Université de Nantes (IGARUN) Université de Nantes (UN)-Université de Nantes (UN)-Université de Caen Normandie (UNICAEN) Université de Nantes (UN)-Université de Nantes (UN) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Institut National de la Recherche Scientifique Québec (INRS) |
format |
Article in Journal/Newspaper |
author |
Pierron, F. Bureau Du Colombier, S. Moffett, A. Caron, A. Peluhet, L. Daffe, G. Lambert, Patrick Elie, Pierre Labadie, P. Budzinski, H. Dufour, S. Couture, P. Baudrimont, M. |
author_facet |
Pierron, F. Bureau Du Colombier, S. Moffett, A. Caron, A. Peluhet, L. Daffe, G. Lambert, Patrick Elie, Pierre Labadie, P. Budzinski, H. Dufour, S. Couture, P. Baudrimont, M. |
author_sort |
Pierron, F. |
title |
Abnormal ovarian DNA methylation programming during gonad maturation in wild contaminated fish |
title_short |
Abnormal ovarian DNA methylation programming during gonad maturation in wild contaminated fish |
title_full |
Abnormal ovarian DNA methylation programming during gonad maturation in wild contaminated fish |
title_fullStr |
Abnormal ovarian DNA methylation programming during gonad maturation in wild contaminated fish |
title_full_unstemmed |
Abnormal ovarian DNA methylation programming during gonad maturation in wild contaminated fish |
title_sort |
abnormal ovarian dna methylation programming during gonad maturation in wild contaminated fish |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.science/hal-02289133 https://doi.org/10.1021/es503712c |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
ISSN: 0013-936X EISSN: 1520-5851 Environmental Science and Technology https://hal.science/hal-02289133 Environmental Science and Technology, 2014, 48 (19), pp.11688--11695. ⟨10.1021/es503712c⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1021/es503712c hal-02289133 https://hal.science/hal-02289133 doi:10.1021/es503712c IRSTEA: PUB00042032 |
op_doi |
https://doi.org/10.1021/es503712c |
container_title |
Environmental Science & Technology |
container_volume |
48 |
container_issue |
19 |
container_start_page |
11688 |
op_container_end_page |
11695 |
_version_ |
1809941215388368896 |
spelling |
ftunivrennes2hal:oai:HAL:hal-02289133v1 2024-09-09T19:00:40+00:00 Abnormal ovarian DNA methylation programming during gonad maturation in wild contaminated fish Pierron, F. Bureau Du Colombier, S. Moffett, A. Caron, A. Peluhet, L. Daffe, G. Lambert, Patrick Elie, Pierre Labadie, P. Budzinski, H. Dufour, S. Couture, P. Baudrimont, M. Faculty of Engineering and the Environment (µVIS X-ray Imaging Centre) University of Southampton Laboratoire de Physico et Toxico-Chimie des systèmes naturels (LPTC) Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS) Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Ecosystèmes aquatiques et changements globaux (UR EABX) Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) Institut des Sciences Moléculaires (ISM) Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Littoral, Environnement, Télédétection, Géomatique (LETG - Rennes) Littoral, Environnement, Télédétection, Géomatique UMR 6554 (LETG) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Université d'Angers (UA)-École Pratique des Hautes Études (EPHE) Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut de Géographie et d'Aménagement Régional de l'Université de Nantes (IGARUN) Université de Nantes (UN)-Université de Nantes (UN)-Université de Caen Normandie (UNICAEN) Université de Nantes (UN)-Université de Nantes (UN) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Institut National de la Recherche Scientifique Québec (INRS) 2014 https://hal.science/hal-02289133 https://doi.org/10.1021/es503712c en eng HAL CCSD American Chemical Society info:eu-repo/semantics/altIdentifier/doi/10.1021/es503712c hal-02289133 https://hal.science/hal-02289133 doi:10.1021/es503712c IRSTEA: PUB00042032 ISSN: 0013-936X EISSN: 1520-5851 Environmental Science and Technology https://hal.science/hal-02289133 Environmental Science and Technology, 2014, 48 (19), pp.11688--11695. ⟨10.1021/es503712c⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2014 ftunivrennes2hal https://doi.org/10.1021/es503712c 2024-06-17T14:18:31Z [Departement_IRSTEA]Eaux [TR1_IRSTEA]QUASARE International audience There is increasing evidence that pollutants may cause diseases via epigenetic modifications. Epigenetic mechanisms such as DNA methylation participate in the regulation of gene transcription. Surprisingly, epigenetics research is still limited in ecotoxicology. In this study, we investigated whether chronic exposure to contaminants experienced by wild female fish (Anguilla anguilla) throughout their juvenile phase can affect the DNA methylation status of their oocytes during gonad maturation. Thus, fish were sampled in two locations presenting a low or a high contamination level. Then, fish were transferred to the laboratory and artificially matured. Before hormonal treatment, the DNA methylation levels of the genes encoding for the aromatase and the receptor of the follicle stimulating hormone were higher in contaminated fish than in fish from the clean site. For the hormone receptor, this hypermethylation was positively correlated with the contamination level of fish and was associated with a decrease in its transcription level. In addition, whereas gonad growth was associated with an increase in DNA methylation in fish from the clean site, no changes were observed in contaminated fish in response to hormonal treatment. Finally, a higher gonad growth was observed in fish from the reference site in comparison to contaminated fish. Article in Journal/Newspaper Anguilla anguilla Archive Ouverte de l'Université Rennes (HAL) Environmental Science & Technology 48 19 11688 11695 |