Development of the Analysis of Fecal Stanols in the Oyster Crassostrea gigas and Identification of Fecal Contamination in Shellfish Harvesting Areas
International audience The objective of this work was to study the effects of washing and purification steps on qualitative and quantitative analysis of fecal stanols in the oyster Crassostrea gigas using either single or a combination of lipid purification steps on silica gel or aminopropyl bonded...
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ftunivrennes1hal:oai:HAL:insu-01002609v1 2024-02-11T10:03:08+01:00 Development of the Analysis of Fecal Stanols in the Oyster Crassostrea gigas and Identification of Fecal Contamination in Shellfish Harvesting Areas Harrault, Loïc Jardé, Emilie Jeanneau, Laurent Petitjean, Patrice Géosciences Rennes (GR) Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR) Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS) 2014 https://insu.hal.science/insu-01002609 https://insu.hal.science/insu-01002609/document https://insu.hal.science/insu-01002609/file/Harrault-LIPIDS-2014-HAL.pdf https://doi.org/10.1007/s11745-014-3908-5 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s11745-014-3908-5 insu-01002609 https://insu.hal.science/insu-01002609 https://insu.hal.science/insu-01002609/document https://insu.hal.science/insu-01002609/file/Harrault-LIPIDS-2014-HAL.pdf doi:10.1007/s11745-014-3908-5 info:eu-repo/semantics/OpenAccess ISSN: 0024-4201 EISSN: 1558-9307 Lipids https://insu.hal.science/insu-01002609 Lipids, 2014, 49 (6), pp.597-607. ⟨10.1007/s11745-014-3908-5⟩ Stanol analysis Oysters Mass spectrometry Gas chromatography GEOF Fecal contamination Microbial source tracking [SDU.STU]Sciences of the Universe [physics]/Earth Sciences info:eu-repo/semantics/article Journal articles 2014 ftunivrennes1hal https://doi.org/10.1007/s11745-014-3908-5 2024-01-23T23:56:45Z International audience The objective of this work was to study the effects of washing and purification steps on qualitative and quantitative analysis of fecal stanols in the oyster Crassostrea gigas using either single or a combination of lipid purification steps on silica gel or aminopropyl bonded silica gel (NH2) or a washing step. Among the three analytical pathways compared, the two including water extraction or NH2 purification did not lead to higher recoveries and decreased repeatabilities of extractions compared to the single purification on silica gel. This latter led to similar recoveries (ca. 80 %) and repeatabilities (ca. 10 %) for both spiked standards (coprostanol and sitostanol). This analytical pathway has been applied to oysters collected in a harvesting area in Brittany (France) where fecal contaminations are important and allowed to quantify eight stanols in oysters. The relative proportions of fecal stanols of these oysters were combined with principal component analysis in order to investigate the usefulness of their stanol fingerprints to record a fecal contamination and to distinguish its source between human, porcine and bovine contaminations. Oysters non-fecally contaminated by Escherichia coli did not present specific stanol fingerprints while oysters fecally contaminated had a bovine fingerprint, suggesting a contamination of these samples by bovine sources. As a consequence, the method developed here allows the use of stanol fingerprints of oysters as a microbial source tracking tool that can be applied to shellfish harvesting areas subjected to fecal contaminations in order to identify the different sources of contamination and improve watershed management. Article in Journal/Newspaper Crassostrea gigas Université de Rennes 1: Publications scientifiques (HAL) Lipids 49 6 597 607 |
institution |
Open Polar |
collection |
Université de Rennes 1: Publications scientifiques (HAL) |
op_collection_id |
ftunivrennes1hal |
language |
English |
topic |
Stanol analysis Oysters Mass spectrometry Gas chromatography GEOF Fecal contamination Microbial source tracking [SDU.STU]Sciences of the Universe [physics]/Earth Sciences |
spellingShingle |
Stanol analysis Oysters Mass spectrometry Gas chromatography GEOF Fecal contamination Microbial source tracking [SDU.STU]Sciences of the Universe [physics]/Earth Sciences Harrault, Loïc Jardé, Emilie Jeanneau, Laurent Petitjean, Patrice Development of the Analysis of Fecal Stanols in the Oyster Crassostrea gigas and Identification of Fecal Contamination in Shellfish Harvesting Areas |
topic_facet |
Stanol analysis Oysters Mass spectrometry Gas chromatography GEOF Fecal contamination Microbial source tracking [SDU.STU]Sciences of the Universe [physics]/Earth Sciences |
description |
International audience The objective of this work was to study the effects of washing and purification steps on qualitative and quantitative analysis of fecal stanols in the oyster Crassostrea gigas using either single or a combination of lipid purification steps on silica gel or aminopropyl bonded silica gel (NH2) or a washing step. Among the three analytical pathways compared, the two including water extraction or NH2 purification did not lead to higher recoveries and decreased repeatabilities of extractions compared to the single purification on silica gel. This latter led to similar recoveries (ca. 80 %) and repeatabilities (ca. 10 %) for both spiked standards (coprostanol and sitostanol). This analytical pathway has been applied to oysters collected in a harvesting area in Brittany (France) where fecal contaminations are important and allowed to quantify eight stanols in oysters. The relative proportions of fecal stanols of these oysters were combined with principal component analysis in order to investigate the usefulness of their stanol fingerprints to record a fecal contamination and to distinguish its source between human, porcine and bovine contaminations. Oysters non-fecally contaminated by Escherichia coli did not present specific stanol fingerprints while oysters fecally contaminated had a bovine fingerprint, suggesting a contamination of these samples by bovine sources. As a consequence, the method developed here allows the use of stanol fingerprints of oysters as a microbial source tracking tool that can be applied to shellfish harvesting areas subjected to fecal contaminations in order to identify the different sources of contamination and improve watershed management. |
author2 |
Géosciences Rennes (GR) Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR) Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Harrault, Loïc Jardé, Emilie Jeanneau, Laurent Petitjean, Patrice |
author_facet |
Harrault, Loïc Jardé, Emilie Jeanneau, Laurent Petitjean, Patrice |
author_sort |
Harrault, Loïc |
title |
Development of the Analysis of Fecal Stanols in the Oyster Crassostrea gigas and Identification of Fecal Contamination in Shellfish Harvesting Areas |
title_short |
Development of the Analysis of Fecal Stanols in the Oyster Crassostrea gigas and Identification of Fecal Contamination in Shellfish Harvesting Areas |
title_full |
Development of the Analysis of Fecal Stanols in the Oyster Crassostrea gigas and Identification of Fecal Contamination in Shellfish Harvesting Areas |
title_fullStr |
Development of the Analysis of Fecal Stanols in the Oyster Crassostrea gigas and Identification of Fecal Contamination in Shellfish Harvesting Areas |
title_full_unstemmed |
Development of the Analysis of Fecal Stanols in the Oyster Crassostrea gigas and Identification of Fecal Contamination in Shellfish Harvesting Areas |
title_sort |
development of the analysis of fecal stanols in the oyster crassostrea gigas and identification of fecal contamination in shellfish harvesting areas |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://insu.hal.science/insu-01002609 https://insu.hal.science/insu-01002609/document https://insu.hal.science/insu-01002609/file/Harrault-LIPIDS-2014-HAL.pdf https://doi.org/10.1007/s11745-014-3908-5 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0024-4201 EISSN: 1558-9307 Lipids https://insu.hal.science/insu-01002609 Lipids, 2014, 49 (6), pp.597-607. ⟨10.1007/s11745-014-3908-5⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11745-014-3908-5 insu-01002609 https://insu.hal.science/insu-01002609 https://insu.hal.science/insu-01002609/document https://insu.hal.science/insu-01002609/file/Harrault-LIPIDS-2014-HAL.pdf doi:10.1007/s11745-014-3908-5 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1007/s11745-014-3908-5 |
container_title |
Lipids |
container_volume |
49 |
container_issue |
6 |
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597 |
op_container_end_page |
607 |
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