Chromosomal mapping of canine-derived BAC clones to the red fox and American mink genomes.
International audience High-quality sequencing of the dog (Canis lupus familiaris) genome has enabled enormous progress in genetic mapping of canine phenotypic variation. The red fox (Vulpes vulpes), another canid species, also exhibits a wide range of variation in coat color, morphology, and behavi...
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Online Access: | https://www.hal.inserm.fr/inserm-00405774 https://doi.org/10.1093/jhered/esp037 |
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ftunivrennes1hal:oai:HAL:inserm-00405774v1 2023-08-20T04:05:49+02:00 Chromosomal mapping of canine-derived BAC clones to the red fox and American mink genomes. Kukekova, Anna, Vorobieva, Nadegda, Beklemisheva, Violetta, Johnson, Jennifer, Temnykh, Svetlana, Yudkin, Dmitry, Trut, Lyudmila, André, Catherine Galibert, Francis Aguirre, Gustavo, Acland, Gregory, Graphodatsky, Alexander J. A. Baker Institute Cornell University New York Institute of Cytology and Genetics Russian Academy of Sciences Moscow (RAS) Institut de Génétique et Développement de Rennes (IGDR) Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS) Department of Clinical Studies University of Pennsylvania 2009-06-21 https://www.hal.inserm.fr/inserm-00405774 https://doi.org/10.1093/jhered/esp037 en eng HAL CCSD Oxford University Press (OUP) info:eu-repo/semantics/altIdentifier/doi/10.1093/jhered/esp037 info:eu-repo/semantics/altIdentifier/pmid/19546120 inserm-00405774 https://www.hal.inserm.fr/inserm-00405774 doi:10.1093/jhered/esp037 PUBMED: 19546120 PUBMEDCENTRAL: PMC3139363 ISSN: 0022-1503 EISSN: 1471-8505 Journal of Heredity https://www.hal.inserm.fr/inserm-00405774 Journal of Heredity, 2009, 100 Suppl 1, pp.S42-53. ⟨10.1093/jhered/esp037⟩ [SDV.GEN]Life Sciences [q-bio]/Genetics [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] info:eu-repo/semantics/article Journal articles 2009 ftunivrennes1hal https://doi.org/10.1093/jhered/esp037 2023-08-01T22:44:45Z International audience High-quality sequencing of the dog (Canis lupus familiaris) genome has enabled enormous progress in genetic mapping of canine phenotypic variation. The red fox (Vulpes vulpes), another canid species, also exhibits a wide range of variation in coat color, morphology, and behavior. Although the fox genome has not yet been sequenced, canine genomic resources have been used to construct a meiotic linkage map of the red fox genome and begin genetic mapping in foxes. However, a more detailed gene-specific comparative map between the dog and fox genomes is required to establish gene order within homologous regions of dog and fox chromosomes and to refine breakpoints between homologous chromosomes of the 2 species. In the current study, we tested whether canine-derived gene-containing bacterial artificial chromosome (BAC) clones can be routinely used to build a gene-specific map of the red fox genome. Forty canine BAC clones were mapped to the red fox genome by fluorescence in situ hybridization (FISH). Each clone was uniquely assigned to a single fox chromosome, and the locations of 38 clones agreed with cytogenetic predictions. These results clearly demonstrate the utility of FISH mapping for construction of a whole-genome gene-specific map of the red fox. The further possibility of using canine BAC clones to map genes in the American mink (Mustela vison) genome was also explored. Much lower success was obtained for this more distantly related farm-bred species, although a few BAC clones were mapped to the predicted chromosomal locations. Article in Journal/Newspaper Canis lupus Université de Rennes 1: Publications scientifiques (HAL) Journal of Heredity 100 suppl_1 S42 S53 |
institution |
Open Polar |
collection |
Université de Rennes 1: Publications scientifiques (HAL) |
op_collection_id |
ftunivrennes1hal |
language |
English |
topic |
[SDV.GEN]Life Sciences [q-bio]/Genetics [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] |
spellingShingle |
[SDV.GEN]Life Sciences [q-bio]/Genetics [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Kukekova, Anna, Vorobieva, Nadegda, Beklemisheva, Violetta, Johnson, Jennifer, Temnykh, Svetlana, Yudkin, Dmitry, Trut, Lyudmila, André, Catherine Galibert, Francis Aguirre, Gustavo, Acland, Gregory, Graphodatsky, Alexander Chromosomal mapping of canine-derived BAC clones to the red fox and American mink genomes. |
topic_facet |
[SDV.GEN]Life Sciences [q-bio]/Genetics [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] |
description |
International audience High-quality sequencing of the dog (Canis lupus familiaris) genome has enabled enormous progress in genetic mapping of canine phenotypic variation. The red fox (Vulpes vulpes), another canid species, also exhibits a wide range of variation in coat color, morphology, and behavior. Although the fox genome has not yet been sequenced, canine genomic resources have been used to construct a meiotic linkage map of the red fox genome and begin genetic mapping in foxes. However, a more detailed gene-specific comparative map between the dog and fox genomes is required to establish gene order within homologous regions of dog and fox chromosomes and to refine breakpoints between homologous chromosomes of the 2 species. In the current study, we tested whether canine-derived gene-containing bacterial artificial chromosome (BAC) clones can be routinely used to build a gene-specific map of the red fox genome. Forty canine BAC clones were mapped to the red fox genome by fluorescence in situ hybridization (FISH). Each clone was uniquely assigned to a single fox chromosome, and the locations of 38 clones agreed with cytogenetic predictions. These results clearly demonstrate the utility of FISH mapping for construction of a whole-genome gene-specific map of the red fox. The further possibility of using canine BAC clones to map genes in the American mink (Mustela vison) genome was also explored. Much lower success was obtained for this more distantly related farm-bred species, although a few BAC clones were mapped to the predicted chromosomal locations. |
author2 |
J. A. Baker Institute Cornell University New York Institute of Cytology and Genetics Russian Academy of Sciences Moscow (RAS) Institut de Génétique et Développement de Rennes (IGDR) Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS) Department of Clinical Studies University of Pennsylvania |
format |
Article in Journal/Newspaper |
author |
Kukekova, Anna, Vorobieva, Nadegda, Beklemisheva, Violetta, Johnson, Jennifer, Temnykh, Svetlana, Yudkin, Dmitry, Trut, Lyudmila, André, Catherine Galibert, Francis Aguirre, Gustavo, Acland, Gregory, Graphodatsky, Alexander |
author_facet |
Kukekova, Anna, Vorobieva, Nadegda, Beklemisheva, Violetta, Johnson, Jennifer, Temnykh, Svetlana, Yudkin, Dmitry, Trut, Lyudmila, André, Catherine Galibert, Francis Aguirre, Gustavo, Acland, Gregory, Graphodatsky, Alexander |
author_sort |
Kukekova, Anna, |
title |
Chromosomal mapping of canine-derived BAC clones to the red fox and American mink genomes. |
title_short |
Chromosomal mapping of canine-derived BAC clones to the red fox and American mink genomes. |
title_full |
Chromosomal mapping of canine-derived BAC clones to the red fox and American mink genomes. |
title_fullStr |
Chromosomal mapping of canine-derived BAC clones to the red fox and American mink genomes. |
title_full_unstemmed |
Chromosomal mapping of canine-derived BAC clones to the red fox and American mink genomes. |
title_sort |
chromosomal mapping of canine-derived bac clones to the red fox and american mink genomes. |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://www.hal.inserm.fr/inserm-00405774 https://doi.org/10.1093/jhered/esp037 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
ISSN: 0022-1503 EISSN: 1471-8505 Journal of Heredity https://www.hal.inserm.fr/inserm-00405774 Journal of Heredity, 2009, 100 Suppl 1, pp.S42-53. ⟨10.1093/jhered/esp037⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1093/jhered/esp037 info:eu-repo/semantics/altIdentifier/pmid/19546120 inserm-00405774 https://www.hal.inserm.fr/inserm-00405774 doi:10.1093/jhered/esp037 PUBMED: 19546120 PUBMEDCENTRAL: PMC3139363 |
op_doi |
https://doi.org/10.1093/jhered/esp037 |
container_title |
Journal of Heredity |
container_volume |
100 |
container_issue |
suppl_1 |
container_start_page |
S42 |
op_container_end_page |
S53 |
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1774716563393347584 |