Cloning, characterization, and comparative activity of turbot IGF-I and IGF-II
International audience IGF peptides belong to a complex system that is known to play a major role in the control of growth and development in mammals. Even if studies performed in nonmammalian species tend to demonstrate an important function of these molecules, use of heterologous ligands, especial...
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Online Access: | https://hal.inrae.fr/hal-02677014 https://doi.org/10.1016/S0016-6480(02)00002-3 |
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ftunivrennes1hal:oai:HAL:hal-02677014v1 2023-12-24T10:25:27+01:00 Cloning, characterization, and comparative activity of turbot IGF-I and IGF-II Duval, Henri Rousseau, K. Eliès, G. Le Bail, Pierre-Yves Dufour, S. Boeuf, Gilles Boujard, Daniel Interactions cellulaires et moléculaires (ICM) Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Physiologie Générale et Comparée (LPGC) Centre National de la Recherche Scientifique (CNRS) Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) Institut National de la Recherche Agronomique (INRA) 2002 https://hal.inrae.fr/hal-02677014 https://doi.org/10.1016/S0016-6480(02)00002-3 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/S0016-6480(02)00002-3 info:eu-repo/semantics/altIdentifier/pmid/12093114 hal-02677014 https://hal.inrae.fr/hal-02677014 doi:10.1016/S0016-6480(02)00002-3 PRODINRA: 59768 PUBMED: 12093114 ISSN: 0016-6480 EISSN: 1095-6840 General and Comparative Endocrinology https://hal.inrae.fr/hal-02677014 General and Comparative Endocrinology, 2002, 126, pp.269-278. ⟨10.1016/S0016-6480(02)00002-3⟩ https://www.sciencedirect.com/science/journal/00166480 IGF-1 IGF-2 in vitro GH release expression in E.coli [SDV]Life Sciences [q-bio] [INFO]Computer Science [cs] info:eu-repo/semantics/article Journal articles 2002 ftunivrennes1hal https://doi.org/10.1016/S0016-6480(02)00002-3 2023-11-28T23:46:54Z International audience IGF peptides belong to a complex system that is known to play a major role in the control of growth and development in mammals. Even if studies performed in nonmammalian species tend to demonstrate an important function of these molecules, use of heterologous ligands, especially in fish, partly limit our knowledge of the physiological role(s) of IGFs. We report in this study the cloning, production, and characterization in an evolved fish, the turbot Psetta maxima, of mature IGF-I and IGF-II. The deduced 68-amino-acid IGF-I and 70-amino-acid IGF-II show 75% and 74% sequence identity with their mammalian counterparts, respectively, confirming the high sequence homology observed in other species. The development of a simple and efficient system for the production and purification of both IGF-I and IGF-II in Escherichia coli was used to investigate the in vitro regulation of GH release in the turbot. Our results demonstrated for the first time in a Euteleost species that both peptides specifically inhibited GH release. Both hormones were equally potent in inhibiting GH release from dispersed pituitary cells, with maximal inhibitory effects of 92% and 91% at 1 nM doses after 12 days of culture, respectively. The biologically active recombinant turbot IGFs that we obtained will allow us to further investigate potential and perhaps the specific role(s) of these hormones in turbot as, in contrast with mammals, growth in fish is potentially continued during “adult” life. Article in Journal/Newspaper Turbot Université de Rennes 1: Publications scientifiques (HAL) General and Comparative Endocrinology 126 3 269 278 |
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Open Polar |
collection |
Université de Rennes 1: Publications scientifiques (HAL) |
op_collection_id |
ftunivrennes1hal |
language |
English |
topic |
IGF-1 IGF-2 in vitro GH release expression in E.coli [SDV]Life Sciences [q-bio] [INFO]Computer Science [cs] |
spellingShingle |
IGF-1 IGF-2 in vitro GH release expression in E.coli [SDV]Life Sciences [q-bio] [INFO]Computer Science [cs] Duval, Henri Rousseau, K. Eliès, G. Le Bail, Pierre-Yves Dufour, S. Boeuf, Gilles Boujard, Daniel Cloning, characterization, and comparative activity of turbot IGF-I and IGF-II |
topic_facet |
IGF-1 IGF-2 in vitro GH release expression in E.coli [SDV]Life Sciences [q-bio] [INFO]Computer Science [cs] |
description |
International audience IGF peptides belong to a complex system that is known to play a major role in the control of growth and development in mammals. Even if studies performed in nonmammalian species tend to demonstrate an important function of these molecules, use of heterologous ligands, especially in fish, partly limit our knowledge of the physiological role(s) of IGFs. We report in this study the cloning, production, and characterization in an evolved fish, the turbot Psetta maxima, of mature IGF-I and IGF-II. The deduced 68-amino-acid IGF-I and 70-amino-acid IGF-II show 75% and 74% sequence identity with their mammalian counterparts, respectively, confirming the high sequence homology observed in other species. The development of a simple and efficient system for the production and purification of both IGF-I and IGF-II in Escherichia coli was used to investigate the in vitro regulation of GH release in the turbot. Our results demonstrated for the first time in a Euteleost species that both peptides specifically inhibited GH release. Both hormones were equally potent in inhibiting GH release from dispersed pituitary cells, with maximal inhibitory effects of 92% and 91% at 1 nM doses after 12 days of culture, respectively. The biologically active recombinant turbot IGFs that we obtained will allow us to further investigate potential and perhaps the specific role(s) of these hormones in turbot as, in contrast with mammals, growth in fish is potentially continued during “adult” life. |
author2 |
Interactions cellulaires et moléculaires (ICM) Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Physiologie Générale et Comparée (LPGC) Centre National de la Recherche Scientifique (CNRS) Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) Institut National de la Recherche Agronomique (INRA) |
format |
Article in Journal/Newspaper |
author |
Duval, Henri Rousseau, K. Eliès, G. Le Bail, Pierre-Yves Dufour, S. Boeuf, Gilles Boujard, Daniel |
author_facet |
Duval, Henri Rousseau, K. Eliès, G. Le Bail, Pierre-Yves Dufour, S. Boeuf, Gilles Boujard, Daniel |
author_sort |
Duval, Henri |
title |
Cloning, characterization, and comparative activity of turbot IGF-I and IGF-II |
title_short |
Cloning, characterization, and comparative activity of turbot IGF-I and IGF-II |
title_full |
Cloning, characterization, and comparative activity of turbot IGF-I and IGF-II |
title_fullStr |
Cloning, characterization, and comparative activity of turbot IGF-I and IGF-II |
title_full_unstemmed |
Cloning, characterization, and comparative activity of turbot IGF-I and IGF-II |
title_sort |
cloning, characterization, and comparative activity of turbot igf-i and igf-ii |
publisher |
HAL CCSD |
publishDate |
2002 |
url |
https://hal.inrae.fr/hal-02677014 https://doi.org/10.1016/S0016-6480(02)00002-3 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
ISSN: 0016-6480 EISSN: 1095-6840 General and Comparative Endocrinology https://hal.inrae.fr/hal-02677014 General and Comparative Endocrinology, 2002, 126, pp.269-278. ⟨10.1016/S0016-6480(02)00002-3⟩ https://www.sciencedirect.com/science/journal/00166480 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0016-6480(02)00002-3 info:eu-repo/semantics/altIdentifier/pmid/12093114 hal-02677014 https://hal.inrae.fr/hal-02677014 doi:10.1016/S0016-6480(02)00002-3 PRODINRA: 59768 PUBMED: 12093114 |
op_doi |
https://doi.org/10.1016/S0016-6480(02)00002-3 |
container_title |
General and Comparative Endocrinology |
container_volume |
126 |
container_issue |
3 |
container_start_page |
269 |
op_container_end_page |
278 |
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1786201240259002368 |