Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides
International audience Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 mu g/L), isoproturon (0.1 and 1 mu g/L), or both in a mixture (0.2 and 0...
Published in: | Chemical Research in Toxicology |
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Online Access: | https://hal-univ-rennes1.archives-ouvertes.fr/hal-02527813 https://doi.org/10.1021/acs.chemrestox.5b00269 |
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ftunivrennes1hal:oai:HAL:hal-02527813v1 2023-05-15T15:58:08+02:00 Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides Epelboin, Yanouk Quéré, Claudie Pernet, Fabrice Pichereau, Vianney Corporeau, Charlotte Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2015-09-02 https://hal-univ-rennes1.archives-ouvertes.fr/hal-02527813 https://doi.org/10.1021/acs.chemrestox.5b00269 en eng HAL CCSD American Chemical Society info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.chemrestox.5b00269 hal-02527813 https://hal-univ-rennes1.archives-ouvertes.fr/hal-02527813 doi:10.1021/acs.chemrestox.5b00269 ISSN: 0893-228X EISSN: 1520-5010 Chemical Research in Toxicology https://hal-univ-rennes1.archives-ouvertes.fr/hal-02527813 Chemical Research in Toxicology, 2015, 28 (9), pp.1831-1841. ⟨10.1021/acs.chemrestox.5b00269⟩ ACTIVATED PROTEIN-KINASE CRASSOSTREA-GIGAS MOLECULAR-IDENTIFICATION PROTEOMIC RESPONSE OXIDATIVE STRESS CELLULAR-ENERGY PECTEN-MAXIMUS FATTY-ACIDS DIURON PARAMETERS ACL [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2015 ftunivrennes1hal https://doi.org/10.1021/acs.chemrestox.5b00269 2023-03-22T00:04:15Z International audience Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 mu g/L), isoproturon (0.1 and 1 mu g/L), or both in a mixture (0.2 and 0.1 mu g/L, respectively). Exposure to trace levels of pesticides had no effect on the filtration rate, growth, and energy reserves of oysters. However, oysters exposed to metconazole and isoproturon showed an overactivation of the sensing-kinase AMP-activated protein kinase alpha (AMPK alpha), a key enzyme involved in energy metabolism and more particularly glycolysis. In the meantime, these exposed oysters showed a decrease in hexokinase and pyruvate kinase activities, whereas 2-DE proteomic revealed that fructose-1,6-bisphosphatase (F-1,6-BP), a key enzyme of gluconeogenesis, was upregulated. Activities of antioxidant enzymes were higher in oysters exposed to the highest pesticide concentrations. Both pesticides enhanced the superoxide dismutase activity of oysters. Isoproturon enhanced catalase activity, and metconazole enhanced peroxiredoxin activity. Overall, our results show that environmental concentrations of metconazole or isoproturon induced subtle changes in the energy and antioxidant metabolisms of oysters. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Rennes 1: Publications scientifiques (HAL) Pacific Chemical Research in Toxicology 28 9 1831 1841 |
institution |
Open Polar |
collection |
Université de Rennes 1: Publications scientifiques (HAL) |
op_collection_id |
ftunivrennes1hal |
language |
English |
topic |
ACTIVATED PROTEIN-KINASE CRASSOSTREA-GIGAS MOLECULAR-IDENTIFICATION PROTEOMIC RESPONSE OXIDATIVE STRESS CELLULAR-ENERGY PECTEN-MAXIMUS FATTY-ACIDS DIURON PARAMETERS ACL [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACTIVATED PROTEIN-KINASE CRASSOSTREA-GIGAS MOLECULAR-IDENTIFICATION PROTEOMIC RESPONSE OXIDATIVE STRESS CELLULAR-ENERGY PECTEN-MAXIMUS FATTY-ACIDS DIURON PARAMETERS ACL [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology Epelboin, Yanouk Quéré, Claudie Pernet, Fabrice Pichereau, Vianney Corporeau, Charlotte Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
topic_facet |
ACTIVATED PROTEIN-KINASE CRASSOSTREA-GIGAS MOLECULAR-IDENTIFICATION PROTEOMIC RESPONSE OXIDATIVE STRESS CELLULAR-ENERGY PECTEN-MAXIMUS FATTY-ACIDS DIURON PARAMETERS ACL [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 mu g/L), isoproturon (0.1 and 1 mu g/L), or both in a mixture (0.2 and 0.1 mu g/L, respectively). Exposure to trace levels of pesticides had no effect on the filtration rate, growth, and energy reserves of oysters. However, oysters exposed to metconazole and isoproturon showed an overactivation of the sensing-kinase AMP-activated protein kinase alpha (AMPK alpha), a key enzyme involved in energy metabolism and more particularly glycolysis. In the meantime, these exposed oysters showed a decrease in hexokinase and pyruvate kinase activities, whereas 2-DE proteomic revealed that fructose-1,6-bisphosphatase (F-1,6-BP), a key enzyme of gluconeogenesis, was upregulated. Activities of antioxidant enzymes were higher in oysters exposed to the highest pesticide concentrations. Both pesticides enhanced the superoxide dismutase activity of oysters. Isoproturon enhanced catalase activity, and metconazole enhanced peroxiredoxin activity. Overall, our results show that environmental concentrations of metconazole or isoproturon induced subtle changes in the energy and antioxidant metabolisms of oysters. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Epelboin, Yanouk Quéré, Claudie Pernet, Fabrice Pichereau, Vianney Corporeau, Charlotte |
author_facet |
Epelboin, Yanouk Quéré, Claudie Pernet, Fabrice Pichereau, Vianney Corporeau, Charlotte |
author_sort |
Epelboin, Yanouk |
title |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_short |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_full |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_fullStr |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_full_unstemmed |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_sort |
energy and antioxidant responses of pacific oyster exposed to trace levels of pesticides |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal-univ-rennes1.archives-ouvertes.fr/hal-02527813 https://doi.org/10.1021/acs.chemrestox.5b00269 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0893-228X EISSN: 1520-5010 Chemical Research in Toxicology https://hal-univ-rennes1.archives-ouvertes.fr/hal-02527813 Chemical Research in Toxicology, 2015, 28 (9), pp.1831-1841. ⟨10.1021/acs.chemrestox.5b00269⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.chemrestox.5b00269 hal-02527813 https://hal-univ-rennes1.archives-ouvertes.fr/hal-02527813 doi:10.1021/acs.chemrestox.5b00269 |
op_doi |
https://doi.org/10.1021/acs.chemrestox.5b00269 |
container_title |
Chemical Research in Toxicology |
container_volume |
28 |
container_issue |
9 |
container_start_page |
1831 |
op_container_end_page |
1841 |
_version_ |
1766393866602151936 |