Overcoming deterrent metabolites by gaining essential nutrients A lichen/snail case study
International audience Specialised metabolites in lichens are generally considered repellent compounds by consumers. Nevertheless, if the only food available is lichens rich in specialised metabolites, lichenophages must implement strategies to overcome the toxicity of these metabolites. Thus, the b...
Published in: | Phytochemistry |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2019
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Online Access: | https://univ-rennes.hal.science/hal-02150227 https://univ-rennes.hal.science/hal-02150227/document https://univ-rennes.hal.science/hal-02150227/file/Gadea%20et%20al-2019-Overcoming%20deterrent%20metabolites%20by%20gaining%20essential%20nutrients-draft_revised_without%20TC.pdf https://doi.org/10.1016/j.phytochem.2019.04.019 |
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ftunivrennes1hal:oai:HAL:hal-02150227v1 |
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openpolar |
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Open Polar |
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Université de Rennes 1: Publications scientifiques (HAL) |
op_collection_id |
ftunivrennes1hal |
language |
English |
topic |
Mass spectrometry imaging Lichen Feeding choice D-arabitol Notodiscus hookeri Parmeliaceae Snail Usnic acid Usnea taylorii [SDV.IB]Life Sciences [q-bio]/Bioengineering |
spellingShingle |
Mass spectrometry imaging Lichen Feeding choice D-arabitol Notodiscus hookeri Parmeliaceae Snail Usnic acid Usnea taylorii [SDV.IB]Life Sciences [q-bio]/Bioengineering Gadea, Alice Charrier, Maryvonne Fanuel, Mathieu Clerc, Philippe Daugan, Corentin Sauvager, Aurélie Rogniaux, Hélène Boustie, Joël Le Lamer, Anne-Cécile Lohezic-Le Devehat, Françoise Overcoming deterrent metabolites by gaining essential nutrients A lichen/snail case study |
topic_facet |
Mass spectrometry imaging Lichen Feeding choice D-arabitol Notodiscus hookeri Parmeliaceae Snail Usnic acid Usnea taylorii [SDV.IB]Life Sciences [q-bio]/Bioengineering |
description |
International audience Specialised metabolites in lichens are generally considered repellent compounds by consumers. Nevertheless, if the only food available is lichens rich in specialised metabolites, lichenophages must implement strategies to overcome the toxicity of these metabolites. Thus, the balance between phagostimulant nutrients and deterrent metabolites could play a key role in feeding preferences. To further understand lichen-gastropod interactions, we studied the feeding behaviour and consumption in Notodiscus hookeri, the land snail native to sub-Antarctic islands. The lichen Usnea taylorii was used because of its simple chemistry, its richness in usnic acid (specialised metabolite) and arabitol (primary metabolite) and its presence in snail habitats. Choice tests in arenas with intact lichens versus acetone-rinsed lichens were carried out to study the influence of specialised metabolites on snail behaviour and feeding preference. Simultaneously, usnic acid and arabitol were quantified and located within the lichen thallus using HPLC-DAD-MS and in situ imaging by mass spectrometry to assess whether their spatial distribution explained preferential snail grazing. No-choice feeding experiments, with the pure metabolites embedded in an artificial diet, defined a gradual gustatory response, from strong repellence (usnic acid) to high appetence (D-arabitol). This case study demonstrates that the nutritional activity of N. hookeri is governed by the chemical quality of the food and primarily by nutrient availability (arabitol), despite the presence of deterrent metabolite (usnic acid). |
author2 |
Ecosystèmes, biodiversité, évolution Rennes (ECOBIO) Université de Rennes (UR)-Institut Ecologie et Environnement - CNRS Ecologie et Environnement (INEE-CNRS) Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR) Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS) Institut des Sciences Chimiques de Rennes (ISCR) Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes) Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA) Institut National de la Recherche Agronomique (INRA) Conservatoire et Jardin Botaniques Pharmacochimie et Biologie pour le Développement (PHARMA-DEV) Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT) Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT) Subantarctic field trip was funded by l'Institut Polaire Paul-Émile Victor, Plouzané, France (IPEV, programme 136). |
format |
Article in Journal/Newspaper |
author |
Gadea, Alice Charrier, Maryvonne Fanuel, Mathieu Clerc, Philippe Daugan, Corentin Sauvager, Aurélie Rogniaux, Hélène Boustie, Joël Le Lamer, Anne-Cécile Lohezic-Le Devehat, Françoise |
author_facet |
Gadea, Alice Charrier, Maryvonne Fanuel, Mathieu Clerc, Philippe Daugan, Corentin Sauvager, Aurélie Rogniaux, Hélène Boustie, Joël Le Lamer, Anne-Cécile Lohezic-Le Devehat, Françoise |
author_sort |
Gadea, Alice |
title |
Overcoming deterrent metabolites by gaining essential nutrients A lichen/snail case study |
title_short |
Overcoming deterrent metabolites by gaining essential nutrients A lichen/snail case study |
title_full |
Overcoming deterrent metabolites by gaining essential nutrients A lichen/snail case study |
title_fullStr |
Overcoming deterrent metabolites by gaining essential nutrients A lichen/snail case study |
title_full_unstemmed |
Overcoming deterrent metabolites by gaining essential nutrients A lichen/snail case study |
title_sort |
overcoming deterrent metabolites by gaining essential nutrients a lichen/snail case study |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://univ-rennes.hal.science/hal-02150227 https://univ-rennes.hal.science/hal-02150227/document https://univ-rennes.hal.science/hal-02150227/file/Gadea%20et%20al-2019-Overcoming%20deterrent%20metabolites%20by%20gaining%20essential%20nutrients-draft_revised_without%20TC.pdf https://doi.org/10.1016/j.phytochem.2019.04.019 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
ISSN: 0031-9422 Phytochemistry https://univ-rennes.hal.science/hal-02150227 Phytochemistry, 2019, 164, pp.86-93. ⟨10.1016/j.phytochem.2019.04.019⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phytochem.2019.04.019 info:eu-repo/semantics/altIdentifier/pmid/31102999 hal-02150227 https://univ-rennes.hal.science/hal-02150227 https://univ-rennes.hal.science/hal-02150227/document https://univ-rennes.hal.science/hal-02150227/file/Gadea%20et%20al-2019-Overcoming%20deterrent%20metabolites%20by%20gaining%20essential%20nutrients-draft_revised_without%20TC.pdf doi:10.1016/j.phytochem.2019.04.019 PUBMED: 31102999 WOS: 000475996800010 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.phytochem.2019.04.019 |
container_title |
Phytochemistry |
container_volume |
164 |
container_start_page |
86 |
op_container_end_page |
93 |
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1798850152745664512 |
spelling |
ftunivrennes1hal:oai:HAL:hal-02150227v1 2024-05-12T07:54:33+00:00 Overcoming deterrent metabolites by gaining essential nutrients A lichen/snail case study Gadea, Alice Charrier, Maryvonne Fanuel, Mathieu Clerc, Philippe Daugan, Corentin Sauvager, Aurélie Rogniaux, Hélène Boustie, Joël Le Lamer, Anne-Cécile Lohezic-Le Devehat, Françoise Ecosystèmes, biodiversité, évolution Rennes (ECOBIO) Université de Rennes (UR)-Institut Ecologie et Environnement - CNRS Ecologie et Environnement (INEE-CNRS) Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR) Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS) Institut des Sciences Chimiques de Rennes (ISCR) Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes) Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA) Institut National de la Recherche Agronomique (INRA) Conservatoire et Jardin Botaniques Pharmacochimie et Biologie pour le Développement (PHARMA-DEV) Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT) Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT) Subantarctic field trip was funded by l'Institut Polaire Paul-Émile Victor, Plouzané, France (IPEV, programme 136). 2019-08 https://univ-rennes.hal.science/hal-02150227 https://univ-rennes.hal.science/hal-02150227/document https://univ-rennes.hal.science/hal-02150227/file/Gadea%20et%20al-2019-Overcoming%20deterrent%20metabolites%20by%20gaining%20essential%20nutrients-draft_revised_without%20TC.pdf https://doi.org/10.1016/j.phytochem.2019.04.019 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phytochem.2019.04.019 info:eu-repo/semantics/altIdentifier/pmid/31102999 hal-02150227 https://univ-rennes.hal.science/hal-02150227 https://univ-rennes.hal.science/hal-02150227/document https://univ-rennes.hal.science/hal-02150227/file/Gadea%20et%20al-2019-Overcoming%20deterrent%20metabolites%20by%20gaining%20essential%20nutrients-draft_revised_without%20TC.pdf doi:10.1016/j.phytochem.2019.04.019 PUBMED: 31102999 WOS: 000475996800010 info:eu-repo/semantics/OpenAccess ISSN: 0031-9422 Phytochemistry https://univ-rennes.hal.science/hal-02150227 Phytochemistry, 2019, 164, pp.86-93. ⟨10.1016/j.phytochem.2019.04.019⟩ Mass spectrometry imaging Lichen Feeding choice D-arabitol Notodiscus hookeri Parmeliaceae Snail Usnic acid Usnea taylorii [SDV.IB]Life Sciences [q-bio]/Bioengineering info:eu-repo/semantics/article Journal articles 2019 ftunivrennes1hal https://doi.org/10.1016/j.phytochem.2019.04.019 2024-04-18T00:18:46Z International audience Specialised metabolites in lichens are generally considered repellent compounds by consumers. Nevertheless, if the only food available is lichens rich in specialised metabolites, lichenophages must implement strategies to overcome the toxicity of these metabolites. Thus, the balance between phagostimulant nutrients and deterrent metabolites could play a key role in feeding preferences. To further understand lichen-gastropod interactions, we studied the feeding behaviour and consumption in Notodiscus hookeri, the land snail native to sub-Antarctic islands. The lichen Usnea taylorii was used because of its simple chemistry, its richness in usnic acid (specialised metabolite) and arabitol (primary metabolite) and its presence in snail habitats. Choice tests in arenas with intact lichens versus acetone-rinsed lichens were carried out to study the influence of specialised metabolites on snail behaviour and feeding preference. Simultaneously, usnic acid and arabitol were quantified and located within the lichen thallus using HPLC-DAD-MS and in situ imaging by mass spectrometry to assess whether their spatial distribution explained preferential snail grazing. No-choice feeding experiments, with the pure metabolites embedded in an artificial diet, defined a gradual gustatory response, from strong repellence (usnic acid) to high appetence (D-arabitol). This case study demonstrates that the nutritional activity of N. hookeri is governed by the chemical quality of the food and primarily by nutrient availability (arabitol), despite the presence of deterrent metabolite (usnic acid). Article in Journal/Newspaper Antarc* Antarctic Université de Rennes 1: Publications scientifiques (HAL) Antarctic Phytochemistry 164 86 93 |