Comparative analysis of hemocyte properties from Mytilus edulis desolationis and Aulacomya ater in the Kerguelen Islands.

• Published in: Marine Environmental Research
• Main Authors: Caza, France, Betoulle, Stéphane, Auffret, Michel, Brousseau, Pauline, Fournier, Michel, St-Pierre, Yves
• Other Authors: Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique Québec (INRS)-Réseau International des Instituts Pasteur (RIIP), Université de Reims Champagne-Ardenne (URCA), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), IMMUNOTOXKER IPEV Project 409, Supported by the National Science and Engineering Council of Canada (NSERC) STPGP 430539, the Agence Nationale de laRecherche (ANR), the French Polar Institute Paul Emile Victor (IPEV Project N°409 IMMUNOTOXKER), and the Terres Australes et Antarctiques Françaises (TAAF)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2015
• Subjects: Cadmium; Apoptosis; Hemocytes; Flow cytometry; Kerguelen; Phagocytosis; ACL; [SDV]Life Sciences [q-bio]; [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology; [SDE.MCG]Environmental Sciences/Global Changes; [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; Kerguelen Islands
• Online Access: https://riip.hal.science/pasteur-01351903; https://doi.org/10.1016/j.marenvres.2015.09.003

International audience The capability of bivalve molluscs to respond to environmental stresses largely depends upon their cellular immunity. Accordingly, shift in habitat conditions following thermal stress or exposure to pollutants may harm sensitive species differently, thereby modulating the biodiversity of a given ecosystem by favoring stress-tolerant species. Here, we have compared the sensitivity of hemocytes from Mytilus edulis desolationis (M. edulis desolationis) and Aulacomya ater (A. ater) to acute thermal stress and exposure to cadmium. The two subantarctic species are commonly found in the same habitat in the isolated Kerguelen archipelago. Our results showed that the phagocytic activity and viability of hemocytes from both species were equally sensitive to increasing concentrations of cadmium. However, although in vitro exposure to cadmium induced apoptosis in hemocytes of M. edulis desolationis and A. ater, flow cytometric analyses showed that the apoptotic profile of both species differed greatly when using Annexin V and YO-PRO-1 as apoptotic markers. We also found that the total hemocyte counts decreased strongly in A. ater but not in M. edulis desolationis following an acute thermal stress. Taken together, these results showed that stress responses differed significantly in hemocytes from both species. This suggests that the co-existence of both species may be at risk following exposure to pollutants and/or changes in temperature.