Catalasic activity in fish liver: improvement of the UV to visible analytic method.
International audience Antioxidative defenses and more especially catalasic activity (CAT) are studied in a large range of scientific research thematics. In environmental sciences, the problematic of oxidative stress is of great interest as pollutants can induce perturbations of redox homeostasis. C...
Published in: | Fish Physiology and Biochemistry |
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ftunivreimsca:oai:HAL:hal-01576096v1 2023-12-31T09:59:02+01:00 Catalasic activity in fish liver: improvement of the UV to visible analytic method. Paris-Palacios, Séverine Delahaut, Laurence Carreras, Alexis Thomas, Marielle Biagianti-Risbourg, Sylvie Unité de Recherche Interactions Animal-Environnement - EA 4689 (IAE) Université de Reims Champagne-Ardenne (URCA) Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA) Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) 2013 https://hal.univ-lorraine.fr/hal-01576096 https://doi.org/10.1007/s10695-012-9754-6 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10695-012-9754-6 info:eu-repo/semantics/altIdentifier/pmid/23224832 hal-01576096 https://hal.univ-lorraine.fr/hal-01576096 doi:10.1007/s10695-012-9754-6 PUBMED: 23224832 ISSN: 0920-1742 EISSN: 1573-5168 Fish Physiology and Biochemistry https://hal.univ-lorraine.fr/hal-01576096 Fish Physiology and Biochemistry, 2013, 39 (4), pp.957-966. ⟨10.1007/s10695-012-9754-6⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2013 ftunivreimsca https://doi.org/10.1007/s10695-012-9754-6 2023-12-05T23:52:03Z International audience Antioxidative defenses and more especially catalasic activity (CAT) are studied in a large range of scientific research thematics. In environmental sciences, the problematic of oxidative stress is of great interest as pollutants can induce perturbations of redox homeostasis. Consequently, changes in antioxidative defenses levels in fish tissues and particularly in liver are used as potential biomarkers of pollution. In most studies, the CAT was assayed by following during 5 min the consumption of H2O2 in cytosolic buffered extracts at 240 nm (UV-method). This study proposed a development of this method in the visible, using permanganate and a 525-nm detection, which was more accurate, sensitive, and rapid. Moreover, the hepatic CAT of six different fish species [a cyclidae (Nimbochromis linni), 3 cyprinidae (Brachydanio rerio, Rutilus rutilus, Cyprinus carpio), an anguillidae (Anguilla anguilla), and a percidae (Perca fluviatilus)] was evaluated with the two protocols (UV- and KMnO4-method). The results but also the thermal optimum of the reaction and the interest of CAT as biomarker in ecotoxicology were discussed. Article in Journal/Newspaper Anguilla anguilla Université de Reims Champagne-Ardenne: Archives Ouvertes (HAL) Fish Physiology and Biochemistry 39 4 957 966 |
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Université de Reims Champagne-Ardenne: Archives Ouvertes (HAL) |
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[SDV]Life Sciences [q-bio] |
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[SDV]Life Sciences [q-bio] Paris-Palacios, Séverine Delahaut, Laurence Carreras, Alexis Thomas, Marielle Biagianti-Risbourg, Sylvie Catalasic activity in fish liver: improvement of the UV to visible analytic method. |
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[SDV]Life Sciences [q-bio] |
description |
International audience Antioxidative defenses and more especially catalasic activity (CAT) are studied in a large range of scientific research thematics. In environmental sciences, the problematic of oxidative stress is of great interest as pollutants can induce perturbations of redox homeostasis. Consequently, changes in antioxidative defenses levels in fish tissues and particularly in liver are used as potential biomarkers of pollution. In most studies, the CAT was assayed by following during 5 min the consumption of H2O2 in cytosolic buffered extracts at 240 nm (UV-method). This study proposed a development of this method in the visible, using permanganate and a 525-nm detection, which was more accurate, sensitive, and rapid. Moreover, the hepatic CAT of six different fish species [a cyclidae (Nimbochromis linni), 3 cyprinidae (Brachydanio rerio, Rutilus rutilus, Cyprinus carpio), an anguillidae (Anguilla anguilla), and a percidae (Perca fluviatilus)] was evaluated with the two protocols (UV- and KMnO4-method). The results but also the thermal optimum of the reaction and the interest of CAT as biomarker in ecotoxicology were discussed. |
author2 |
Unité de Recherche Interactions Animal-Environnement - EA 4689 (IAE) Université de Reims Champagne-Ardenne (URCA) Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA) Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) |
format |
Article in Journal/Newspaper |
author |
Paris-Palacios, Séverine Delahaut, Laurence Carreras, Alexis Thomas, Marielle Biagianti-Risbourg, Sylvie |
author_facet |
Paris-Palacios, Séverine Delahaut, Laurence Carreras, Alexis Thomas, Marielle Biagianti-Risbourg, Sylvie |
author_sort |
Paris-Palacios, Séverine |
title |
Catalasic activity in fish liver: improvement of the UV to visible analytic method. |
title_short |
Catalasic activity in fish liver: improvement of the UV to visible analytic method. |
title_full |
Catalasic activity in fish liver: improvement of the UV to visible analytic method. |
title_fullStr |
Catalasic activity in fish liver: improvement of the UV to visible analytic method. |
title_full_unstemmed |
Catalasic activity in fish liver: improvement of the UV to visible analytic method. |
title_sort |
catalasic activity in fish liver: improvement of the uv to visible analytic method. |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.univ-lorraine.fr/hal-01576096 https://doi.org/10.1007/s10695-012-9754-6 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
ISSN: 0920-1742 EISSN: 1573-5168 Fish Physiology and Biochemistry https://hal.univ-lorraine.fr/hal-01576096 Fish Physiology and Biochemistry, 2013, 39 (4), pp.957-966. ⟨10.1007/s10695-012-9754-6⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10695-012-9754-6 info:eu-repo/semantics/altIdentifier/pmid/23224832 hal-01576096 https://hal.univ-lorraine.fr/hal-01576096 doi:10.1007/s10695-012-9754-6 PUBMED: 23224832 |
op_doi |
https://doi.org/10.1007/s10695-012-9754-6 |
container_title |
Fish Physiology and Biochemistry |
container_volume |
39 |
container_issue |
4 |
container_start_page |
957 |
op_container_end_page |
966 |
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1786822914192965632 |