Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions
International audience Pesticide run-off into the ocean represents a potential threat to marine organisms, especially bivalves living in coastal environments. However, little is known about the effects of environmentally relevant concentrations of pesticides at the individual level. In this study, t...
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Online Access: | https://hal.univ-brest.fr/hal-00669935 https://hal.univ-brest.fr/hal-00669935/document https://hal.univ-brest.fr/hal-00669935/file/4010.full.pdf https://doi.org/10.1242/jeb.048033 |
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ftunivreimsca:oai:HAL:hal-00669935v1 2024-02-11T10:03:07+01:00 Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions Collin, Hélène Meistertzheim, Anne-Leila David, Elise Moraga, Dario Boutet, Isabelle Laboratoire Biologie de la Conservation Université de Lausanne = University of Lausanne (UNIL) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'Eco-Toxicologie Université de Reims Champagne-Ardenne (URCA) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2010 https://hal.univ-brest.fr/hal-00669935 https://hal.univ-brest.fr/hal-00669935/document https://hal.univ-brest.fr/hal-00669935/file/4010.full.pdf https://doi.org/10.1242/jeb.048033 en eng HAL CCSD The Company of Biologists info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.048033 hal-00669935 https://hal.univ-brest.fr/hal-00669935 https://hal.univ-brest.fr/hal-00669935/document https://hal.univ-brest.fr/hal-00669935/file/4010.full.pdf doi:10.1242/jeb.048033 info:eu-repo/semantics/OpenAccess ISSN: 0022-0949 EISSN: 1477-9145 Journal of Experimental Biology https://hal.univ-brest.fr/hal-00669935 Journal of Experimental Biology, 2010, 213 (23), pp.4010-4017. ⟨10.1242/jeb.048033⟩ SSH library pesticides C. gigas gene expression oxidative stress iron metabolism [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2010 ftunivreimsca https://doi.org/10.1242/jeb.048033 2024-01-23T23:44:03Z International audience Pesticide run-off into the ocean represents a potential threat to marine organisms, especially bivalves living in coastal environments. However, little is known about the effects of environmentally relevant concentrations of pesticides at the individual level. In this study, the suppression subtractive hybridisation technique was used to discover the main physiological function affected by a cocktail of three pesticides (lindane, metolachlor and carbofuran) in the Pacific oyster Crassostrea gigas. Two oyster populations exposed to different pollution levels in the wild were investigated. The pesticide concentrations used to induce stress were close to those found in the wild. In a time course experiment, the expression of three genes implicated in iron metabolism and oxidative stress as well as that of two ubiquitous stress proteins was examined. No clear regulation of gene or protein expression was found, potentially due to a low-dose effect. However, we detected a strong site-and organ-specific response to the pesticides. This study thus (1) provides insight into bivalve responses to pesticide pollution at the level of the transcriptome, which is the first level of response for organisms facing pollution, and (2) raises interesting questions concerning the importance of the sites and organs studied in the toxicogenomic field. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Reims Champagne-Ardenne: Archives Ouvertes (HAL) Pacific Journal of Experimental Biology 213 23 4010 4017 |
institution |
Open Polar |
collection |
Université de Reims Champagne-Ardenne: Archives Ouvertes (HAL) |
op_collection_id |
ftunivreimsca |
language |
English |
topic |
SSH library pesticides C. gigas gene expression oxidative stress iron metabolism [SDE]Environmental Sciences |
spellingShingle |
SSH library pesticides C. gigas gene expression oxidative stress iron metabolism [SDE]Environmental Sciences Collin, Hélène Meistertzheim, Anne-Leila David, Elise Moraga, Dario Boutet, Isabelle Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions |
topic_facet |
SSH library pesticides C. gigas gene expression oxidative stress iron metabolism [SDE]Environmental Sciences |
description |
International audience Pesticide run-off into the ocean represents a potential threat to marine organisms, especially bivalves living in coastal environments. However, little is known about the effects of environmentally relevant concentrations of pesticides at the individual level. In this study, the suppression subtractive hybridisation technique was used to discover the main physiological function affected by a cocktail of three pesticides (lindane, metolachlor and carbofuran) in the Pacific oyster Crassostrea gigas. Two oyster populations exposed to different pollution levels in the wild were investigated. The pesticide concentrations used to induce stress were close to those found in the wild. In a time course experiment, the expression of three genes implicated in iron metabolism and oxidative stress as well as that of two ubiquitous stress proteins was examined. No clear regulation of gene or protein expression was found, potentially due to a low-dose effect. However, we detected a strong site-and organ-specific response to the pesticides. This study thus (1) provides insight into bivalve responses to pesticide pollution at the level of the transcriptome, which is the first level of response for organisms facing pollution, and (2) raises interesting questions concerning the importance of the sites and organs studied in the toxicogenomic field. |
author2 |
Laboratoire Biologie de la Conservation Université de Lausanne = University of Lausanne (UNIL) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'Eco-Toxicologie Université de Reims Champagne-Ardenne (URCA) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Collin, Hélène Meistertzheim, Anne-Leila David, Elise Moraga, Dario Boutet, Isabelle |
author_facet |
Collin, Hélène Meistertzheim, Anne-Leila David, Elise Moraga, Dario Boutet, Isabelle |
author_sort |
Collin, Hélène |
title |
Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions |
title_short |
Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions |
title_full |
Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions |
title_fullStr |
Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions |
title_full_unstemmed |
Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions |
title_sort |
response of the pacific oyster crassostrea gigas, thunberg 1793, to pesticide exposure under experimental conditions |
publisher |
HAL CCSD |
publishDate |
2010 |
url |
https://hal.univ-brest.fr/hal-00669935 https://hal.univ-brest.fr/hal-00669935/document https://hal.univ-brest.fr/hal-00669935/file/4010.full.pdf https://doi.org/10.1242/jeb.048033 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0022-0949 EISSN: 1477-9145 Journal of Experimental Biology https://hal.univ-brest.fr/hal-00669935 Journal of Experimental Biology, 2010, 213 (23), pp.4010-4017. ⟨10.1242/jeb.048033⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.048033 hal-00669935 https://hal.univ-brest.fr/hal-00669935 https://hal.univ-brest.fr/hal-00669935/document https://hal.univ-brest.fr/hal-00669935/file/4010.full.pdf doi:10.1242/jeb.048033 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1242/jeb.048033 |
container_title |
Journal of Experimental Biology |
container_volume |
213 |
container_issue |
23 |
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4010 |
op_container_end_page |
4017 |
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