Shifts in rhizosphere microbial communities and enzyme activity of Poa alpina across an alpine chronosequence
This study quantifies the influence of Poa alpina on the soil microbial community in primary succession of alpine ecosystems, and whether these effects are controlled by the successional stage. Four successional sites representative of four stages of grassland development (initial, 4 years (non-vege...
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ftunivreading:oai:centaur.reading.ac.uk:4160 2023-09-05T13:22:42+02:00 Shifts in rhizosphere microbial communities and enzyme activity of Poa alpina across an alpine chronosequence Tscherko, D. Hammesfahr, U. Marx, M. C. Kandeler, E. 2003 https://centaur.reading.ac.uk/4160/ unknown Pergamon-Elsevier Science Ltd Tscherko, D., Hammesfahr, U., Marx, M. C. and Kandeler, E. (2003) Shifts in rhizosphere microbial communities and enzyme activity of Poa alpina across an alpine chronosequence. In: 2nd Enzymes in the Environment Conference, Prague, CZECH REPUBLIC, pp. 1685-1698. doi: https://doi.org/10.1016/j.soilbio.2004.07.004 <https://doi.org/10.1016/j.soilbio.2004.07.004> Conference or Workshop Item NonPeerReviewed 2003 ftunivreading https://doi.org/10.1016/j.soilbio.2004.07.004 2023-08-14T17:28:21Z This study quantifies the influence of Poa alpina on the soil microbial community in primary succession of alpine ecosystems, and whether these effects are controlled by the successional stage. Four successional sites representative of four stages of grassland development (initial, 4 years (non-vegetated); pioneer, 20 years; transition, 75 years; mature, 9500 years old) on the Rotmoos glacier foreland, Austria, were sampled. The size, composition and activity of the microbial community in the rhizosphere and bulk soil were characterized using the chloroform-fumigation extraction procedure, phospholipid fatty acid (PLFA) analysis and measurements of the enzymes beta-glucosidase, beta-xylosidase, N-acetyl-beta-glucosaminidase, leucine aminopeptidase, acid phosphatase and sulfatase. The interplay between the host plant and the successional stage was quantified using principal component (PCA) and multidimensional scaling analyses. Correlation analyses were applied to evaluate the relationship between soil factors (C-org, N-t, C/N ratio, pH, ammonium, phosphorus, potassium) and microbial properties in the bulk soil. In the pioneer stage microbial colonization of the rhizosphere of P. alpina was dependent on the reservoir of microbial species in the bulk soil. As a consequence, the rhizosphere and bulk soil were similar in microbial biomass (ninhydrin-reactive nitrogen (NHR-N)), community composition (PLFA), and enzyme activity. In the transition and mature grassland stage, more benign soil conditions stimulated microbial growth (NHR-N, total amount of PLFA, bacterial PLFA, Gram-positive bacteria, Gram-negative bacteria), and microbial diversity (Shannon index H) in the rhizosphere either directly or indirectly through enhanced carbon allocation. In the same period, the rhizosphere microflora shifted from a G(-) to a more G(+), and from a fungal to a more bacteria-dominated community. Rhizosphere beta-xylosidase, N-acetyl-beta-glucosaminidase, and sulfatase activity peaked in the mature grassland soil, whereas ... Conference Object Poa alpina CentAUR: Central Archive at the University of Reading Soil Biology and Biochemistry 36 10 1685 1698 |
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Open Polar |
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CentAUR: Central Archive at the University of Reading |
op_collection_id |
ftunivreading |
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description |
This study quantifies the influence of Poa alpina on the soil microbial community in primary succession of alpine ecosystems, and whether these effects are controlled by the successional stage. Four successional sites representative of four stages of grassland development (initial, 4 years (non-vegetated); pioneer, 20 years; transition, 75 years; mature, 9500 years old) on the Rotmoos glacier foreland, Austria, were sampled. The size, composition and activity of the microbial community in the rhizosphere and bulk soil were characterized using the chloroform-fumigation extraction procedure, phospholipid fatty acid (PLFA) analysis and measurements of the enzymes beta-glucosidase, beta-xylosidase, N-acetyl-beta-glucosaminidase, leucine aminopeptidase, acid phosphatase and sulfatase. The interplay between the host plant and the successional stage was quantified using principal component (PCA) and multidimensional scaling analyses. Correlation analyses were applied to evaluate the relationship between soil factors (C-org, N-t, C/N ratio, pH, ammonium, phosphorus, potassium) and microbial properties in the bulk soil. In the pioneer stage microbial colonization of the rhizosphere of P. alpina was dependent on the reservoir of microbial species in the bulk soil. As a consequence, the rhizosphere and bulk soil were similar in microbial biomass (ninhydrin-reactive nitrogen (NHR-N)), community composition (PLFA), and enzyme activity. In the transition and mature grassland stage, more benign soil conditions stimulated microbial growth (NHR-N, total amount of PLFA, bacterial PLFA, Gram-positive bacteria, Gram-negative bacteria), and microbial diversity (Shannon index H) in the rhizosphere either directly or indirectly through enhanced carbon allocation. In the same period, the rhizosphere microflora shifted from a G(-) to a more G(+), and from a fungal to a more bacteria-dominated community. Rhizosphere beta-xylosidase, N-acetyl-beta-glucosaminidase, and sulfatase activity peaked in the mature grassland soil, whereas ... |
format |
Conference Object |
author |
Tscherko, D. Hammesfahr, U. Marx, M. C. Kandeler, E. |
spellingShingle |
Tscherko, D. Hammesfahr, U. Marx, M. C. Kandeler, E. Shifts in rhizosphere microbial communities and enzyme activity of Poa alpina across an alpine chronosequence |
author_facet |
Tscherko, D. Hammesfahr, U. Marx, M. C. Kandeler, E. |
author_sort |
Tscherko, D. |
title |
Shifts in rhizosphere microbial communities and enzyme activity of Poa alpina across an alpine chronosequence |
title_short |
Shifts in rhizosphere microbial communities and enzyme activity of Poa alpina across an alpine chronosequence |
title_full |
Shifts in rhizosphere microbial communities and enzyme activity of Poa alpina across an alpine chronosequence |
title_fullStr |
Shifts in rhizosphere microbial communities and enzyme activity of Poa alpina across an alpine chronosequence |
title_full_unstemmed |
Shifts in rhizosphere microbial communities and enzyme activity of Poa alpina across an alpine chronosequence |
title_sort |
shifts in rhizosphere microbial communities and enzyme activity of poa alpina across an alpine chronosequence |
publisher |
Pergamon-Elsevier Science Ltd |
publishDate |
2003 |
url |
https://centaur.reading.ac.uk/4160/ |
genre |
Poa alpina |
genre_facet |
Poa alpina |
op_relation |
Tscherko, D., Hammesfahr, U., Marx, M. C. and Kandeler, E. (2003) Shifts in rhizosphere microbial communities and enzyme activity of Poa alpina across an alpine chronosequence. In: 2nd Enzymes in the Environment Conference, Prague, CZECH REPUBLIC, pp. 1685-1698. doi: https://doi.org/10.1016/j.soilbio.2004.07.004 <https://doi.org/10.1016/j.soilbio.2004.07.004> |
op_doi |
https://doi.org/10.1016/j.soilbio.2004.07.004 |
container_title |
Soil Biology and Biochemistry |
container_volume |
36 |
container_issue |
10 |
container_start_page |
1685 |
op_container_end_page |
1698 |
_version_ |
1776203226227408896 |