The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation
Background The myotome is the primitive skeletal muscle that forms within the embryonic metameric body wall. It can be subdivided into an epaxial and hypaxial domain. It has been shown that the formation of the epaxial myotome requires the dorsomedial lip of the dermomyotome (DML). Although the vent...
Published in: | BMC Developmental Biology |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BioMed Central
2013
|
Subjects: | |
Online Access: | https://centaur.reading.ac.uk/35941/ https://centaur.reading.ac.uk/35941/1/1471-213X-13-37.pdf https://doi.org/10.1186/1471-213X-13-37 |
id |
ftunivreading:oai:centaur.reading.ac.uk:35941 |
---|---|
record_format |
openpolar |
spelling |
ftunivreading:oai:centaur.reading.ac.uk:35941 2024-06-23T07:52:23+00:00 The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation Pu, Qin Abduelmula, Aisha Masyuk, Maryna Theiss, Carsten Schwandulla, Dieter Hans, Michael Patel, Ketan Brand-Saberi, Beate Huang, Ruijin 2013 text https://centaur.reading.ac.uk/35941/ https://centaur.reading.ac.uk/35941/1/1471-213X-13-37.pdf https://doi.org/10.1186/1471-213X-13-37 en eng BioMed Central https://centaur.reading.ac.uk/35941/1/1471-213X-13-37.pdf Pu, Q., Abduelmula, A., Masyuk, M., Theiss, C., Schwandulla, D., Hans, M., Patel, K. <https://centaur.reading.ac.uk/view/creators/90000575.html>, Brand-Saberi, B. and Huang, R. (2013) The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation. BMC Developmental Biology, 13 (1). 37. ISSN 1471-213X doi: https://doi.org/10.1186/1471-213X-13-37 <https://doi.org/10.1186/1471-213X-13-37> cc_by Article PeerReviewed 2013 ftunivreading https://doi.org/10.1186/1471-213X-13-37 2024-06-11T15:01:09Z Background The myotome is the primitive skeletal muscle that forms within the embryonic metameric body wall. It can be subdivided into an epaxial and hypaxial domain. It has been shown that the formation of the epaxial myotome requires the dorsomedial lip of the dermomyotome (DML). Although the ventrolateral lip (VLL) of the dermomyotome is believed to be required for the formation of the hypaxial myotome, experimentally evidence for this statement still needs to be provided. Provision of such data would enable the resolution of a debate regarding the formation of the hypaxial dermomyotome. Two mechanisms have been proposed for this tissue. The first proposes that the intermediate dermomyotome undergoes cellular expansion thereby pushing the ventral lateral lip in a lateral direction (translocation). In contrast, the alternative view holds that the ventral lateral lip grows laterally. Results Using time lapse confocal microscopy, we observed that the GFP-labelled ventrolateral lip (VLL) of the dermomyotome grows rather than translocates in a lateral direction. The necessity of the VLL for lateral extension of the myotome was addressed by ablation studies. We found that the hypaxial myotome did not form after VLL ablation. In contrast, the removal of an intermediate portion of the dermomyotome had very little effect of the hypaxial myotome. These results demonstrate that the VLL is required for the formation of the hypaxial myotome. Conclusion Our study demonstrates that the dermomyotome ventrolateral lip is essential for the hypaxial myotome formation and supports the lip extension model. Therefore, despite being under independent signalling controls, both the dorsomedial and ventrolateral lip fulfil the same function, i.e. they extend into adjacent regions permitting the growth of the myotome. Article in Journal/Newspaper DML CentAUR: Central Archive at the University of Reading BMC Developmental Biology 13 1 37 |
institution |
Open Polar |
collection |
CentAUR: Central Archive at the University of Reading |
op_collection_id |
ftunivreading |
language |
English |
description |
Background The myotome is the primitive skeletal muscle that forms within the embryonic metameric body wall. It can be subdivided into an epaxial and hypaxial domain. It has been shown that the formation of the epaxial myotome requires the dorsomedial lip of the dermomyotome (DML). Although the ventrolateral lip (VLL) of the dermomyotome is believed to be required for the formation of the hypaxial myotome, experimentally evidence for this statement still needs to be provided. Provision of such data would enable the resolution of a debate regarding the formation of the hypaxial dermomyotome. Two mechanisms have been proposed for this tissue. The first proposes that the intermediate dermomyotome undergoes cellular expansion thereby pushing the ventral lateral lip in a lateral direction (translocation). In contrast, the alternative view holds that the ventral lateral lip grows laterally. Results Using time lapse confocal microscopy, we observed that the GFP-labelled ventrolateral lip (VLL) of the dermomyotome grows rather than translocates in a lateral direction. The necessity of the VLL for lateral extension of the myotome was addressed by ablation studies. We found that the hypaxial myotome did not form after VLL ablation. In contrast, the removal of an intermediate portion of the dermomyotome had very little effect of the hypaxial myotome. These results demonstrate that the VLL is required for the formation of the hypaxial myotome. Conclusion Our study demonstrates that the dermomyotome ventrolateral lip is essential for the hypaxial myotome formation and supports the lip extension model. Therefore, despite being under independent signalling controls, both the dorsomedial and ventrolateral lip fulfil the same function, i.e. they extend into adjacent regions permitting the growth of the myotome. |
format |
Article in Journal/Newspaper |
author |
Pu, Qin Abduelmula, Aisha Masyuk, Maryna Theiss, Carsten Schwandulla, Dieter Hans, Michael Patel, Ketan Brand-Saberi, Beate Huang, Ruijin |
spellingShingle |
Pu, Qin Abduelmula, Aisha Masyuk, Maryna Theiss, Carsten Schwandulla, Dieter Hans, Michael Patel, Ketan Brand-Saberi, Beate Huang, Ruijin The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
author_facet |
Pu, Qin Abduelmula, Aisha Masyuk, Maryna Theiss, Carsten Schwandulla, Dieter Hans, Michael Patel, Ketan Brand-Saberi, Beate Huang, Ruijin |
author_sort |
Pu, Qin |
title |
The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
title_short |
The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
title_full |
The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
title_fullStr |
The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
title_full_unstemmed |
The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
title_sort |
dermomyotome ventrolateral lip is essential for the hypaxial myotome formation |
publisher |
BioMed Central |
publishDate |
2013 |
url |
https://centaur.reading.ac.uk/35941/ https://centaur.reading.ac.uk/35941/1/1471-213X-13-37.pdf https://doi.org/10.1186/1471-213X-13-37 |
genre |
DML |
genre_facet |
DML |
op_relation |
https://centaur.reading.ac.uk/35941/1/1471-213X-13-37.pdf Pu, Q., Abduelmula, A., Masyuk, M., Theiss, C., Schwandulla, D., Hans, M., Patel, K. <https://centaur.reading.ac.uk/view/creators/90000575.html>, Brand-Saberi, B. and Huang, R. (2013) The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation. BMC Developmental Biology, 13 (1). 37. ISSN 1471-213X doi: https://doi.org/10.1186/1471-213X-13-37 <https://doi.org/10.1186/1471-213X-13-37> |
op_rights |
cc_by |
op_doi |
https://doi.org/10.1186/1471-213X-13-37 |
container_title |
BMC Developmental Biology |
container_volume |
13 |
container_issue |
1 |
container_start_page |
37 |
_version_ |
1802643677001023488 |