Methanotrophy across a natural permafrost thaw environment
The fate of carbon sequestered in permafrost is a key concern for future global warming as this large carbon stock is rapidly becoming a net methane source due to widespread thaw. Methane release from permafrost is moderated by methanotrophs, which oxidise 20-60% of this methane before emission to t...
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2018
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:cf80af7 2023-05-15T12:59:41+02:00 Methanotrophy across a natural permafrost thaw environment Singleton, Caitlin M. McCalley, Carmody K. Woodcroft, Ben J. Boyd, Joel A. Evans, Paul N. Hodgkins, Suzanne B. Chanton, Jeffrey P. Frolking, Steve Crill, Patrick M. Saleska, Scott R. Rich, Virginia I. Tyson, Gene W. 2018-06-28 https://espace.library.uq.edu.au/view/UQ:cf80af7 eng eng Nature Publishing Group doi:10.1038/s41396-018-0065-5 issn:1751-7370 issn:1751-7362 orcid:0000-0003-0670-7480 orcid:0000-0001-5995-2602 orcid:0000-0001-8559-9427 DE-SC0004632 DE-SC0010580 DESC0016440 Not set Ecology Evolution Behavior and Systematics Microbiology 1105 Ecology 2404 Microbiology Journal Article 2018 ftunivqespace https://doi.org/10.1038/s41396-018-0065-5 2020-12-29T00:46:15Z The fate of carbon sequestered in permafrost is a key concern for future global warming as this large carbon stock is rapidly becoming a net methane source due to widespread thaw. Methane release from permafrost is moderated by methanotrophs, which oxidise 20-60% of this methane before emission to the atmosphere. Despite the importance of methanotrophs to carbon cycling, these microorganisms are under-characterised and have not been studied across a natural permafrost thaw gradient. Here, we examine methanotroph communities from the active layer of a permafrost thaw gradient in Stordalen Mire (Abisko, Sweden) spanning three years, analysing 188 metagenomes and 24 metatranscriptomes paired with in situ biogeochemical data. Methanotroph community composition and activity varied significantly as thaw progressed from intact permafrost palsa, to partially thawed bog and fully thawed fen. Thirteen methanotroph population genomes were recovered, including two novel genomes belonging to the uncultivated upland soil cluster alpha (USCα) group and a novel potentially methanotrophic Hyphomicrobiaceae. Combined analysis of porewater δC-CH isotopes and methanotroph abundances showed methane oxidation was greatest below the oxic-anoxic interface in the bog. These results detail the direct effect of thaw on autochthonous methanotroph communities, and their consequent changes in population structure, activity and methane moderation potential. Article in Journal/Newspaper Abisko palsa permafrost The University of Queensland: UQ eSpace Abisko ENVELOPE(18.829,18.829,68.349,68.349) Stordalen ENVELOPE(7.337,7.337,62.510,62.510) The ISME Journal 12 10 2544 2558 |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Ecology Evolution Behavior and Systematics Microbiology 1105 Ecology 2404 Microbiology |
spellingShingle |
Ecology Evolution Behavior and Systematics Microbiology 1105 Ecology 2404 Microbiology Singleton, Caitlin M. McCalley, Carmody K. Woodcroft, Ben J. Boyd, Joel A. Evans, Paul N. Hodgkins, Suzanne B. Chanton, Jeffrey P. Frolking, Steve Crill, Patrick M. Saleska, Scott R. Rich, Virginia I. Tyson, Gene W. Methanotrophy across a natural permafrost thaw environment |
topic_facet |
Ecology Evolution Behavior and Systematics Microbiology 1105 Ecology 2404 Microbiology |
description |
The fate of carbon sequestered in permafrost is a key concern for future global warming as this large carbon stock is rapidly becoming a net methane source due to widespread thaw. Methane release from permafrost is moderated by methanotrophs, which oxidise 20-60% of this methane before emission to the atmosphere. Despite the importance of methanotrophs to carbon cycling, these microorganisms are under-characterised and have not been studied across a natural permafrost thaw gradient. Here, we examine methanotroph communities from the active layer of a permafrost thaw gradient in Stordalen Mire (Abisko, Sweden) spanning three years, analysing 188 metagenomes and 24 metatranscriptomes paired with in situ biogeochemical data. Methanotroph community composition and activity varied significantly as thaw progressed from intact permafrost palsa, to partially thawed bog and fully thawed fen. Thirteen methanotroph population genomes were recovered, including two novel genomes belonging to the uncultivated upland soil cluster alpha (USCα) group and a novel potentially methanotrophic Hyphomicrobiaceae. Combined analysis of porewater δC-CH isotopes and methanotroph abundances showed methane oxidation was greatest below the oxic-anoxic interface in the bog. These results detail the direct effect of thaw on autochthonous methanotroph communities, and their consequent changes in population structure, activity and methane moderation potential. |
format |
Article in Journal/Newspaper |
author |
Singleton, Caitlin M. McCalley, Carmody K. Woodcroft, Ben J. Boyd, Joel A. Evans, Paul N. Hodgkins, Suzanne B. Chanton, Jeffrey P. Frolking, Steve Crill, Patrick M. Saleska, Scott R. Rich, Virginia I. Tyson, Gene W. |
author_facet |
Singleton, Caitlin M. McCalley, Carmody K. Woodcroft, Ben J. Boyd, Joel A. Evans, Paul N. Hodgkins, Suzanne B. Chanton, Jeffrey P. Frolking, Steve Crill, Patrick M. Saleska, Scott R. Rich, Virginia I. Tyson, Gene W. |
author_sort |
Singleton, Caitlin M. |
title |
Methanotrophy across a natural permafrost thaw environment |
title_short |
Methanotrophy across a natural permafrost thaw environment |
title_full |
Methanotrophy across a natural permafrost thaw environment |
title_fullStr |
Methanotrophy across a natural permafrost thaw environment |
title_full_unstemmed |
Methanotrophy across a natural permafrost thaw environment |
title_sort |
methanotrophy across a natural permafrost thaw environment |
publisher |
Nature Publishing Group |
publishDate |
2018 |
url |
https://espace.library.uq.edu.au/view/UQ:cf80af7 |
long_lat |
ENVELOPE(18.829,18.829,68.349,68.349) ENVELOPE(7.337,7.337,62.510,62.510) |
geographic |
Abisko Stordalen |
geographic_facet |
Abisko Stordalen |
genre |
Abisko palsa permafrost |
genre_facet |
Abisko palsa permafrost |
op_relation |
doi:10.1038/s41396-018-0065-5 issn:1751-7370 issn:1751-7362 orcid:0000-0003-0670-7480 orcid:0000-0001-5995-2602 orcid:0000-0001-8559-9427 DE-SC0004632 DE-SC0010580 DESC0016440 Not set |
op_doi |
https://doi.org/10.1038/s41396-018-0065-5 |
container_title |
The ISME Journal |
container_volume |
12 |
container_issue |
10 |
container_start_page |
2544 |
op_container_end_page |
2558 |
_version_ |
1766077283867557888 |