Genomic and metabolomic analysis of Antarctic bacteria revealed culture and elicitation conditions for the production of antimicrobial compounds
Concern about finding new antibiotics against drug-resistant pathogens is increasing every year. Antarctic bacteria have been proposed as an unexplored source of bioactive metabolites; however, most biosynthetic gene clusters (BGCs) producing secondary metabolites remain silent under common culture...
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:b5627e8 2023-05-15T13:42:01+02:00 Genomic and metabolomic analysis of Antarctic bacteria revealed culture and elicitation conditions for the production of antimicrobial compounds Núñez-Montero, Kattia Quezada-Solís, Damián Khalil, Zeinab G. Capon, Robert J. Andreote, Fernando D. Barrientos, Leticia 2020-04-27 https://espace.library.uq.edu.au/view/UQ:b5627e8 eng eng MDPI doi:10.3390/biom10050673 issn:2218-273X orcid:0000-0002-9285-1720 orcid:0000-0002-8341-7754 INACH RT_14-12 DG_01-19 DI19-0079 NXR17-0003 CONICYT-PFCHA/Doctorado Nacional/2017-21170263 antibiotics discovery cold-adapted bacteria genome mining metabolomics secondary metabolites silent gene clusters 1303 Biochemistry 1312 Molecular Biology Journal Article 2020 ftunivqespace https://doi.org/10.3390/biom10050673 2020-12-22T15:09:03Z Concern about finding new antibiotics against drug-resistant pathogens is increasing every year. Antarctic bacteria have been proposed as an unexplored source of bioactive metabolites; however, most biosynthetic gene clusters (BGCs) producing secondary metabolites remain silent under common culture conditions. Our work aimed to characterize elicitation conditions for the production of antibacterial secondary metabolites from 34 Antarctic bacterial strains based on MS/MS metabolomics and genome mining approaches. Bacterial strains were cultivated under different nutrient and elicitation conditions, including the addition of lipopolysaccharide (LPS), sodium nitroprusside (SNP), and coculture. Metabolomes were obtained by HPLC-QTOF-MS/MS and analyzed through molecular networking. Antibacterial activity was determined, and seven strains were selected for genome sequencing and analysis. Biosynthesis pathways were activated by all the elicitation treatments, which varies among strains and dependents of culture media. Increased antibacterial activity was observed for a few strains and addition of LPS was related with inhibition of Gram-negative pathogens. Antibiotic BGCs were found for all selected strains and the expressions of putative actinomycin, carotenoids, and bacillibactin were characterized by comparison of genomic and metabolomic data. This work established the use of promising new elicitors for bioprospection of Antarctic bacteria and highlights the importance of new "-omics" comparative approaches for drug discovery. Article in Journal/Newspaper Antarc* Antarctic The University of Queensland: UQ eSpace Antarctic Biomolecules 10 5 673 |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
antibiotics discovery cold-adapted bacteria genome mining metabolomics secondary metabolites silent gene clusters 1303 Biochemistry 1312 Molecular Biology |
spellingShingle |
antibiotics discovery cold-adapted bacteria genome mining metabolomics secondary metabolites silent gene clusters 1303 Biochemistry 1312 Molecular Biology Núñez-Montero, Kattia Quezada-Solís, Damián Khalil, Zeinab G. Capon, Robert J. Andreote, Fernando D. Barrientos, Leticia Genomic and metabolomic analysis of Antarctic bacteria revealed culture and elicitation conditions for the production of antimicrobial compounds |
topic_facet |
antibiotics discovery cold-adapted bacteria genome mining metabolomics secondary metabolites silent gene clusters 1303 Biochemistry 1312 Molecular Biology |
description |
Concern about finding new antibiotics against drug-resistant pathogens is increasing every year. Antarctic bacteria have been proposed as an unexplored source of bioactive metabolites; however, most biosynthetic gene clusters (BGCs) producing secondary metabolites remain silent under common culture conditions. Our work aimed to characterize elicitation conditions for the production of antibacterial secondary metabolites from 34 Antarctic bacterial strains based on MS/MS metabolomics and genome mining approaches. Bacterial strains were cultivated under different nutrient and elicitation conditions, including the addition of lipopolysaccharide (LPS), sodium nitroprusside (SNP), and coculture. Metabolomes were obtained by HPLC-QTOF-MS/MS and analyzed through molecular networking. Antibacterial activity was determined, and seven strains were selected for genome sequencing and analysis. Biosynthesis pathways were activated by all the elicitation treatments, which varies among strains and dependents of culture media. Increased antibacterial activity was observed for a few strains and addition of LPS was related with inhibition of Gram-negative pathogens. Antibiotic BGCs were found for all selected strains and the expressions of putative actinomycin, carotenoids, and bacillibactin were characterized by comparison of genomic and metabolomic data. This work established the use of promising new elicitors for bioprospection of Antarctic bacteria and highlights the importance of new "-omics" comparative approaches for drug discovery. |
format |
Article in Journal/Newspaper |
author |
Núñez-Montero, Kattia Quezada-Solís, Damián Khalil, Zeinab G. Capon, Robert J. Andreote, Fernando D. Barrientos, Leticia |
author_facet |
Núñez-Montero, Kattia Quezada-Solís, Damián Khalil, Zeinab G. Capon, Robert J. Andreote, Fernando D. Barrientos, Leticia |
author_sort |
Núñez-Montero, Kattia |
title |
Genomic and metabolomic analysis of Antarctic bacteria revealed culture and elicitation conditions for the production of antimicrobial compounds |
title_short |
Genomic and metabolomic analysis of Antarctic bacteria revealed culture and elicitation conditions for the production of antimicrobial compounds |
title_full |
Genomic and metabolomic analysis of Antarctic bacteria revealed culture and elicitation conditions for the production of antimicrobial compounds |
title_fullStr |
Genomic and metabolomic analysis of Antarctic bacteria revealed culture and elicitation conditions for the production of antimicrobial compounds |
title_full_unstemmed |
Genomic and metabolomic analysis of Antarctic bacteria revealed culture and elicitation conditions for the production of antimicrobial compounds |
title_sort |
genomic and metabolomic analysis of antarctic bacteria revealed culture and elicitation conditions for the production of antimicrobial compounds |
publisher |
MDPI |
publishDate |
2020 |
url |
https://espace.library.uq.edu.au/view/UQ:b5627e8 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
doi:10.3390/biom10050673 issn:2218-273X orcid:0000-0002-9285-1720 orcid:0000-0002-8341-7754 INACH RT_14-12 DG_01-19 DI19-0079 NXR17-0003 CONICYT-PFCHA/Doctorado Nacional/2017-21170263 |
op_doi |
https://doi.org/10.3390/biom10050673 |
container_title |
Biomolecules |
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10 |
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5 |
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673 |
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1766161837302218752 |