Genetic tagging of free-ranging black and brown bears
Identification of individuals in a free-ranging animal population is potentially hampered by a lack of distinguishing features (e.g., scars, unique color patterns), poor visibility (e.g., densely forested environments), cost and invasiveness of physical capture, and mark loss. Advances in DNA-analys...
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:9d631d8 2023-05-15T18:41:56+02:00 Genetic tagging of free-ranging black and brown bears Woods, John G. Paetkau, David Lewis, David McLellan, Bruce N. Proctor, Michael Strobeck, Curtis 1999-09-01 https://espace.library.uq.edu.au/view/UQ:9d631d8 eng eng issn:0091-7648 Black bear Brown bear DNA Hair Mark recapture Microsatellite Ursus americanus Ursus arctos 2309 Nature and Landscape Conservation Journal Article 1999 ftunivqespace 2020-12-29T00:43:02Z Identification of individuals in a free-ranging animal population is potentially hampered by a lack of distinguishing features (e.g., scars, unique color patterns), poor visibility (e.g., densely forested environments), cost and invasiveness of physical capture, and mark loss. Advances in DNA-analysis technology offer alternative methods of individual identification that may overcome several of these problems. We investigated the genetic variability of American black bears (Ursus americanus) and brown (grizzly) bears (Ursus arctos) in the Columbia River basin of British Columbia, Canada, and developed a method to obtain genetic samples from free-ranging bears. We established the background genetic variability using microsatellite genotyping at 9 loci using tissue and blood samples from captured bears. In 3 field trials, we tested methods to obtain hair from free-ranging bears. Although all methods collected hair suitable for DNA analysis, the barbed-wire enclosure hair-trap was superior. We extracted DNA from hair roots and identified sample species with a species-specific mitochondrial DNA (mtDNA) test and sample sex from a Y-chromosome test. Using 6 microsatellite loci from nuclear DNA (nDNA), we screened all hair samples for individual identity and developed match probability functions based on scenarios of random sampling (P(random)), the likely presence of parent-offspring groupings in the samples (P(par-offs)), and the likely presence of siblings in the samples (P(sib)). We applied the P(sib) to each hair sample (match criteria at P(sib) Article in Journal/Newspaper Ursus arctos The University of Queensland: UQ eSpace Canada British Columbia ENVELOPE(-125.003,-125.003,54.000,54.000) |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Black bear Brown bear DNA Hair Mark recapture Microsatellite Ursus americanus Ursus arctos 2309 Nature and Landscape Conservation |
spellingShingle |
Black bear Brown bear DNA Hair Mark recapture Microsatellite Ursus americanus Ursus arctos 2309 Nature and Landscape Conservation Woods, John G. Paetkau, David Lewis, David McLellan, Bruce N. Proctor, Michael Strobeck, Curtis Genetic tagging of free-ranging black and brown bears |
topic_facet |
Black bear Brown bear DNA Hair Mark recapture Microsatellite Ursus americanus Ursus arctos 2309 Nature and Landscape Conservation |
description |
Identification of individuals in a free-ranging animal population is potentially hampered by a lack of distinguishing features (e.g., scars, unique color patterns), poor visibility (e.g., densely forested environments), cost and invasiveness of physical capture, and mark loss. Advances in DNA-analysis technology offer alternative methods of individual identification that may overcome several of these problems. We investigated the genetic variability of American black bears (Ursus americanus) and brown (grizzly) bears (Ursus arctos) in the Columbia River basin of British Columbia, Canada, and developed a method to obtain genetic samples from free-ranging bears. We established the background genetic variability using microsatellite genotyping at 9 loci using tissue and blood samples from captured bears. In 3 field trials, we tested methods to obtain hair from free-ranging bears. Although all methods collected hair suitable for DNA analysis, the barbed-wire enclosure hair-trap was superior. We extracted DNA from hair roots and identified sample species with a species-specific mitochondrial DNA (mtDNA) test and sample sex from a Y-chromosome test. Using 6 microsatellite loci from nuclear DNA (nDNA), we screened all hair samples for individual identity and developed match probability functions based on scenarios of random sampling (P(random)), the likely presence of parent-offspring groupings in the samples (P(par-offs)), and the likely presence of siblings in the samples (P(sib)). We applied the P(sib) to each hair sample (match criteria at P(sib) |
format |
Article in Journal/Newspaper |
author |
Woods, John G. Paetkau, David Lewis, David McLellan, Bruce N. Proctor, Michael Strobeck, Curtis |
author_facet |
Woods, John G. Paetkau, David Lewis, David McLellan, Bruce N. Proctor, Michael Strobeck, Curtis |
author_sort |
Woods, John G. |
title |
Genetic tagging of free-ranging black and brown bears |
title_short |
Genetic tagging of free-ranging black and brown bears |
title_full |
Genetic tagging of free-ranging black and brown bears |
title_fullStr |
Genetic tagging of free-ranging black and brown bears |
title_full_unstemmed |
Genetic tagging of free-ranging black and brown bears |
title_sort |
genetic tagging of free-ranging black and brown bears |
publishDate |
1999 |
url |
https://espace.library.uq.edu.au/view/UQ:9d631d8 |
long_lat |
ENVELOPE(-125.003,-125.003,54.000,54.000) |
geographic |
Canada British Columbia |
geographic_facet |
Canada British Columbia |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_relation |
issn:0091-7648 |
_version_ |
1766231508703510528 |