The effectiveness of heat, cold and 6-dimethylaminopurine shocks for inducing tetraploidy in the Kuruma shrimp, Marsupenaeus japonicus (Bate)
In this study tetraploid Marsupenaeus japonicus (Bate) embryos were produced by preventing the first division in mitosis. The effectiveness of temperature and chemical shocks for producing tetraploid M. japonicus were assessed when applied at different times postspawning and for different durations....
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National Shellfisheries Association
2006
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:81043 2023-05-15T15:59:09+02:00 The effectiveness of heat, cold and 6-dimethylaminopurine shocks for inducing tetraploidy in the Kuruma shrimp, Marsupenaeus japonicus (Bate) Sellars, M. J. Coman, F. E. Degnan, B. M. Preston, N. P. 2006-01-01 https://espace.library.uq.edu.au/view/UQ:81043/thumbnail_UQ81043_OA_t.jpg https://espace.library.uq.edu.au/view/UQ:81043/UQ81043_OA.pdf https://espace.library.uq.edu.au/view/UQ:81043 eng eng National Shellfisheries Association doi:10.2983/0730-8000(2006)25[631:TEOHCA]2.0.CO;2 issn:0730-8000 issn:1943-6319 orcid:0000-0001-7573-8518 Penaeus Japonicus Polyploidy Shrimp Selective Breeding Genetic Protection Fisheries Marine & Freshwater Biology Crassostrea-gigas Thunberg Pacific Oyster Induction Carp Embryos Eggs 270805 Genetic Engineering and Enzyme Technology C1 630303 Aquaculture Journal Article 2006 ftunivqespace https://doi.org/10.2983/0730-8000(2006)25[631:TEOHCA]2.0.CO;2 2020-08-24T22:38:51Z In this study tetraploid Marsupenaeus japonicus (Bate) embryos were produced by preventing the first division in mitosis. The effectiveness of temperature and chemical shocks for producing tetraploid M. japonicus were assessed when applied at different times postspawning and for different durations. Tetraploid M. japonicus embryos (spawned at 27 degrees C) were produced by heat shocks at 35 degrees C and 36 degrees C in three and eight spawning samples respectively, and a cold shock at 5 degrees C in a single spawning sample. All temperature shocks inducing tetraploidy were applied 18-23 min postspawning for a 5-10 min duration. The percentage of spawnings successfully inducing tetraploid embryos (i.e., frequency of induction) ranged from 33.33% to 66.67% for the 21, 22 and 23 min postspawning heat shock treatment regimes. The percentage of tetraploid embryos within an induction (i.e., induction rate), as determined by flow cytometry, ranged from 8.82% to 98.12% (ave. S.E.) (34.4 +/- 21.4%) for the 35 degrees C shock treatments, from 13.12% to 61.02% (35.0 +/- 5.0%) for the 36 degrees C shock treatments and was 15% for the 5 degrees C cold shock treatment. No tetraploids were produced for spawnings that received heat shocks above 36 degrees C or below 35 degrees C, or for cold shocks above 5 degrees C for any of the tested postspawning treatment and duration times. Chemical shock with 150 mu M 6-dimethylaminopurine did not result in tetraploid M. japonicus embryos at any of the tested postspawning treatment times and durations. Tetraploid M. japonicus embryos were nonviable, with no tetraploid larvae being detected by flow cytometry. Based on our results heat shocking of M. japonicus embryos at 36 degrees C, 23 min postspawning for a 5-10 min duration is the most effective means to produce tetraploids through inhibition of the first mitotic division (taking into consideration the importance of frequency and induction rate equally). Article in Journal/Newspaper Crassostrea gigas Pacific oyster The University of Queensland: UQ eSpace Pacific |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Penaeus Japonicus Polyploidy Shrimp Selective Breeding Genetic Protection Fisheries Marine & Freshwater Biology Crassostrea-gigas Thunberg Pacific Oyster Induction Carp Embryos Eggs 270805 Genetic Engineering and Enzyme Technology C1 630303 Aquaculture |
spellingShingle |
Penaeus Japonicus Polyploidy Shrimp Selective Breeding Genetic Protection Fisheries Marine & Freshwater Biology Crassostrea-gigas Thunberg Pacific Oyster Induction Carp Embryos Eggs 270805 Genetic Engineering and Enzyme Technology C1 630303 Aquaculture Sellars, M. J. Coman, F. E. Degnan, B. M. Preston, N. P. The effectiveness of heat, cold and 6-dimethylaminopurine shocks for inducing tetraploidy in the Kuruma shrimp, Marsupenaeus japonicus (Bate) |
topic_facet |
Penaeus Japonicus Polyploidy Shrimp Selective Breeding Genetic Protection Fisheries Marine & Freshwater Biology Crassostrea-gigas Thunberg Pacific Oyster Induction Carp Embryos Eggs 270805 Genetic Engineering and Enzyme Technology C1 630303 Aquaculture |
description |
In this study tetraploid Marsupenaeus japonicus (Bate) embryos were produced by preventing the first division in mitosis. The effectiveness of temperature and chemical shocks for producing tetraploid M. japonicus were assessed when applied at different times postspawning and for different durations. Tetraploid M. japonicus embryos (spawned at 27 degrees C) were produced by heat shocks at 35 degrees C and 36 degrees C in three and eight spawning samples respectively, and a cold shock at 5 degrees C in a single spawning sample. All temperature shocks inducing tetraploidy were applied 18-23 min postspawning for a 5-10 min duration. The percentage of spawnings successfully inducing tetraploid embryos (i.e., frequency of induction) ranged from 33.33% to 66.67% for the 21, 22 and 23 min postspawning heat shock treatment regimes. The percentage of tetraploid embryos within an induction (i.e., induction rate), as determined by flow cytometry, ranged from 8.82% to 98.12% (ave. S.E.) (34.4 +/- 21.4%) for the 35 degrees C shock treatments, from 13.12% to 61.02% (35.0 +/- 5.0%) for the 36 degrees C shock treatments and was 15% for the 5 degrees C cold shock treatment. No tetraploids were produced for spawnings that received heat shocks above 36 degrees C or below 35 degrees C, or for cold shocks above 5 degrees C for any of the tested postspawning treatment and duration times. Chemical shock with 150 mu M 6-dimethylaminopurine did not result in tetraploid M. japonicus embryos at any of the tested postspawning treatment times and durations. Tetraploid M. japonicus embryos were nonviable, with no tetraploid larvae being detected by flow cytometry. Based on our results heat shocking of M. japonicus embryos at 36 degrees C, 23 min postspawning for a 5-10 min duration is the most effective means to produce tetraploids through inhibition of the first mitotic division (taking into consideration the importance of frequency and induction rate equally). |
format |
Article in Journal/Newspaper |
author |
Sellars, M. J. Coman, F. E. Degnan, B. M. Preston, N. P. |
author_facet |
Sellars, M. J. Coman, F. E. Degnan, B. M. Preston, N. P. |
author_sort |
Sellars, M. J. |
title |
The effectiveness of heat, cold and 6-dimethylaminopurine shocks for inducing tetraploidy in the Kuruma shrimp, Marsupenaeus japonicus (Bate) |
title_short |
The effectiveness of heat, cold and 6-dimethylaminopurine shocks for inducing tetraploidy in the Kuruma shrimp, Marsupenaeus japonicus (Bate) |
title_full |
The effectiveness of heat, cold and 6-dimethylaminopurine shocks for inducing tetraploidy in the Kuruma shrimp, Marsupenaeus japonicus (Bate) |
title_fullStr |
The effectiveness of heat, cold and 6-dimethylaminopurine shocks for inducing tetraploidy in the Kuruma shrimp, Marsupenaeus japonicus (Bate) |
title_full_unstemmed |
The effectiveness of heat, cold and 6-dimethylaminopurine shocks for inducing tetraploidy in the Kuruma shrimp, Marsupenaeus japonicus (Bate) |
title_sort |
effectiveness of heat, cold and 6-dimethylaminopurine shocks for inducing tetraploidy in the kuruma shrimp, marsupenaeus japonicus (bate) |
publisher |
National Shellfisheries Association |
publishDate |
2006 |
url |
https://espace.library.uq.edu.au/view/UQ:81043/thumbnail_UQ81043_OA_t.jpg https://espace.library.uq.edu.au/view/UQ:81043/UQ81043_OA.pdf https://espace.library.uq.edu.au/view/UQ:81043 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.2983/0730-8000(2006)25[631:TEOHCA]2.0.CO;2 issn:0730-8000 issn:1943-6319 orcid:0000-0001-7573-8518 |
op_doi |
https://doi.org/10.2983/0730-8000(2006)25[631:TEOHCA]2.0.CO;2 |
_version_ |
1766394935694589952 |