Phosphate uptake and growth kinetics of Trichodesmium (Cyanobacteria) isolates from the North Atlantic Ocean and the Great Barrier Reef, Australia
We compared inorganic phosphate (P-i) uptake and growth kinetics of two cultures of the diazotrophic cyanobacterium Trichodesmium isolated from the North Atlantic Ocean (IMS101) and from the Great Barrier Reef, Australia (GBRTRLI101). Phosphate-limited cultures had up to six times higher maximum P-i...
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Language: | English |
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Blackwell Publishing Inc
2005
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:76316 2023-05-15T17:30:10+02:00 Phosphate uptake and growth kinetics of Trichodesmium (Cyanobacteria) isolates from the North Atlantic Ocean and the Great Barrier Reef, Australia Fu, Fei-Xue Zhang, Yaohong Bell, Peter R. F. Hutchins, David A. Patricia A Wheeler 2005-01-01 https://espace.library.uq.edu.au/view/UQ:76316 eng eng Blackwell Publishing Inc doi:10.1111/j.1529-8817.2005.04063.x issn:0022-3646 Plant Sciences Marine & Freshwater Biology Cyanobacteria P Limitation Phosphate Uptake Redfield Ratio Trichodesmium Pacific Subtropical Gyre Nitrogen-fixation Marine Cyanobacterium Natural-populations Continuous Culture N-2 Fixation Phosphorus Lagoon Spp Phytoplankton 290600 Chemical Engineering C1 670799 Other Journal Article 2005 ftunivqespace https://doi.org/10.1111/j.1529-8817.2005.04063.x 2020-09-21T22:32:26Z We compared inorganic phosphate (P-i) uptake and growth kinetics of two cultures of the diazotrophic cyanobacterium Trichodesmium isolated from the North Atlantic Ocean (IMS101) and from the Great Barrier Reef, Australia (GBRTRLI101). Phosphate-limited cultures had up to six times higher maximum P-i uptake rates than P-replete cultures in both strains. For strain GBRTRLI101, cell-specific P-i uptake rates were nearly twice as high, due to larger cell size, but P-specific maximum uptake rates were similar for both isolates. Half saturation constants were 0.4 and 0.6 muM for P-i uptake and 0.1 and 0.2 muM for growth in IMS101 and GBRTRLI101, respectively. Phosphate uptake in both strains was correlated to growth rates rather than to light or temperature. The cellular phosphorus quota for both strains increased with increasing P-i up to 1.0 muM. The C:P ratios were 340-390 and N:P ratios were 40-45 for both strains under severely P-limited growth conditions, similar to reported values for natural populations from the tropical Atlantic and Pacific Oceans. The C:P and N:P ratios were near Redfield values in medium with >1.0 muM P-i. The North Atlantic strain IMS101 is better adapted to growing on P-i at low concentrations than is GBRTRLI101 from the more P-i-enriched Great Barrier Reef. However, neither strain can achieve appreciable growth at the very low (nanomolar) P-i concentrations found in most oligotrophic regimes. Phosphate could be an important source of phosphorus for Trichodesmium on the Great Barrier Reef, but populations growing in the oligotrophic open ocean must rely primarily on dissolved organic phosphorus sources. Article in Journal/Newspaper North Atlantic The University of Queensland: UQ eSpace Pacific Journal of Phycology 41 1 62 73 |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Plant Sciences Marine & Freshwater Biology Cyanobacteria P Limitation Phosphate Uptake Redfield Ratio Trichodesmium Pacific Subtropical Gyre Nitrogen-fixation Marine Cyanobacterium Natural-populations Continuous Culture N-2 Fixation Phosphorus Lagoon Spp Phytoplankton 290600 Chemical Engineering C1 670799 Other |
spellingShingle |
Plant Sciences Marine & Freshwater Biology Cyanobacteria P Limitation Phosphate Uptake Redfield Ratio Trichodesmium Pacific Subtropical Gyre Nitrogen-fixation Marine Cyanobacterium Natural-populations Continuous Culture N-2 Fixation Phosphorus Lagoon Spp Phytoplankton 290600 Chemical Engineering C1 670799 Other Fu, Fei-Xue Zhang, Yaohong Bell, Peter R. F. Hutchins, David A. Phosphate uptake and growth kinetics of Trichodesmium (Cyanobacteria) isolates from the North Atlantic Ocean and the Great Barrier Reef, Australia |
topic_facet |
Plant Sciences Marine & Freshwater Biology Cyanobacteria P Limitation Phosphate Uptake Redfield Ratio Trichodesmium Pacific Subtropical Gyre Nitrogen-fixation Marine Cyanobacterium Natural-populations Continuous Culture N-2 Fixation Phosphorus Lagoon Spp Phytoplankton 290600 Chemical Engineering C1 670799 Other |
description |
We compared inorganic phosphate (P-i) uptake and growth kinetics of two cultures of the diazotrophic cyanobacterium Trichodesmium isolated from the North Atlantic Ocean (IMS101) and from the Great Barrier Reef, Australia (GBRTRLI101). Phosphate-limited cultures had up to six times higher maximum P-i uptake rates than P-replete cultures in both strains. For strain GBRTRLI101, cell-specific P-i uptake rates were nearly twice as high, due to larger cell size, but P-specific maximum uptake rates were similar for both isolates. Half saturation constants were 0.4 and 0.6 muM for P-i uptake and 0.1 and 0.2 muM for growth in IMS101 and GBRTRLI101, respectively. Phosphate uptake in both strains was correlated to growth rates rather than to light or temperature. The cellular phosphorus quota for both strains increased with increasing P-i up to 1.0 muM. The C:P ratios were 340-390 and N:P ratios were 40-45 for both strains under severely P-limited growth conditions, similar to reported values for natural populations from the tropical Atlantic and Pacific Oceans. The C:P and N:P ratios were near Redfield values in medium with >1.0 muM P-i. The North Atlantic strain IMS101 is better adapted to growing on P-i at low concentrations than is GBRTRLI101 from the more P-i-enriched Great Barrier Reef. However, neither strain can achieve appreciable growth at the very low (nanomolar) P-i concentrations found in most oligotrophic regimes. Phosphate could be an important source of phosphorus for Trichodesmium on the Great Barrier Reef, but populations growing in the oligotrophic open ocean must rely primarily on dissolved organic phosphorus sources. |
author2 |
Patricia A Wheeler |
format |
Article in Journal/Newspaper |
author |
Fu, Fei-Xue Zhang, Yaohong Bell, Peter R. F. Hutchins, David A. |
author_facet |
Fu, Fei-Xue Zhang, Yaohong Bell, Peter R. F. Hutchins, David A. |
author_sort |
Fu, Fei-Xue |
title |
Phosphate uptake and growth kinetics of Trichodesmium (Cyanobacteria) isolates from the North Atlantic Ocean and the Great Barrier Reef, Australia |
title_short |
Phosphate uptake and growth kinetics of Trichodesmium (Cyanobacteria) isolates from the North Atlantic Ocean and the Great Barrier Reef, Australia |
title_full |
Phosphate uptake and growth kinetics of Trichodesmium (Cyanobacteria) isolates from the North Atlantic Ocean and the Great Barrier Reef, Australia |
title_fullStr |
Phosphate uptake and growth kinetics of Trichodesmium (Cyanobacteria) isolates from the North Atlantic Ocean and the Great Barrier Reef, Australia |
title_full_unstemmed |
Phosphate uptake and growth kinetics of Trichodesmium (Cyanobacteria) isolates from the North Atlantic Ocean and the Great Barrier Reef, Australia |
title_sort |
phosphate uptake and growth kinetics of trichodesmium (cyanobacteria) isolates from the north atlantic ocean and the great barrier reef, australia |
publisher |
Blackwell Publishing Inc |
publishDate |
2005 |
url |
https://espace.library.uq.edu.au/view/UQ:76316 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
doi:10.1111/j.1529-8817.2005.04063.x issn:0022-3646 |
op_doi |
https://doi.org/10.1111/j.1529-8817.2005.04063.x |
container_title |
Journal of Phycology |
container_volume |
41 |
container_issue |
1 |
container_start_page |
62 |
op_container_end_page |
73 |
_version_ |
1766125991245119488 |