Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi
Halohasta litchfieldiae represents ∼ 44% and Halorubrum lacusprofundi ∼ 10% of the hypersaline, perennially cold (≥ −20°C) Deep Lake community in Antarctica. We used proteomics and microscopy to define physiological responses of these haloarchaea to growth at high (30°C) and low (10 and 4°C) tempera...
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:716002 2023-05-15T13:52:34+02:00 Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi Williams, Timothy J. Liao, Yan Ye, Jun Kuchel, Rhiannon P. Poljak, Anne Raftery, Mark J. Cavicchioli, Ricardo 2017-06-01 https://espace.library.uq.edu.au/view/UQ:716002 eng eng Wiley-Blackwell Publishing doi:10.1111/1462-2920.13705 issn:1462-2920 issn:1462-2912 orcid:0000-0002-6476-6406 DP150100244 201206910027 201206230085 Ecology Evolution Behavior and Systematics Microbiology 1105 Ecology 2404 Microbiology Journal Article 2017 ftunivqespace https://doi.org/10.1111/1462-2920.13705 2020-12-08T02:55:52Z Halohasta litchfieldiae represents ∼ 44% and Halorubrum lacusprofundi ∼ 10% of the hypersaline, perennially cold (≥ −20°C) Deep Lake community in Antarctica. We used proteomics and microscopy to define physiological responses of these haloarchaea to growth at high (30°C) and low (10 and 4°C) temperatures. The proteomic data indicate that both species responded to low temperature by modifying their cell envelope including protein N-glycosylation, maintaining osmotic balance and translation initiation, and modifying RNA turnover and tRNA modification. Distinctions between the two species included DNA protection and repair strategies (e.g. roles of UspA and Rad50), and metabolism of glycerol and pyruvate. For Hrr. lacusprofundi, low temperature led to the formation of polyhydroxyalkanoate-like granules, with granule formation occurring by an unknown mechanism. Hrr. lacusprofundi also formed biofilms and synthesized high levels of Hsp20 chaperones. Hht. litchfieldiae was characterized by an active CRISPR system, and elevated levels of the core gene expression machinery, which contrasted markedly to the decreased levels of Hrr. lacusprofundi. These findings greatly expand the understanding of cellular mechanisms of cold adaptation in psychrophilic archaea, and provide insight into how Hht. litchfieldiae gains dominance in Deep Lake. Article in Journal/Newspaper Antarc* Antarctic Antarctica The University of Queensland: UQ eSpace Antarctic The Antarctic Environmental Microbiology 19 6 2210 2227 |
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Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Ecology Evolution Behavior and Systematics Microbiology 1105 Ecology 2404 Microbiology |
spellingShingle |
Ecology Evolution Behavior and Systematics Microbiology 1105 Ecology 2404 Microbiology Williams, Timothy J. Liao, Yan Ye, Jun Kuchel, Rhiannon P. Poljak, Anne Raftery, Mark J. Cavicchioli, Ricardo Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi |
topic_facet |
Ecology Evolution Behavior and Systematics Microbiology 1105 Ecology 2404 Microbiology |
description |
Halohasta litchfieldiae represents ∼ 44% and Halorubrum lacusprofundi ∼ 10% of the hypersaline, perennially cold (≥ −20°C) Deep Lake community in Antarctica. We used proteomics and microscopy to define physiological responses of these haloarchaea to growth at high (30°C) and low (10 and 4°C) temperatures. The proteomic data indicate that both species responded to low temperature by modifying their cell envelope including protein N-glycosylation, maintaining osmotic balance and translation initiation, and modifying RNA turnover and tRNA modification. Distinctions between the two species included DNA protection and repair strategies (e.g. roles of UspA and Rad50), and metabolism of glycerol and pyruvate. For Hrr. lacusprofundi, low temperature led to the formation of polyhydroxyalkanoate-like granules, with granule formation occurring by an unknown mechanism. Hrr. lacusprofundi also formed biofilms and synthesized high levels of Hsp20 chaperones. Hht. litchfieldiae was characterized by an active CRISPR system, and elevated levels of the core gene expression machinery, which contrasted markedly to the decreased levels of Hrr. lacusprofundi. These findings greatly expand the understanding of cellular mechanisms of cold adaptation in psychrophilic archaea, and provide insight into how Hht. litchfieldiae gains dominance in Deep Lake. |
format |
Article in Journal/Newspaper |
author |
Williams, Timothy J. Liao, Yan Ye, Jun Kuchel, Rhiannon P. Poljak, Anne Raftery, Mark J. Cavicchioli, Ricardo |
author_facet |
Williams, Timothy J. Liao, Yan Ye, Jun Kuchel, Rhiannon P. Poljak, Anne Raftery, Mark J. Cavicchioli, Ricardo |
author_sort |
Williams, Timothy J. |
title |
Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi |
title_short |
Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi |
title_full |
Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi |
title_fullStr |
Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi |
title_full_unstemmed |
Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi |
title_sort |
cold adaptation of the antarctic haloarchaea halohasta litchfieldiae and halorubrum lacusprofundi |
publisher |
Wiley-Blackwell Publishing |
publishDate |
2017 |
url |
https://espace.library.uq.edu.au/view/UQ:716002 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
doi:10.1111/1462-2920.13705 issn:1462-2920 issn:1462-2912 orcid:0000-0002-6476-6406 DP150100244 201206910027 201206230085 |
op_doi |
https://doi.org/10.1111/1462-2920.13705 |
container_title |
Environmental Microbiology |
container_volume |
19 |
container_issue |
6 |
container_start_page |
2210 |
op_container_end_page |
2227 |
_version_ |
1766256990946852864 |