Neoparamoeba perurans loses virulence during clonal culture
Amoebic Gill Disease affects farmed salmonids and is caused by Neoparamoeba perurans. Clonal cultures of this amoeba have been used for challenge experiments, however the effect of long-term culture on virulence has not been investigated. Here we show, using in vitro and in vivo methods, that a clon...
Published in: | International Journal for Parasitology |
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2015
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Online Access: | https://espace.library.uq.edu.au/view/UQ:7098b21 |
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:7098b21 2023-05-15T15:32:14+02:00 Neoparamoeba perurans loses virulence during clonal culture Bridle, Andrew R. Davenport, Danielle L. Crosbie, Philip B. B. Polinski, Mark Nowak, Barbara F. 2015-08-01 https://espace.library.uq.edu.au/view/UQ:7098b21 eng eng Elsevier doi:10.1016/j.ijpara.2015.04.005 issn:0020-7519 orcid:0000-0002-9252-4404 Amebic Gill Disease Atlantic Salmon Naegleria-Fowleri Acanthamoeba Salar Infection Journal Article 2015 ftunivqespace https://doi.org/10.1016/j.ijpara.2015.04.005 2020-12-29T01:10:48Z Amoebic Gill Disease affects farmed salmonids and is caused by Neoparamoeba perurans. Clonal cultures of this amoeba have been used for challenge experiments, however the effect of long-term culture on virulence has not been investigated. Here we show, using in vitro and in vivo methods, that a clone of N. perurans which was virulent 70 days after clonal culture lost virulence after 3 years in clonal culture. We propose that this is related either to the lack of attachment to the gills or the absence of an extracellular product, as shown by the lack of cytopathic effect on Chinook salmon embryo cells. The avirulent clonal culture of N. perurans allowed us to propose two potential virulence mechanisms/factors involved in Amoebic Gill Disease and is an invaluable tool for host-pathogen studies of Amoebic Gill Disease. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Atlantic salmon The University of Queensland: UQ eSpace International Journal for Parasitology 45 9-10 575 578 |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Amebic Gill Disease Atlantic Salmon Naegleria-Fowleri Acanthamoeba Salar Infection |
spellingShingle |
Amebic Gill Disease Atlantic Salmon Naegleria-Fowleri Acanthamoeba Salar Infection Bridle, Andrew R. Davenport, Danielle L. Crosbie, Philip B. B. Polinski, Mark Nowak, Barbara F. Neoparamoeba perurans loses virulence during clonal culture |
topic_facet |
Amebic Gill Disease Atlantic Salmon Naegleria-Fowleri Acanthamoeba Salar Infection |
description |
Amoebic Gill Disease affects farmed salmonids and is caused by Neoparamoeba perurans. Clonal cultures of this amoeba have been used for challenge experiments, however the effect of long-term culture on virulence has not been investigated. Here we show, using in vitro and in vivo methods, that a clone of N. perurans which was virulent 70 days after clonal culture lost virulence after 3 years in clonal culture. We propose that this is related either to the lack of attachment to the gills or the absence of an extracellular product, as shown by the lack of cytopathic effect on Chinook salmon embryo cells. The avirulent clonal culture of N. perurans allowed us to propose two potential virulence mechanisms/factors involved in Amoebic Gill Disease and is an invaluable tool for host-pathogen studies of Amoebic Gill Disease. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Bridle, Andrew R. Davenport, Danielle L. Crosbie, Philip B. B. Polinski, Mark Nowak, Barbara F. |
author_facet |
Bridle, Andrew R. Davenport, Danielle L. Crosbie, Philip B. B. Polinski, Mark Nowak, Barbara F. |
author_sort |
Bridle, Andrew R. |
title |
Neoparamoeba perurans loses virulence during clonal culture |
title_short |
Neoparamoeba perurans loses virulence during clonal culture |
title_full |
Neoparamoeba perurans loses virulence during clonal culture |
title_fullStr |
Neoparamoeba perurans loses virulence during clonal culture |
title_full_unstemmed |
Neoparamoeba perurans loses virulence during clonal culture |
title_sort |
neoparamoeba perurans loses virulence during clonal culture |
publisher |
Elsevier |
publishDate |
2015 |
url |
https://espace.library.uq.edu.au/view/UQ:7098b21 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
doi:10.1016/j.ijpara.2015.04.005 issn:0020-7519 orcid:0000-0002-9252-4404 |
op_doi |
https://doi.org/10.1016/j.ijpara.2015.04.005 |
container_title |
International Journal for Parasitology |
container_volume |
45 |
container_issue |
9-10 |
container_start_page |
575 |
op_container_end_page |
578 |
_version_ |
1766362746329235456 |