Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi
Biofilms enhance rates of gene exchange, access to specific nutrients, and cell survivability. Haloarchaea in Deep Lake, Antarctica, are characterized by high rates of intergenera gene exchange, metabolic specialization that promotes niche adaptation, and are exposed to high levels of UV-irradiation...
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:6e93d42 2023-05-15T13:59:08+02:00 Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi Liao, Y. Williams, T. J. Ye, J. Charlesworth, J. Burns, B. P. Poljak, A. Raftery, M. J. Cavicchioli, R. 2016-11-22 https://espace.library.uq.edu.au/view/UQ:6e93d42 eng eng Nature Publishing Group doi:10.1038/srep37454 issn:2045-2322 orcid:0000-0002-6476-6406 DP150100244 201206910027 Bacillus-Sphaericus Nctc-9602 Oxidative Stress Conditions Thin-Layer-Chromatography Horizontal Gene-Transfer Extracellular Dna Escherichia-Coli Microbial Biofilms Bacterial Biofilms Growth-Rate Deep Lake Journal Article 2016 ftunivqespace https://doi.org/10.1038/srep37454 2020-11-17T05:30:25Z Biofilms enhance rates of gene exchange, access to specific nutrients, and cell survivability. Haloarchaea in Deep Lake, Antarctica, are characterized by high rates of intergenera gene exchange, metabolic specialization that promotes niche adaptation, and are exposed to high levels of UV-irradiation in summer. Halorubrum lacusprofundi from Deep Lake has previously been reported to form biofilms. Here we defined growth conditions that promoted the formation of biofilms and used microscopy and enzymatic digestion of extracellular material to characterize biofilm structures. Extracellular DNA was found to be critical to biofilms, with cell surface proteins and quorum sensing also implicated in biofilm formation. Quantitative proteomics was used to define pathways and cellular processes involved in forming biofilms; these included enhanced purine synthesis and specific cell surface proteins involved in DNA metabolism; post-translational modification of cell surface proteins; specific pathways of carbon metabolism involving acetyl-CoA; and specific responses to oxidative stress. The study provides a new level of understanding about the molecular mechanisms involved in biofilm formation of this important member of the Deep Lake community. Article in Journal/Newspaper Antarc* Antarctic Antarctica The University of Queensland: UQ eSpace Antarctic The Antarctic Scientific Reports 6 1 |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Bacillus-Sphaericus Nctc-9602 Oxidative Stress Conditions Thin-Layer-Chromatography Horizontal Gene-Transfer Extracellular Dna Escherichia-Coli Microbial Biofilms Bacterial Biofilms Growth-Rate Deep Lake |
spellingShingle |
Bacillus-Sphaericus Nctc-9602 Oxidative Stress Conditions Thin-Layer-Chromatography Horizontal Gene-Transfer Extracellular Dna Escherichia-Coli Microbial Biofilms Bacterial Biofilms Growth-Rate Deep Lake Liao, Y. Williams, T. J. Ye, J. Charlesworth, J. Burns, B. P. Poljak, A. Raftery, M. J. Cavicchioli, R. Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi |
topic_facet |
Bacillus-Sphaericus Nctc-9602 Oxidative Stress Conditions Thin-Layer-Chromatography Horizontal Gene-Transfer Extracellular Dna Escherichia-Coli Microbial Biofilms Bacterial Biofilms Growth-Rate Deep Lake |
description |
Biofilms enhance rates of gene exchange, access to specific nutrients, and cell survivability. Haloarchaea in Deep Lake, Antarctica, are characterized by high rates of intergenera gene exchange, metabolic specialization that promotes niche adaptation, and are exposed to high levels of UV-irradiation in summer. Halorubrum lacusprofundi from Deep Lake has previously been reported to form biofilms. Here we defined growth conditions that promoted the formation of biofilms and used microscopy and enzymatic digestion of extracellular material to characterize biofilm structures. Extracellular DNA was found to be critical to biofilms, with cell surface proteins and quorum sensing also implicated in biofilm formation. Quantitative proteomics was used to define pathways and cellular processes involved in forming biofilms; these included enhanced purine synthesis and specific cell surface proteins involved in DNA metabolism; post-translational modification of cell surface proteins; specific pathways of carbon metabolism involving acetyl-CoA; and specific responses to oxidative stress. The study provides a new level of understanding about the molecular mechanisms involved in biofilm formation of this important member of the Deep Lake community. |
format |
Article in Journal/Newspaper |
author |
Liao, Y. Williams, T. J. Ye, J. Charlesworth, J. Burns, B. P. Poljak, A. Raftery, M. J. Cavicchioli, R. |
author_facet |
Liao, Y. Williams, T. J. Ye, J. Charlesworth, J. Burns, B. P. Poljak, A. Raftery, M. J. Cavicchioli, R. |
author_sort |
Liao, Y. |
title |
Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi |
title_short |
Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi |
title_full |
Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi |
title_fullStr |
Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi |
title_full_unstemmed |
Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi |
title_sort |
morphological and proteomic analysis of biofilms from the antarctic archaeon, halorubrum lacusprofundi |
publisher |
Nature Publishing Group |
publishDate |
2016 |
url |
https://espace.library.uq.edu.au/view/UQ:6e93d42 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
doi:10.1038/srep37454 issn:2045-2322 orcid:0000-0002-6476-6406 DP150100244 201206910027 |
op_doi |
https://doi.org/10.1038/srep37454 |
container_title |
Scientific Reports |
container_volume |
6 |
container_issue |
1 |
_version_ |
1766267551477661696 |