Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon
A genomics approach was used to identify nutritionally regulated genes involved in growth of fast skeletal muscle in Atlantic salmon (Salmo salar L.). Forward and reverse subtractive cDNA libraries were prepared comparing fish with zero growth rates to fish growing rapidly. We produced 7,420 ESTs an...
Published in: | Physiological Genomics |
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Language: | English |
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American Physiological Society
2010
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:5e8ccd2 2023-05-15T15:31:53+02:00 Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon Bower, Neil I. Johnston, Ian A. 2010-09-01 https://espace.library.uq.edu.au/view/UQ:5e8ccd2 eng eng American Physiological Society doi:10.1152/physiolgenomics.00065.2010 issn:1094-8341 issn:1531-2267 orcid:0000-0002-6764-6063 Fasting Heat shock proteins Myogenesis Myogenic cells Nutrition Refeeding 1311 Genetics 1314 Physiology Journal Article 2010 ftunivqespace https://doi.org/10.1152/physiolgenomics.00065.2010 2020-12-08T04:55:05Z A genomics approach was used to identify nutritionally regulated genes involved in growth of fast skeletal muscle in Atlantic salmon (Salmo salar L.). Forward and reverse subtractive cDNA libraries were prepared comparing fish with zero growth rates to fish growing rapidly. We produced 7,420 ESTs and assembled them into nonredundant clusters prior to annotation. Contigs representing 40 potentially unrecognized nutritionally responsive candidate genes were identified. Twenty-three of the subtractive library candidates were also differentially regulated by nutritional state in an independent fasting-refeeding experiment and their expression placed in the context of 26 genes with established roles in muscle growth regulation. The expression of these genes was also determined during the maturation of a primary myocyte culture, identifying 13 candidates from the subtractive cDNA libraries with putative roles in the myogenic program. During early stages of refeeding DNAJA4, HSPA1B, HSP90A, and CHAC1 expression increased, indicating activation of unfolded protein response pathways. Four genes were considered inhibitory to myogenesis based on their in vivo and in vitro expression profiles (CEBPD, ASB2, HSP30, novel transcript GE623928). Other genes showed increased expression with feeding and highest in vitro expression during the proliferative phase of the culture (FOXD1, DRG1) or as cells differentiated (SMYD1, RTN1, MID1IP1, HSP90A, novel transcript GE617747). The genes identified were associated with chromatin modification (SMYD1, RTN1), microtubule stabilization (MID1IP1), cell cycle regulation (FOXD1, CEBPD, DRG1), and negative regulation of signaling (ASB2) and may play a role in the stimulation of myogenesis during the transition from a catabolic to anabolic state in skeletal muscle. Article in Journal/Newspaper Atlantic salmon Salmo salar The University of Queensland: UQ eSpace Physiological Genomics 42A 2 114 130 |
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Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Fasting Heat shock proteins Myogenesis Myogenic cells Nutrition Refeeding 1311 Genetics 1314 Physiology |
spellingShingle |
Fasting Heat shock proteins Myogenesis Myogenic cells Nutrition Refeeding 1311 Genetics 1314 Physiology Bower, Neil I. Johnston, Ian A. Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon |
topic_facet |
Fasting Heat shock proteins Myogenesis Myogenic cells Nutrition Refeeding 1311 Genetics 1314 Physiology |
description |
A genomics approach was used to identify nutritionally regulated genes involved in growth of fast skeletal muscle in Atlantic salmon (Salmo salar L.). Forward and reverse subtractive cDNA libraries were prepared comparing fish with zero growth rates to fish growing rapidly. We produced 7,420 ESTs and assembled them into nonredundant clusters prior to annotation. Contigs representing 40 potentially unrecognized nutritionally responsive candidate genes were identified. Twenty-three of the subtractive library candidates were also differentially regulated by nutritional state in an independent fasting-refeeding experiment and their expression placed in the context of 26 genes with established roles in muscle growth regulation. The expression of these genes was also determined during the maturation of a primary myocyte culture, identifying 13 candidates from the subtractive cDNA libraries with putative roles in the myogenic program. During early stages of refeeding DNAJA4, HSPA1B, HSP90A, and CHAC1 expression increased, indicating activation of unfolded protein response pathways. Four genes were considered inhibitory to myogenesis based on their in vivo and in vitro expression profiles (CEBPD, ASB2, HSP30, novel transcript GE623928). Other genes showed increased expression with feeding and highest in vitro expression during the proliferative phase of the culture (FOXD1, DRG1) or as cells differentiated (SMYD1, RTN1, MID1IP1, HSP90A, novel transcript GE617747). The genes identified were associated with chromatin modification (SMYD1, RTN1), microtubule stabilization (MID1IP1), cell cycle regulation (FOXD1, CEBPD, DRG1), and negative regulation of signaling (ASB2) and may play a role in the stimulation of myogenesis during the transition from a catabolic to anabolic state in skeletal muscle. |
format |
Article in Journal/Newspaper |
author |
Bower, Neil I. Johnston, Ian A. |
author_facet |
Bower, Neil I. Johnston, Ian A. |
author_sort |
Bower, Neil I. |
title |
Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon |
title_short |
Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon |
title_full |
Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon |
title_fullStr |
Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon |
title_full_unstemmed |
Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon |
title_sort |
discovery and characterization of nutritionally regulated genes associated with muscle growth in atlantic salmon |
publisher |
American Physiological Society |
publishDate |
2010 |
url |
https://espace.library.uq.edu.au/view/UQ:5e8ccd2 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.1152/physiolgenomics.00065.2010 issn:1094-8341 issn:1531-2267 orcid:0000-0002-6764-6063 |
op_doi |
https://doi.org/10.1152/physiolgenomics.00065.2010 |
container_title |
Physiological Genomics |
container_volume |
42A |
container_issue |
2 |
container_start_page |
114 |
op_container_end_page |
130 |
_version_ |
1766362384432103424 |