A hydrogel-based enzyme-loaded polymersome reactor
In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs un...
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Royal Society of Chemistry
2010
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:596255 2023-05-15T13:59:53+02:00 A hydrogel-based enzyme-loaded polymersome reactor De Hoog, Hans-Peter M. Arends, Isabel W. C. E. Rowan, Alan E. Cornelissen, Jeroen J. L. M. Nolte, Roeland J. M. 2010-05-01 https://espace.library.uq.edu.au/view/UQ:596255 eng eng Royal Society of Chemistry doi:10.1039/b9nr00325h issn:2040-3364 issn:2040-3372 orcid:0000-0003-0728-3851 Chemistry Multidisciplinary Nanoscience & Nanotechnology Materials Science Physics Applied Science & Technology - Other Topics 2500 Materials Science Journal Article 2010 ftunivqespace https://doi.org/10.1039/b9nr00325h 2020-10-27T03:53:45Z In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs under mild conditions and does not inhibit the activity of the encapsulated enzymes nor does it affect the structure of the polymersomes. The stability of the polymersome hydrogel architecture is demonstrated by the facile recycling of the polymersomes and their use in repeated reaction cycles. A 'continuous-flow polymersome reactor' is constructed in which substrate is added to the top of the reactor and product is collected at the bottom. This set-up allows the use of different enzymes and the processing of multiple substrates, as is demonstrated by the conversion of 2-methoxyphenyl acetate to tetraguaiacol in a reactor loaded with polymersome hydrogels containing the enzymes Candida antarctica lipase B (CALB) and glucose oxidase (GOx). Article in Journal/Newspaper Antarc* Antarctica The University of Queensland: UQ eSpace Nanoscale 2 5 709 |
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Open Polar |
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The University of Queensland: UQ eSpace |
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ftunivqespace |
language |
English |
topic |
Chemistry Multidisciplinary Nanoscience & Nanotechnology Materials Science Physics Applied Science & Technology - Other Topics 2500 Materials Science |
spellingShingle |
Chemistry Multidisciplinary Nanoscience & Nanotechnology Materials Science Physics Applied Science & Technology - Other Topics 2500 Materials Science De Hoog, Hans-Peter M. Arends, Isabel W. C. E. Rowan, Alan E. Cornelissen, Jeroen J. L. M. Nolte, Roeland J. M. A hydrogel-based enzyme-loaded polymersome reactor |
topic_facet |
Chemistry Multidisciplinary Nanoscience & Nanotechnology Materials Science Physics Applied Science & Technology - Other Topics 2500 Materials Science |
description |
In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs under mild conditions and does not inhibit the activity of the encapsulated enzymes nor does it affect the structure of the polymersomes. The stability of the polymersome hydrogel architecture is demonstrated by the facile recycling of the polymersomes and their use in repeated reaction cycles. A 'continuous-flow polymersome reactor' is constructed in which substrate is added to the top of the reactor and product is collected at the bottom. This set-up allows the use of different enzymes and the processing of multiple substrates, as is demonstrated by the conversion of 2-methoxyphenyl acetate to tetraguaiacol in a reactor loaded with polymersome hydrogels containing the enzymes Candida antarctica lipase B (CALB) and glucose oxidase (GOx). |
format |
Article in Journal/Newspaper |
author |
De Hoog, Hans-Peter M. Arends, Isabel W. C. E. Rowan, Alan E. Cornelissen, Jeroen J. L. M. Nolte, Roeland J. M. |
author_facet |
De Hoog, Hans-Peter M. Arends, Isabel W. C. E. Rowan, Alan E. Cornelissen, Jeroen J. L. M. Nolte, Roeland J. M. |
author_sort |
De Hoog, Hans-Peter M. |
title |
A hydrogel-based enzyme-loaded polymersome reactor |
title_short |
A hydrogel-based enzyme-loaded polymersome reactor |
title_full |
A hydrogel-based enzyme-loaded polymersome reactor |
title_fullStr |
A hydrogel-based enzyme-loaded polymersome reactor |
title_full_unstemmed |
A hydrogel-based enzyme-loaded polymersome reactor |
title_sort |
hydrogel-based enzyme-loaded polymersome reactor |
publisher |
Royal Society of Chemistry |
publishDate |
2010 |
url |
https://espace.library.uq.edu.au/view/UQ:596255 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
doi:10.1039/b9nr00325h issn:2040-3364 issn:2040-3372 orcid:0000-0003-0728-3851 |
op_doi |
https://doi.org/10.1039/b9nr00325h |
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Nanoscale |
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2 |
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5 |
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709 |
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1766268806915686400 |