Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by Ostreid herpes virus 1(mu)var under experimental challenge may be mediated by humoral antiviral activity
Ostreid herpesvirus I (OsHV-1) has induced mass mortalities of the larvae and spat of Pacific oysters, Crassostrea gigas, in Europe and, more recently, in Oceania. The production of pearls from the Black-lip pearl oyster, Pinctada margaritifera, represents the second largest source of income to the...
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2015
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:352542 2023-05-15T15:59:08+02:00 Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by Ostreid herpes virus 1(mu)var under experimental challenge may be mediated by humoral antiviral activity Tan, Terence L. S. Paul-Pont, Ika Evans, Olivia M. Watterson, Daniel Young, Paul Whittington, Richard Fougerouse, Angelique Bichet, Hervé Barnes, Andrew C. Dang, Cécile 2015-02-21 https://espace.library.uq.edu.au/view/UQ:352542 eng eng Academic Press doi:10.1016/j.fsi.2015.02.026 issn:1050-4648 issn:1095-9947 orcid:0000-0002-2040-5190 orcid:0000-0002-3990-8070 AGROBIOSPHERE ANR-11-AGRO-006 Not set Ostreid herpesvirus Humoral immunity Hemocytes Pinctada Herpes 1104 Aquatic Science 2304 Environmental Chemistry Journal Article 2015 ftunivqespace https://doi.org/10.1016/j.fsi.2015.02.026 2020-11-24T00:40:10Z Ostreid herpesvirus I (OsHV-1) has induced mass mortalities of the larvae and spat of Pacific oysters, Crassostrea gigas, in Europe and, more recently, in Oceania. The production of pearls from the Black-lip pearl oyster, Pinctada margaritifera, represents the second largest source of income to the economies of French Polynesia and many Pacific Island nations that could be severely compromised in the event of a disease outbreak. Coincidentally with the occurrence of OsHV-1 in the southern hemisphere, C gigas imported from New Zealand and France into French Polynesia tested positive for OsHV-1. Although interspecies viral transmission has been demonstrated, the transmissibility of OsHV-1 to P. margaritifera is unknown. We investigated the susceptibility of juvenile P. margaritifera to OsHV-1 mu var that were injected with tissue homogenates sourced from either naturally infected or healthy C gigas. The infection challenge lasted 14 days post-injection (dpi) with sampling at 0, 1, 2, 3, 5, 7 and 14 days. Mortality rate, viral prevalence, and cellular immune responses in experimental animals were determined. Tissues were screened by light microscopy and TEM. Pacific oysters were also challenged and used as a positive control to validate the efficiency of OsHV-1 mu var infection. Viral particles and features such as marginated chromatin and highly electron dense nuclei were observed in C. gigas but not in P. margaritifera. Mortality rates and hemocyte immune parameters, including phagocytosis and respiratory burst, were similar between challenged and control P. margarinfera. Herpesvirus-inhibiting activity was demonstrated in the acellular fraction of the hemolymph from P. margaritifera, suggesting that the humoral immunity is critical in the defence against herpesvirus in pearl oysters. Overall, these results suggest that under the conditions of the experimental challenge, P. margaritifera was not sensitive to OsHV-1 mu var and was not an effective host/carrier. The nature and spectrum of activity of the humoral antiviral activity is worthy of further investigation. Crown Copyright (C) 2015 Published by Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas The University of Queensland: UQ eSpace Pacific New Zealand Fish & Shellfish Immunology 44 1 232 240 |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Ostreid herpesvirus Humoral immunity Hemocytes Pinctada Herpes 1104 Aquatic Science 2304 Environmental Chemistry |
spellingShingle |
Ostreid herpesvirus Humoral immunity Hemocytes Pinctada Herpes 1104 Aquatic Science 2304 Environmental Chemistry Tan, Terence L. S. Paul-Pont, Ika Evans, Olivia M. Watterson, Daniel Young, Paul Whittington, Richard Fougerouse, Angelique Bichet, Hervé Barnes, Andrew C. Dang, Cécile Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by Ostreid herpes virus 1(mu)var under experimental challenge may be mediated by humoral antiviral activity |
topic_facet |
Ostreid herpesvirus Humoral immunity Hemocytes Pinctada Herpes 1104 Aquatic Science 2304 Environmental Chemistry |
description |
Ostreid herpesvirus I (OsHV-1) has induced mass mortalities of the larvae and spat of Pacific oysters, Crassostrea gigas, in Europe and, more recently, in Oceania. The production of pearls from the Black-lip pearl oyster, Pinctada margaritifera, represents the second largest source of income to the economies of French Polynesia and many Pacific Island nations that could be severely compromised in the event of a disease outbreak. Coincidentally with the occurrence of OsHV-1 in the southern hemisphere, C gigas imported from New Zealand and France into French Polynesia tested positive for OsHV-1. Although interspecies viral transmission has been demonstrated, the transmissibility of OsHV-1 to P. margaritifera is unknown. We investigated the susceptibility of juvenile P. margaritifera to OsHV-1 mu var that were injected with tissue homogenates sourced from either naturally infected or healthy C gigas. The infection challenge lasted 14 days post-injection (dpi) with sampling at 0, 1, 2, 3, 5, 7 and 14 days. Mortality rate, viral prevalence, and cellular immune responses in experimental animals were determined. Tissues were screened by light microscopy and TEM. Pacific oysters were also challenged and used as a positive control to validate the efficiency of OsHV-1 mu var infection. Viral particles and features such as marginated chromatin and highly electron dense nuclei were observed in C. gigas but not in P. margaritifera. Mortality rates and hemocyte immune parameters, including phagocytosis and respiratory burst, were similar between challenged and control P. margarinfera. Herpesvirus-inhibiting activity was demonstrated in the acellular fraction of the hemolymph from P. margaritifera, suggesting that the humoral immunity is critical in the defence against herpesvirus in pearl oysters. Overall, these results suggest that under the conditions of the experimental challenge, P. margaritifera was not sensitive to OsHV-1 mu var and was not an effective host/carrier. The nature and spectrum of activity of the humoral antiviral activity is worthy of further investigation. Crown Copyright (C) 2015 Published by Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Tan, Terence L. S. Paul-Pont, Ika Evans, Olivia M. Watterson, Daniel Young, Paul Whittington, Richard Fougerouse, Angelique Bichet, Hervé Barnes, Andrew C. Dang, Cécile |
author_facet |
Tan, Terence L. S. Paul-Pont, Ika Evans, Olivia M. Watterson, Daniel Young, Paul Whittington, Richard Fougerouse, Angelique Bichet, Hervé Barnes, Andrew C. Dang, Cécile |
author_sort |
Tan, Terence L. S. |
title |
Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by Ostreid herpes virus 1(mu)var under experimental challenge may be mediated by humoral antiviral activity |
title_short |
Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by Ostreid herpes virus 1(mu)var under experimental challenge may be mediated by humoral antiviral activity |
title_full |
Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by Ostreid herpes virus 1(mu)var under experimental challenge may be mediated by humoral antiviral activity |
title_fullStr |
Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by Ostreid herpes virus 1(mu)var under experimental challenge may be mediated by humoral antiviral activity |
title_full_unstemmed |
Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by Ostreid herpes virus 1(mu)var under experimental challenge may be mediated by humoral antiviral activity |
title_sort |
resistance of black-lip learl oyster, pinctada margaritifera, to infection by ostreid herpes virus 1(mu)var under experimental challenge may be mediated by humoral antiviral activity |
publisher |
Academic Press |
publishDate |
2015 |
url |
https://espace.library.uq.edu.au/view/UQ:352542 |
geographic |
Pacific New Zealand |
geographic_facet |
Pacific New Zealand |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
doi:10.1016/j.fsi.2015.02.026 issn:1050-4648 issn:1095-9947 orcid:0000-0002-2040-5190 orcid:0000-0002-3990-8070 AGROBIOSPHERE ANR-11-AGRO-006 Not set |
op_doi |
https://doi.org/10.1016/j.fsi.2015.02.026 |
container_title |
Fish & Shellfish Immunology |
container_volume |
44 |
container_issue |
1 |
container_start_page |
232 |
op_container_end_page |
240 |
_version_ |
1766394918375260160 |