TLC screening of thraustochytrid strains for squalene production
Screenings of thraustochytrids (Labyrinthulomycetes) have been conducted for 176 strains isolated from various sites in the Asian region to investigate what type of species and strains accumulate high levels of squalene. Thin layer chromatography (TLC) screening for squalene production revealed that...
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:324631 2023-05-15T18:28:29+02:00 TLC screening of thraustochytrid strains for squalene production Nakazawa, Atsushi Kokubun, Yume Matsuura, Hiroshi Yonezawa, Natsuki Kose, Ryoji Yoshida, Masaki Tanabe, Yuuhiko Kusuda, Emi Van Thang, Duong Ueda, Mayumi Honda, Daiske Mahakhant, Aparat Kaya, Kunimitsu Watanabe, Makoto M. 2014-01-01 https://espace.library.uq.edu.au/view/UQ:324631 eng eng Springer doi:10.1007/s10811-013-0080-x issn:0921-8971 issn:1573-5176 Not set Aurantiochytrium Squalene Thraustochytrid TLC 1104 Aquatic Science 1110 Plant Science Journal Article 2014 ftunivqespace https://doi.org/10.1007/s10811-013-0080-x 2020-11-17T00:19:13Z Screenings of thraustochytrids (Labyrinthulomycetes) have been conducted for 176 strains isolated from various sites in the Asian region to investigate what type of species and strains accumulate high levels of squalene. Thin layer chromatography (TLC) screening for squalene production revealed that 38 strains were rated as "+" (high), 29 as "±" (medium), and 109 as "-" (low). Further, high performance liquid chromatography analysis strongly supported the TLC screening results. Besides the 18W-13a strain of Aurantiochytrium sp., which was previously recognized as a squalene-rich strain, several strains produced squalene at approximately 1 g L-1 of culture volume. Squalene production was strongly related to locality, colony color, and phylogenetic clade. Most strains with "+" squalene spots were isolated from Okinawa, a subtropical region of Japan, while the strains with "±" and "-" squalene spots were isolated from wide geographical regions from tropical to subarctic. Approximately half the strains with orange colonies on GTY medium plates produced a high amount of squalene, whereas the other strains with different colors showed less or no squalene spots on TLC. All the squalene-rich strains were assigned to the Aurantiochytrium clade. Overall, our results suggest that (1) the thraustochytrids show tendentious locality in terms of squalene production, (2) a relationship exists between the metabolic synthesis of carotenoid pigments and squalene production, and (3) the Aurantiochytrium clade may have evolved to accumulate squalene. Article in Journal/Newspaper Subarctic The University of Queensland: UQ eSpace Journal of Applied Phycology 26 1 29 41 |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Aurantiochytrium Squalene Thraustochytrid TLC 1104 Aquatic Science 1110 Plant Science |
spellingShingle |
Aurantiochytrium Squalene Thraustochytrid TLC 1104 Aquatic Science 1110 Plant Science Nakazawa, Atsushi Kokubun, Yume Matsuura, Hiroshi Yonezawa, Natsuki Kose, Ryoji Yoshida, Masaki Tanabe, Yuuhiko Kusuda, Emi Van Thang, Duong Ueda, Mayumi Honda, Daiske Mahakhant, Aparat Kaya, Kunimitsu Watanabe, Makoto M. TLC screening of thraustochytrid strains for squalene production |
topic_facet |
Aurantiochytrium Squalene Thraustochytrid TLC 1104 Aquatic Science 1110 Plant Science |
description |
Screenings of thraustochytrids (Labyrinthulomycetes) have been conducted for 176 strains isolated from various sites in the Asian region to investigate what type of species and strains accumulate high levels of squalene. Thin layer chromatography (TLC) screening for squalene production revealed that 38 strains were rated as "+" (high), 29 as "±" (medium), and 109 as "-" (low). Further, high performance liquid chromatography analysis strongly supported the TLC screening results. Besides the 18W-13a strain of Aurantiochytrium sp., which was previously recognized as a squalene-rich strain, several strains produced squalene at approximately 1 g L-1 of culture volume. Squalene production was strongly related to locality, colony color, and phylogenetic clade. Most strains with "+" squalene spots were isolated from Okinawa, a subtropical region of Japan, while the strains with "±" and "-" squalene spots were isolated from wide geographical regions from tropical to subarctic. Approximately half the strains with orange colonies on GTY medium plates produced a high amount of squalene, whereas the other strains with different colors showed less or no squalene spots on TLC. All the squalene-rich strains were assigned to the Aurantiochytrium clade. Overall, our results suggest that (1) the thraustochytrids show tendentious locality in terms of squalene production, (2) a relationship exists between the metabolic synthesis of carotenoid pigments and squalene production, and (3) the Aurantiochytrium clade may have evolved to accumulate squalene. |
format |
Article in Journal/Newspaper |
author |
Nakazawa, Atsushi Kokubun, Yume Matsuura, Hiroshi Yonezawa, Natsuki Kose, Ryoji Yoshida, Masaki Tanabe, Yuuhiko Kusuda, Emi Van Thang, Duong Ueda, Mayumi Honda, Daiske Mahakhant, Aparat Kaya, Kunimitsu Watanabe, Makoto M. |
author_facet |
Nakazawa, Atsushi Kokubun, Yume Matsuura, Hiroshi Yonezawa, Natsuki Kose, Ryoji Yoshida, Masaki Tanabe, Yuuhiko Kusuda, Emi Van Thang, Duong Ueda, Mayumi Honda, Daiske Mahakhant, Aparat Kaya, Kunimitsu Watanabe, Makoto M. |
author_sort |
Nakazawa, Atsushi |
title |
TLC screening of thraustochytrid strains for squalene production |
title_short |
TLC screening of thraustochytrid strains for squalene production |
title_full |
TLC screening of thraustochytrid strains for squalene production |
title_fullStr |
TLC screening of thraustochytrid strains for squalene production |
title_full_unstemmed |
TLC screening of thraustochytrid strains for squalene production |
title_sort |
tlc screening of thraustochytrid strains for squalene production |
publisher |
Springer |
publishDate |
2014 |
url |
https://espace.library.uq.edu.au/view/UQ:324631 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_relation |
doi:10.1007/s10811-013-0080-x issn:0921-8971 issn:1573-5176 Not set |
op_doi |
https://doi.org/10.1007/s10811-013-0080-x |
container_title |
Journal of Applied Phycology |
container_volume |
26 |
container_issue |
1 |
container_start_page |
29 |
op_container_end_page |
41 |
_version_ |
1766210981637128192 |