Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades
For over a century, venom samples from wild snakes have been collected and stored around the world. However, the quality of storage conditions for "vintage" venoms has rarely been assessed. The goal of this study was to determine whether such historical venom samples are still biochemicall...
Published in: | Journal of Proteomics |
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Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2014
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Subjects: | |
Online Access: | https://espace.library.uq.edu.au/view/UQ:323128/UQ323128OA.pdf https://espace.library.uq.edu.au/view/UQ:323128 |
id |
ftunivqespace:oai:espace.library.uq.edu.au:UQ:323128 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Venom Protein 1D gel Mass spectrometry Toxinology Stability studies 1303 Biochemistry 1304 Biophysics |
spellingShingle |
Venom Protein 1D gel Mass spectrometry Toxinology Stability studies 1303 Biochemistry 1304 Biophysics Jesupret, Clémence Baumann, Kate Jackson, Timothy N. W. Ali, Syed Abid Yang, Daryl C. Greisman, Laura Kern, Larissa Steuten, Jessica Jouiaei, Mahdokht Casewell, Nicholas R. Undheim, Eivind A. B. Koludarov, Ivan Debono, Jordan Low, Dolyce H. W. Rossi, Sarah Panagides, Nadya Winter, Kelly Ignjatovic, Vera Summerhayes, Robyn Jones, Alun Nouwens, Amanda Dunstan, Nathan Hodgson, Wayne C. Winkel, Kenneth D. Monagle, Paul Fry, Bryan Grieg Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades |
topic_facet |
Venom Protein 1D gel Mass spectrometry Toxinology Stability studies 1303 Biochemistry 1304 Biophysics |
description |
For over a century, venom samples from wild snakes have been collected and stored around the world. However, the quality of storage conditions for "vintage" venoms has rarely been assessed. The goal of this study was to determine whether such historical venom samples are still biochemically and pharmacologically viable for research purposes, or if new sample efforts are needed. In total, 52 samples spanning 5 genera and 13 species with regional variants of some species (e.g., 14 different populations of Notechis scutatus) were analysed by a combined proteomic and pharmacological approach to determine protein structural stability and bioactivity. When venoms were not exposed to air during storage, the proteomic results were virtually indistinguishable from that of fresh venom and bioactivity was equivalent or only slightly reduced. By contrast, a sample of Acanthophis antarcticus venom that was exposed to air (due to a loss of integrity of the rubber stopper) suffered significant degradation as evidenced by the proteomics profile. Interestingly, the neurotoxicity of this sample was nearly the same as fresh venom, indicating that degradation may have occurred in the free N- or C-terminus chains of the proteins, rather than at the tips of loops where the functional residues are located. These results suggest that these and other vintage venom collections may be of continuing value in toxin research. This is particularly important as many snake species worldwide are declining due to habitat destruction or modification. For some venoms (such as N. scutatus from Babel Island, Flinders Island, King Island and St. Francis Island) these were the first analyses ever conducted and these vintage samples may represent the only venom ever collected from these unique island forms of tiger snakes. Such vintage venoms may therefore represent the last remaining stocks of some local populations and thus are precious resources. These venoms also have significant historical value as the Oxyuranus venoms analysed include samples from the first coastal taipan (Oxyuranus scutellatus) collected for antivenom production (the snake that killed the collector Kevin Budden), as well as samples from the first Oxyuranus microlepidotus specimen collected after the species' rediscovery in 1976. These results demonstrate that with proper storage techniques, venom samples can retain structural and pharmacological stability. This article is part of a Special Issue entitled: Proteomics of non-model organisms. Biological significance: •These results show that with proper storage venoms are useful for decades.•These results have direct implications for the use of rare venoms. |
format |
Article in Journal/Newspaper |
author |
Jesupret, Clémence Baumann, Kate Jackson, Timothy N. W. Ali, Syed Abid Yang, Daryl C. Greisman, Laura Kern, Larissa Steuten, Jessica Jouiaei, Mahdokht Casewell, Nicholas R. Undheim, Eivind A. B. Koludarov, Ivan Debono, Jordan Low, Dolyce H. W. Rossi, Sarah Panagides, Nadya Winter, Kelly Ignjatovic, Vera Summerhayes, Robyn Jones, Alun Nouwens, Amanda Dunstan, Nathan Hodgson, Wayne C. Winkel, Kenneth D. Monagle, Paul Fry, Bryan Grieg |
author_facet |
Jesupret, Clémence Baumann, Kate Jackson, Timothy N. W. Ali, Syed Abid Yang, Daryl C. Greisman, Laura Kern, Larissa Steuten, Jessica Jouiaei, Mahdokht Casewell, Nicholas R. Undheim, Eivind A. B. Koludarov, Ivan Debono, Jordan Low, Dolyce H. W. Rossi, Sarah Panagides, Nadya Winter, Kelly Ignjatovic, Vera Summerhayes, Robyn Jones, Alun Nouwens, Amanda Dunstan, Nathan Hodgson, Wayne C. Winkel, Kenneth D. Monagle, Paul Fry, Bryan Grieg |
author_sort |
Jesupret, Clémence |
title |
Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades |
title_short |
Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades |
title_full |
Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades |
title_fullStr |
Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades |
title_full_unstemmed |
Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades |
title_sort |
vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades |
publisher |
Elsevier |
publishDate |
2014 |
url |
https://espace.library.uq.edu.au/view/UQ:323128/UQ323128OA.pdf https://espace.library.uq.edu.au/view/UQ:323128 |
long_lat |
ENVELOPE(-61.401,-61.401,-63.885,-63.885) ENVELOPE(-66.667,-66.667,-69.267,-69.267) ENVELOPE(-58.100,-58.100,-62.000,-62.000) |
geographic |
Babel Flinders King Island |
geographic_facet |
Babel Flinders King Island |
genre |
Antarc* antarcticus Francis Island |
genre_facet |
Antarc* antarcticus Francis Island |
op_relation |
doi:10.1016/j.jprot.2014.01.004 issn:1874-3919 issn:1876-7737 orcid:0000-0002-8667-3999 orcid:0000-0002-4157-5818 orcid:0000-0003-4069-8266 orcid:0000-0001-6661-1283 Not set NE/J018678/1 |
op_doi |
https://doi.org/10.1016/j.jprot.2014.01.004 |
container_title |
Journal of Proteomics |
container_volume |
105 |
container_start_page |
285 |
op_container_end_page |
294 |
_version_ |
1766251419288993792 |
spelling |
ftunivqespace:oai:espace.library.uq.edu.au:UQ:323128 2023-05-15T13:49:28+02:00 Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades Jesupret, Clémence Baumann, Kate Jackson, Timothy N. W. Ali, Syed Abid Yang, Daryl C. Greisman, Laura Kern, Larissa Steuten, Jessica Jouiaei, Mahdokht Casewell, Nicholas R. Undheim, Eivind A. B. Koludarov, Ivan Debono, Jordan Low, Dolyce H. W. Rossi, Sarah Panagides, Nadya Winter, Kelly Ignjatovic, Vera Summerhayes, Robyn Jones, Alun Nouwens, Amanda Dunstan, Nathan Hodgson, Wayne C. Winkel, Kenneth D. Monagle, Paul Fry, Bryan Grieg 2014-01-14 https://espace.library.uq.edu.au/view/UQ:323128/UQ323128OA.pdf https://espace.library.uq.edu.au/view/UQ:323128 eng eng Elsevier doi:10.1016/j.jprot.2014.01.004 issn:1874-3919 issn:1876-7737 orcid:0000-0002-8667-3999 orcid:0000-0002-4157-5818 orcid:0000-0003-4069-8266 orcid:0000-0001-6661-1283 Not set NE/J018678/1 Venom Protein 1D gel Mass spectrometry Toxinology Stability studies 1303 Biochemistry 1304 Biophysics Journal Article 2014 ftunivqespace https://doi.org/10.1016/j.jprot.2014.01.004 2020-12-15T01:06:20Z For over a century, venom samples from wild snakes have been collected and stored around the world. However, the quality of storage conditions for "vintage" venoms has rarely been assessed. The goal of this study was to determine whether such historical venom samples are still biochemically and pharmacologically viable for research purposes, or if new sample efforts are needed. In total, 52 samples spanning 5 genera and 13 species with regional variants of some species (e.g., 14 different populations of Notechis scutatus) were analysed by a combined proteomic and pharmacological approach to determine protein structural stability and bioactivity. When venoms were not exposed to air during storage, the proteomic results were virtually indistinguishable from that of fresh venom and bioactivity was equivalent or only slightly reduced. By contrast, a sample of Acanthophis antarcticus venom that was exposed to air (due to a loss of integrity of the rubber stopper) suffered significant degradation as evidenced by the proteomics profile. Interestingly, the neurotoxicity of this sample was nearly the same as fresh venom, indicating that degradation may have occurred in the free N- or C-terminus chains of the proteins, rather than at the tips of loops where the functional residues are located. These results suggest that these and other vintage venom collections may be of continuing value in toxin research. This is particularly important as many snake species worldwide are declining due to habitat destruction or modification. For some venoms (such as N. scutatus from Babel Island, Flinders Island, King Island and St. Francis Island) these were the first analyses ever conducted and these vintage samples may represent the only venom ever collected from these unique island forms of tiger snakes. Such vintage venoms may therefore represent the last remaining stocks of some local populations and thus are precious resources. These venoms also have significant historical value as the Oxyuranus venoms analysed include samples from the first coastal taipan (Oxyuranus scutellatus) collected for antivenom production (the snake that killed the collector Kevin Budden), as well as samples from the first Oxyuranus microlepidotus specimen collected after the species' rediscovery in 1976. These results demonstrate that with proper storage techniques, venom samples can retain structural and pharmacological stability. This article is part of a Special Issue entitled: Proteomics of non-model organisms. Biological significance: •These results show that with proper storage venoms are useful for decades.•These results have direct implications for the use of rare venoms. Article in Journal/Newspaper Antarc* antarcticus Francis Island The University of Queensland: UQ eSpace Babel ENVELOPE(-61.401,-61.401,-63.885,-63.885) Flinders ENVELOPE(-66.667,-66.667,-69.267,-69.267) King Island ENVELOPE(-58.100,-58.100,-62.000,-62.000) Journal of Proteomics 105 285 294 |