Postprandial expression of growth-related genes in Atlantic salmon (Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation
We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon (Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12°C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle using qu...
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Cambridge University Press
2012
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:27105dd 2023-05-15T15:31:24+02:00 Postprandial expression of growth-related genes in Atlantic salmon (Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation Valente, Luísa M.P. Bower, Neil I. Johnston, Ian A. 2012-12-28 https://espace.library.uq.edu.au/view/UQ:27105dd/UQ27105dd_OA.pdf https://espace.library.uq.edu.au/view/UQ:27105dd eng eng Cambridge University Press doi:10.1017/S0007114512000396 issn:0007-1145 issn:1475-2662 orcid:0000-0002-6764-6063 Insulin-like growth factor system Myogenesis Nutrient availability Refeeding and fasting Salmo salar L 2701 Medicine (miscellaneous) 2916 Nutrition and Dietetics Journal Article 2012 ftunivqespace https://doi.org/10.1017/S0007114512000396 2020-08-25T04:32:02Z We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon (Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12°C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle using quantitative real-time PCR at the following postprandial time points: - 12, 0, 1, 3, 6, 12, 24, 48 and 96 h. The gut was fullest 1-6 h after feeding and emptied within 48-96 h. IGF-I, MyoD1c, MRF4 and myf5 transcripts were sharply up-regulated within 1 h of refeeding and are promising candidate genes involved in a fast-response signalling system that regulates fish myotomal muscle growth. These genes clustered together with MyoD1b and suggest a coordinated regulation to favour resumption of myogenesis as an early response to feeding. Insulin-like growth factor (IGF)-II and the ubiquitin ligase MAFbx/atrogin-1 were initially down-regulated but restored to initial values after 12 h. It is also suggested that local production of IGF-I within the muscle might suppress catabolic pathways depressing MAFbx/atrogin-1. Article in Journal/Newspaper Atlantic salmon Salmo salar The University of Queensland: UQ eSpace British Journal of Nutrition 108 12 2148 2157 |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Insulin-like growth factor system Myogenesis Nutrient availability Refeeding and fasting Salmo salar L 2701 Medicine (miscellaneous) 2916 Nutrition and Dietetics |
spellingShingle |
Insulin-like growth factor system Myogenesis Nutrient availability Refeeding and fasting Salmo salar L 2701 Medicine (miscellaneous) 2916 Nutrition and Dietetics Valente, Luísa M.P. Bower, Neil I. Johnston, Ian A. Postprandial expression of growth-related genes in Atlantic salmon (Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation |
topic_facet |
Insulin-like growth factor system Myogenesis Nutrient availability Refeeding and fasting Salmo salar L 2701 Medicine (miscellaneous) 2916 Nutrition and Dietetics |
description |
We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon (Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12°C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle using quantitative real-time PCR at the following postprandial time points: - 12, 0, 1, 3, 6, 12, 24, 48 and 96 h. The gut was fullest 1-6 h after feeding and emptied within 48-96 h. IGF-I, MyoD1c, MRF4 and myf5 transcripts were sharply up-regulated within 1 h of refeeding and are promising candidate genes involved in a fast-response signalling system that regulates fish myotomal muscle growth. These genes clustered together with MyoD1b and suggest a coordinated regulation to favour resumption of myogenesis as an early response to feeding. Insulin-like growth factor (IGF)-II and the ubiquitin ligase MAFbx/atrogin-1 were initially down-regulated but restored to initial values after 12 h. It is also suggested that local production of IGF-I within the muscle might suppress catabolic pathways depressing MAFbx/atrogin-1. |
format |
Article in Journal/Newspaper |
author |
Valente, Luísa M.P. Bower, Neil I. Johnston, Ian A. |
author_facet |
Valente, Luísa M.P. Bower, Neil I. Johnston, Ian A. |
author_sort |
Valente, Luísa M.P. |
title |
Postprandial expression of growth-related genes in Atlantic salmon (Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation |
title_short |
Postprandial expression of growth-related genes in Atlantic salmon (Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation |
title_full |
Postprandial expression of growth-related genes in Atlantic salmon (Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation |
title_fullStr |
Postprandial expression of growth-related genes in Atlantic salmon (Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation |
title_full_unstemmed |
Postprandial expression of growth-related genes in Atlantic salmon (Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation |
title_sort |
postprandial expression of growth-related genes in atlantic salmon (salmo salar l.) juveniles fasted for 1 week and fed a single meal to satiation |
publisher |
Cambridge University Press |
publishDate |
2012 |
url |
https://espace.library.uq.edu.au/view/UQ:27105dd/UQ27105dd_OA.pdf https://espace.library.uq.edu.au/view/UQ:27105dd |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.1017/S0007114512000396 issn:0007-1145 issn:1475-2662 orcid:0000-0002-6764-6063 |
op_doi |
https://doi.org/10.1017/S0007114512000396 |
container_title |
British Journal of Nutrition |
container_volume |
108 |
container_issue |
12 |
container_start_page |
2148 |
op_container_end_page |
2157 |
_version_ |
1766361899650252800 |