Cold adaptation of the Antarctic archaeon, Methanococcoides burtonii assessed by proteomics using ICAT
Using isotope coded affinity tag (ICAT) chromatography and liquid chromatography-mass spectrometry, 163 proteins were identified from the cold-adapted archaeon, Methanococcoides burtonii. 14 proteins were differentially expressed during growth at 4 degrees C and 23 degrees C. Knowledge of protein ab...
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2005
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:112224 2023-05-15T13:31:21+02:00 Cold adaptation of the Antarctic archaeon, Methanococcoides burtonii assessed by proteomics using ICAT Goodchild, A. Raftery, M. Saunders, N. F. W. Guilhaus, M. Cavicchioli, R. 2005-01-01 https://espace.library.uq.edu.au/view/UQ:112224 eng eng Amer Chemical Soc doi:10.1021/pr049760p issn:1535-3893 Biochemical Research Methods Archaea methanogen proteome cold adaptation psychrophile ICAT Coded Affinity Tags Factor 2 Proteins Thermophilic Methanogens Mass-spectrometry Multidimensional Chromatography Hyperthermophilic Archaeon Growth-yield Heat-shock Temperature Psychrotolerant Journal Article 2005 ftunivqespace https://doi.org/10.1021/pr049760p 2020-08-04T06:39:41Z Using isotope coded affinity tag (ICAT) chromatography and liquid chromatography-mass spectrometry, 163 proteins were identified from the cold-adapted archaeon, Methanococcoides burtonii. 14 proteins were differentially expressed during growth at 4 degrees C and 23 degrees C. Knowledge of protein abundance, protein identity and gene arrangement was used to determine mechanisms of cold adaptation. Growth temperature was found to affect proteins involved in energy generation and biosynthesis linked to methanogenesis, membrane transport, transcription and protein folding, as well as affecting the expression of two hypothetical proteins. Pooling the data from this ICAT study with data from a previous two-dimensional gel electrophoresis study highlighted consistencies and differences between the two methods, and led us to conclude that the two approaches were generally complementary. This is the first report of ICAT applied to Archaea, or for the study of cold adaptation in any organism. Article in Journal/Newspaper Antarc* Antarctic The University of Queensland: UQ eSpace Antarctic The Antarctic Journal of Proteome Research 4 2 473 480 |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Biochemical Research Methods Archaea methanogen proteome cold adaptation psychrophile ICAT Coded Affinity Tags Factor 2 Proteins Thermophilic Methanogens Mass-spectrometry Multidimensional Chromatography Hyperthermophilic Archaeon Growth-yield Heat-shock Temperature Psychrotolerant |
spellingShingle |
Biochemical Research Methods Archaea methanogen proteome cold adaptation psychrophile ICAT Coded Affinity Tags Factor 2 Proteins Thermophilic Methanogens Mass-spectrometry Multidimensional Chromatography Hyperthermophilic Archaeon Growth-yield Heat-shock Temperature Psychrotolerant Goodchild, A. Raftery, M. Saunders, N. F. W. Guilhaus, M. Cavicchioli, R. Cold adaptation of the Antarctic archaeon, Methanococcoides burtonii assessed by proteomics using ICAT |
topic_facet |
Biochemical Research Methods Archaea methanogen proteome cold adaptation psychrophile ICAT Coded Affinity Tags Factor 2 Proteins Thermophilic Methanogens Mass-spectrometry Multidimensional Chromatography Hyperthermophilic Archaeon Growth-yield Heat-shock Temperature Psychrotolerant |
description |
Using isotope coded affinity tag (ICAT) chromatography and liquid chromatography-mass spectrometry, 163 proteins were identified from the cold-adapted archaeon, Methanococcoides burtonii. 14 proteins were differentially expressed during growth at 4 degrees C and 23 degrees C. Knowledge of protein abundance, protein identity and gene arrangement was used to determine mechanisms of cold adaptation. Growth temperature was found to affect proteins involved in energy generation and biosynthesis linked to methanogenesis, membrane transport, transcription and protein folding, as well as affecting the expression of two hypothetical proteins. Pooling the data from this ICAT study with data from a previous two-dimensional gel electrophoresis study highlighted consistencies and differences between the two methods, and led us to conclude that the two approaches were generally complementary. This is the first report of ICAT applied to Archaea, or for the study of cold adaptation in any organism. |
format |
Article in Journal/Newspaper |
author |
Goodchild, A. Raftery, M. Saunders, N. F. W. Guilhaus, M. Cavicchioli, R. |
author_facet |
Goodchild, A. Raftery, M. Saunders, N. F. W. Guilhaus, M. Cavicchioli, R. |
author_sort |
Goodchild, A. |
title |
Cold adaptation of the Antarctic archaeon, Methanococcoides burtonii assessed by proteomics using ICAT |
title_short |
Cold adaptation of the Antarctic archaeon, Methanococcoides burtonii assessed by proteomics using ICAT |
title_full |
Cold adaptation of the Antarctic archaeon, Methanococcoides burtonii assessed by proteomics using ICAT |
title_fullStr |
Cold adaptation of the Antarctic archaeon, Methanococcoides burtonii assessed by proteomics using ICAT |
title_full_unstemmed |
Cold adaptation of the Antarctic archaeon, Methanococcoides burtonii assessed by proteomics using ICAT |
title_sort |
cold adaptation of the antarctic archaeon, methanococcoides burtonii assessed by proteomics using icat |
publisher |
Amer Chemical Soc |
publishDate |
2005 |
url |
https://espace.library.uq.edu.au/view/UQ:112224 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
doi:10.1021/pr049760p issn:1535-3893 |
op_doi |
https://doi.org/10.1021/pr049760p |
container_title |
Journal of Proteome Research |
container_volume |
4 |
container_issue |
2 |
container_start_page |
473 |
op_container_end_page |
480 |
_version_ |
1766017655128457216 |