A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii
A global view of the biology of the cold-adapted archaeon Methanococcoides burtonii was achieved using proteomics. Proteins specific to growth at 4degreesC versus T-opt (23degreesC) were identified by mass spectrometry using the draft genome sequence of M. burtonii. mRNA levels were determined for a...
Published in: | Molecular Microbiology |
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Language: | English |
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Blackwell Publishing Ltd
2004
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:111602 2023-05-15T13:31:21+02:00 A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii Goodchild, A. Saunders, N. F. W. Ertan, H. Raftery, M. Guilhaus, M Curmi, P. M. G Cavicchioli, R. 2004-01-01 https://espace.library.uq.edu.au/view/UQ:111602 eng eng Blackwell Publishing Ltd doi:10.1111/j.1365-2958.2004.04130.x issn:0950-382X Biochemistry & Molecular Biology Microbiology Methanosarcina-barkeri Rna-polymerase Hyperthermophilic Archaeum Methanogenium-frigidum Ace Lake Protein Methyltransferase Transcription Pyrrolysine Stress 0605 Microbiology 1108 Medical Microbiology Journal Article 2004 ftunivqespace https://doi.org/10.1111/j.1365-2958.2004.04130.x 2020-08-04T06:41:48Z A global view of the biology of the cold-adapted archaeon Methanococcoides burtonii was achieved using proteomics. Proteins specific to growth at 4degreesC versus T-opt (23degreesC) were identified by mass spectrometry using the draft genome sequence of M. burtonii. mRNA levels were determined for all genes identified by proteomics, and specific enzyme assays confirmed the protein expression results. Key aspects of cold adaptation related to transcription, protein folding and metabolism, including specific roles for RNA polymerase subunit E, a response regulator and peptidyl prolyl cis/trans isomerase. Heat shock protein DnaK was expressed during growth at T-opt, indicating that growth at 'optimal' temperatures was stressful for this cold-adapted organism. Expression of trimethylamine methyltransferase involves contiguous translation of two open reading frames, which is likely to result from incorporation of pyrrolysine at an amber stop codon. Thermal regulation in M. burtonii is achieved through complex gene expression events involving gene clusters and operons, through to protein modifications. Article in Journal/Newspaper Antarc* Antarctic The University of Queensland: UQ eSpace Antarctic The Antarctic Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Molecular Microbiology 53 1 309 321 |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Biochemistry & Molecular Biology Microbiology Methanosarcina-barkeri Rna-polymerase Hyperthermophilic Archaeum Methanogenium-frigidum Ace Lake Protein Methyltransferase Transcription Pyrrolysine Stress 0605 Microbiology 1108 Medical Microbiology |
spellingShingle |
Biochemistry & Molecular Biology Microbiology Methanosarcina-barkeri Rna-polymerase Hyperthermophilic Archaeum Methanogenium-frigidum Ace Lake Protein Methyltransferase Transcription Pyrrolysine Stress 0605 Microbiology 1108 Medical Microbiology Goodchild, A. Saunders, N. F. W. Ertan, H. Raftery, M. Guilhaus, M Curmi, P. M. G Cavicchioli, R. A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii |
topic_facet |
Biochemistry & Molecular Biology Microbiology Methanosarcina-barkeri Rna-polymerase Hyperthermophilic Archaeum Methanogenium-frigidum Ace Lake Protein Methyltransferase Transcription Pyrrolysine Stress 0605 Microbiology 1108 Medical Microbiology |
description |
A global view of the biology of the cold-adapted archaeon Methanococcoides burtonii was achieved using proteomics. Proteins specific to growth at 4degreesC versus T-opt (23degreesC) were identified by mass spectrometry using the draft genome sequence of M. burtonii. mRNA levels were determined for all genes identified by proteomics, and specific enzyme assays confirmed the protein expression results. Key aspects of cold adaptation related to transcription, protein folding and metabolism, including specific roles for RNA polymerase subunit E, a response regulator and peptidyl prolyl cis/trans isomerase. Heat shock protein DnaK was expressed during growth at T-opt, indicating that growth at 'optimal' temperatures was stressful for this cold-adapted organism. Expression of trimethylamine methyltransferase involves contiguous translation of two open reading frames, which is likely to result from incorporation of pyrrolysine at an amber stop codon. Thermal regulation in M. burtonii is achieved through complex gene expression events involving gene clusters and operons, through to protein modifications. |
format |
Article in Journal/Newspaper |
author |
Goodchild, A. Saunders, N. F. W. Ertan, H. Raftery, M. Guilhaus, M Curmi, P. M. G Cavicchioli, R. |
author_facet |
Goodchild, A. Saunders, N. F. W. Ertan, H. Raftery, M. Guilhaus, M Curmi, P. M. G Cavicchioli, R. |
author_sort |
Goodchild, A. |
title |
A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii |
title_short |
A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii |
title_full |
A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii |
title_fullStr |
A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii |
title_full_unstemmed |
A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii |
title_sort |
proteomic determination of cold adaptation in the antarctic archaeon, methanococcoides burtonii |
publisher |
Blackwell Publishing Ltd |
publishDate |
2004 |
url |
https://espace.library.uq.edu.au/view/UQ:111602 |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) |
geographic |
Antarctic The Antarctic Ace Lake |
geographic_facet |
Antarctic The Antarctic Ace Lake |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
doi:10.1111/j.1365-2958.2004.04130.x issn:0950-382X |
op_doi |
https://doi.org/10.1111/j.1365-2958.2004.04130.x |
container_title |
Molecular Microbiology |
container_volume |
53 |
container_issue |
1 |
container_start_page |
309 |
op_container_end_page |
321 |
_version_ |
1766017656970805248 |