Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP)
The ELISPOT assay was used to measure the number of specific antibody secreting cells (ASC) induced during the primary and secondary immune responses in the spleen, head kidney and gut of juvenile (5 g) sea bass (Dicentrarchus labrax) to bacterial (Vibrio anguillarum and Photobacterium damselae ssp....
Published in: | Fish & Shellfish Immunology |
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Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Academic Press Ltd
2001
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Online Access: | https://espace.library.uq.edu.au/view/UQ:109379 |
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:109379 2023-05-15T18:10:01+02:00 Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP) Dos Santos, Nuno M. S. Taverne-Thiele, J. J. Barnes, Andrew C. Ellis, Anthony E. Rombout, Jan H. W. M. 2001-05-01 https://espace.library.uq.edu.au/view/UQ:109379 eng eng Academic Press Ltd doi:10.1006/fsim.2000.0320 issn:1050-4648 orcid:0000-0002-3990-8070 Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences fish sea bass Dicentrarchus labrax ELISPOT antibody-secreting cells kinetics Photobacterium damselae ssp piscicida Vibrio anguillarum DNP-KLH Trout Oncorhynchus-mykiss Salmo-salar L Carp Cyprinus-carpio Rainbow-trout Elispot-assay Aeromonas-salmonicida Immune-response Induced Suppression Yersinia-ruckeri Journal Article 2001 ftunivqespace https://doi.org/10.1006/fsim.2000.0320 2020-08-04T06:35:27Z The ELISPOT assay was used to measure the number of specific antibody secreting cells (ASC) induced during the primary and secondary immune responses in the spleen, head kidney and gut of juvenile (5 g) sea bass (Dicentrarchus labrax) to bacterial (Vibrio anguillarum and Photobacterium damselae ssp. piscicida) and hapten dinitrophenyl-conjugated to keyhole limpet haemocyanin (DNP-KLH) antigens administered intraperitoneally. High variability among individuals was observed at each sampling day. All fish were bath vaccinated to V. anguillarum at an earlier stage (2 g) in the farm of origin prior to the development of the experiments, and therefore only secondary and tertiary responses were measured in the group immunised with this bacterium. Significant differences to the controls were observed in the primary responses of the head kidney and the spleen to P. damselae ssp. piscicida and DNP, respectively. Frequency analysis of the production of ASC suggests that significant responses in the gut might be masked by the high error variance. The peak of the primary response was observed 4 days earlier to DNP (18-20 days post-immunisation) and it was significantly higher than the response to P. damselae ssp. piscicida. Higher numbers of ASC were observed in the secondary responses of the head kidney and spleen, although they were not statistically significantly different from the primary levels, probably due to the high error variance as supported by the frequency analysis. Nevertheless, together with a faster response (peak at 7 days post-immunisation), the data suggest that memory formation had occurred. Additionally, the data suggest that some suppression of the secondary immune response in the gut might have occurred. The head kidney appears to produce the highest number of specific ASC of the organs tested. It appears that sea bass show a relatively fast but short duration antibody response. (C) 2001 Academic Press. Article in Journal/Newspaper Salmo salar The University of Queensland: UQ eSpace Keyhole ENVELOPE(-67.338,-67.338,-68.785,-68.785) Fish & Shellfish Immunology 11 4 317 331 |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences fish sea bass Dicentrarchus labrax ELISPOT antibody-secreting cells kinetics Photobacterium damselae ssp piscicida Vibrio anguillarum DNP-KLH Trout Oncorhynchus-mykiss Salmo-salar L Carp Cyprinus-carpio Rainbow-trout Elispot-assay Aeromonas-salmonicida Immune-response Induced Suppression Yersinia-ruckeri |
spellingShingle |
Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences fish sea bass Dicentrarchus labrax ELISPOT antibody-secreting cells kinetics Photobacterium damselae ssp piscicida Vibrio anguillarum DNP-KLH Trout Oncorhynchus-mykiss Salmo-salar L Carp Cyprinus-carpio Rainbow-trout Elispot-assay Aeromonas-salmonicida Immune-response Induced Suppression Yersinia-ruckeri Dos Santos, Nuno M. S. Taverne-Thiele, J. J. Barnes, Andrew C. Ellis, Anthony E. Rombout, Jan H. W. M. Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP) |
topic_facet |
Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences fish sea bass Dicentrarchus labrax ELISPOT antibody-secreting cells kinetics Photobacterium damselae ssp piscicida Vibrio anguillarum DNP-KLH Trout Oncorhynchus-mykiss Salmo-salar L Carp Cyprinus-carpio Rainbow-trout Elispot-assay Aeromonas-salmonicida Immune-response Induced Suppression Yersinia-ruckeri |
description |
The ELISPOT assay was used to measure the number of specific antibody secreting cells (ASC) induced during the primary and secondary immune responses in the spleen, head kidney and gut of juvenile (5 g) sea bass (Dicentrarchus labrax) to bacterial (Vibrio anguillarum and Photobacterium damselae ssp. piscicida) and hapten dinitrophenyl-conjugated to keyhole limpet haemocyanin (DNP-KLH) antigens administered intraperitoneally. High variability among individuals was observed at each sampling day. All fish were bath vaccinated to V. anguillarum at an earlier stage (2 g) in the farm of origin prior to the development of the experiments, and therefore only secondary and tertiary responses were measured in the group immunised with this bacterium. Significant differences to the controls were observed in the primary responses of the head kidney and the spleen to P. damselae ssp. piscicida and DNP, respectively. Frequency analysis of the production of ASC suggests that significant responses in the gut might be masked by the high error variance. The peak of the primary response was observed 4 days earlier to DNP (18-20 days post-immunisation) and it was significantly higher than the response to P. damselae ssp. piscicida. Higher numbers of ASC were observed in the secondary responses of the head kidney and spleen, although they were not statistically significantly different from the primary levels, probably due to the high error variance as supported by the frequency analysis. Nevertheless, together with a faster response (peak at 7 days post-immunisation), the data suggest that memory formation had occurred. Additionally, the data suggest that some suppression of the secondary immune response in the gut might have occurred. The head kidney appears to produce the highest number of specific ASC of the organs tested. It appears that sea bass show a relatively fast but short duration antibody response. (C) 2001 Academic Press. |
format |
Article in Journal/Newspaper |
author |
Dos Santos, Nuno M. S. Taverne-Thiele, J. J. Barnes, Andrew C. Ellis, Anthony E. Rombout, Jan H. W. M. |
author_facet |
Dos Santos, Nuno M. S. Taverne-Thiele, J. J. Barnes, Andrew C. Ellis, Anthony E. Rombout, Jan H. W. M. |
author_sort |
Dos Santos, Nuno M. S. |
title |
Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP) |
title_short |
Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP) |
title_full |
Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP) |
title_fullStr |
Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP) |
title_full_unstemmed |
Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP) |
title_sort |
kinetics of juvenile sea bass (dicentrarchus labrax, l.) systemic and mucosal antibody secreting cell response to different antigens (photobacterium damselae spp. piscicida, vibrio anguillarum and dnp) |
publisher |
Academic Press Ltd |
publishDate |
2001 |
url |
https://espace.library.uq.edu.au/view/UQ:109379 |
long_lat |
ENVELOPE(-67.338,-67.338,-68.785,-68.785) |
geographic |
Keyhole |
geographic_facet |
Keyhole |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
doi:10.1006/fsim.2000.0320 issn:1050-4648 orcid:0000-0002-3990-8070 |
op_doi |
https://doi.org/10.1006/fsim.2000.0320 |
container_title |
Fish & Shellfish Immunology |
container_volume |
11 |
container_issue |
4 |
container_start_page |
317 |
op_container_end_page |
331 |
_version_ |
1766182739664437248 |