Cloning, sequences, and characterization of two chitinase genes from the antarctic Arthrobacter sp strain TAD20: Isolation and partial characterization of the enzymes
Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA and ChiB (ArChiA and ArChiB), in response to chitin induction. A single chromosomal DNA fragment con...
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American Society for Microbiology
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:109195 2023-05-15T13:55:31+02:00 Cloning, sequences, and characterization of two chitinase genes from the antarctic Arthrobacter sp strain TAD20: Isolation and partial characterization of the enzymes Lonhienne, Thierry Mavromatis, Konstantinos Vorgias, Constantin E. Buchon, Laurent Gerday, Charles Bouriotis, Vassilis 2001-03-01 https://espace.library.uq.edu.au/view/UQ:109195/UQ109195_OA.pdf https://espace.library.uq.edu.au/view/UQ:109195 eng eng American Society for Microbiology doi:10.1128/JB.183.5.1773-1779.2001 issn:0021-9193 Microbiology Bacterium Vibrio-furnissii Bacillus-circulans Wl-12 Marine Bacterium Binding Domain Alteromonas-haloplanctis Serratia-marcescens Glycosyl Hydrolases Molecular-cloning Catabolic Cascade Crystal-structure Journal Article 2001 ftunivqespace https://doi.org/10.1128/JB.183.5.1773-1779.2001 2020-10-26T23:48:13Z Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA and ChiB (ArChiA and ArChiB), in response to chitin induction. A single chromosomal DNA fragment containing the genes coding for both chitinases was cloned in Escherichia coli. DNA sequencing analysis of this fragment revealed two contiguous open reading frames coding for the precursors of ArChiA (881 amino acids [aa]) and ArChiB (578 aa). ArChiA and ArChiB are modular enzymes consisting of a glycosyl-hydrolase family 18 catalytic domain as well as two and one chitin-binding domains, respectively. The catalytic domain of ArChiA exhibits 55% identity with a chitodextrinase from Vibro furnissii. The ArChiB catalytic domain exhibits 33% identity with chitinase A of Bacillus circulans. The ArChiA chitin-binding domains are homologous to the chitin-binding domain of ArChiB. ArChiA and ArChiB were purified to homogeneity from the native Arthrobacter strain and partially characterized. Thermal unfolding of ArChiA, ArChiB, and chitinase A of Serratia marcescens was studied using differential scanning calorimetry. ArChiA and ArChiB, compared to their mesophilic counterpart, exhibited increased heat lability, similar to other cold-adapted enzymes. Article in Journal/Newspaper Antarc* Antarctic The University of Queensland: UQ eSpace Antarctic The Antarctic Journal of Bacteriology 183 5 1773 1779 |
institution |
Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Microbiology Bacterium Vibrio-furnissii Bacillus-circulans Wl-12 Marine Bacterium Binding Domain Alteromonas-haloplanctis Serratia-marcescens Glycosyl Hydrolases Molecular-cloning Catabolic Cascade Crystal-structure |
spellingShingle |
Microbiology Bacterium Vibrio-furnissii Bacillus-circulans Wl-12 Marine Bacterium Binding Domain Alteromonas-haloplanctis Serratia-marcescens Glycosyl Hydrolases Molecular-cloning Catabolic Cascade Crystal-structure Lonhienne, Thierry Mavromatis, Konstantinos Vorgias, Constantin E. Buchon, Laurent Gerday, Charles Bouriotis, Vassilis Cloning, sequences, and characterization of two chitinase genes from the antarctic Arthrobacter sp strain TAD20: Isolation and partial characterization of the enzymes |
topic_facet |
Microbiology Bacterium Vibrio-furnissii Bacillus-circulans Wl-12 Marine Bacterium Binding Domain Alteromonas-haloplanctis Serratia-marcescens Glycosyl Hydrolases Molecular-cloning Catabolic Cascade Crystal-structure |
description |
Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA and ChiB (ArChiA and ArChiB), in response to chitin induction. A single chromosomal DNA fragment containing the genes coding for both chitinases was cloned in Escherichia coli. DNA sequencing analysis of this fragment revealed two contiguous open reading frames coding for the precursors of ArChiA (881 amino acids [aa]) and ArChiB (578 aa). ArChiA and ArChiB are modular enzymes consisting of a glycosyl-hydrolase family 18 catalytic domain as well as two and one chitin-binding domains, respectively. The catalytic domain of ArChiA exhibits 55% identity with a chitodextrinase from Vibro furnissii. The ArChiB catalytic domain exhibits 33% identity with chitinase A of Bacillus circulans. The ArChiA chitin-binding domains are homologous to the chitin-binding domain of ArChiB. ArChiA and ArChiB were purified to homogeneity from the native Arthrobacter strain and partially characterized. Thermal unfolding of ArChiA, ArChiB, and chitinase A of Serratia marcescens was studied using differential scanning calorimetry. ArChiA and ArChiB, compared to their mesophilic counterpart, exhibited increased heat lability, similar to other cold-adapted enzymes. |
format |
Article in Journal/Newspaper |
author |
Lonhienne, Thierry Mavromatis, Konstantinos Vorgias, Constantin E. Buchon, Laurent Gerday, Charles Bouriotis, Vassilis |
author_facet |
Lonhienne, Thierry Mavromatis, Konstantinos Vorgias, Constantin E. Buchon, Laurent Gerday, Charles Bouriotis, Vassilis |
author_sort |
Lonhienne, Thierry |
title |
Cloning, sequences, and characterization of two chitinase genes from the antarctic Arthrobacter sp strain TAD20: Isolation and partial characterization of the enzymes |
title_short |
Cloning, sequences, and characterization of two chitinase genes from the antarctic Arthrobacter sp strain TAD20: Isolation and partial characterization of the enzymes |
title_full |
Cloning, sequences, and characterization of two chitinase genes from the antarctic Arthrobacter sp strain TAD20: Isolation and partial characterization of the enzymes |
title_fullStr |
Cloning, sequences, and characterization of two chitinase genes from the antarctic Arthrobacter sp strain TAD20: Isolation and partial characterization of the enzymes |
title_full_unstemmed |
Cloning, sequences, and characterization of two chitinase genes from the antarctic Arthrobacter sp strain TAD20: Isolation and partial characterization of the enzymes |
title_sort |
cloning, sequences, and characterization of two chitinase genes from the antarctic arthrobacter sp strain tad20: isolation and partial characterization of the enzymes |
publisher |
American Society for Microbiology |
publishDate |
2001 |
url |
https://espace.library.uq.edu.au/view/UQ:109195/UQ109195_OA.pdf https://espace.library.uq.edu.au/view/UQ:109195 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
doi:10.1128/JB.183.5.1773-1779.2001 issn:0021-9193 |
op_doi |
https://doi.org/10.1128/JB.183.5.1773-1779.2001 |
container_title |
Journal of Bacteriology |
container_volume |
183 |
container_issue |
5 |
container_start_page |
1773 |
op_container_end_page |
1779 |
_version_ |
1766262183044317184 |