Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD)
Amoebic gill disease (AGD) is one of the main health issues impacting farmed Atlantic salmon. Neoparamoeba perurans causes AGD; however, a diversity of other amoeba species colonizes the gills and there is little understanding of whether they are commensal or potentially involved in different stages...
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2020
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ftunivqespace:oai:espace.library.uq.edu.au:UQ:0f084b6 2023-05-15T15:30:08+02:00 Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) English, Chloe J. Botwright, Natasha A. Adams, Mark B. Barnes, Andrew C. Wynne, James W. Lima, Paula C. Cook, Mathew T. 2020-12-12 https://espace.library.uq.edu.au/view/UQ:0f084b6 eng eng Wiley-Blackwell doi:10.1111/jfd.13319 issn:1365-2761 issn:0140-7775 orcid:0000-0002-9904-493X orcid:0000-0002-3990-8070 Not set Amoebozoa aetiology aquaculture gill disease histopathology 1104 Aquatic Science 3401 Veterinary (miscalleneous) Journal Article 2020 ftunivqespace https://doi.org/10.1111/jfd.13319 2020-12-29T01:42:50Z Amoebic gill disease (AGD) is one of the main health issues impacting farmed Atlantic salmon. Neoparamoeba perurans causes AGD; however, a diversity of other amoeba species colonizes the gills and there is little understanding of whether they are commensal or potentially involved in different stages of gill disease development. Here, we conduct in vivo challenges of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. to investigate their pathogenicity to Atlantic salmon gills. Additionally, we assessed whether the presence of Nolandella sp. and Pseudoparamoeba sp. influences the onset and/or severity of N. perurans-induced AGD. All three strains attached and multiplied on the gills according to qPCR analysis. Furthermore, minor gross gill lesions and histological changes were observed post-exposure. While N. perurans was found associated with classical AGD lesions, Nolandella sp. and Pseudoparamoeba sp. were not found associated with lesion sites and these lesions did not meet the expected composite of histopathological changes for AGD. Moreover, the presence of these non-N. perurans species did not significantly increase the severity of AGD. This trial provides evidence that cultured Nolandella sp. and Pseudoparamoeba sp. do not induce AGD and do not influence the severity of AGD during the early stages of development. Article in Journal/Newspaper Atlantic salmon The University of Queensland: UQ eSpace Journal of Fish Diseases 44 2 149 160 |
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Open Polar |
collection |
The University of Queensland: UQ eSpace |
op_collection_id |
ftunivqespace |
language |
English |
topic |
Amoebozoa aetiology aquaculture gill disease histopathology 1104 Aquatic Science 3401 Veterinary (miscalleneous) |
spellingShingle |
Amoebozoa aetiology aquaculture gill disease histopathology 1104 Aquatic Science 3401 Veterinary (miscalleneous) English, Chloe J. Botwright, Natasha A. Adams, Mark B. Barnes, Andrew C. Wynne, James W. Lima, Paula C. Cook, Mathew T. Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) |
topic_facet |
Amoebozoa aetiology aquaculture gill disease histopathology 1104 Aquatic Science 3401 Veterinary (miscalleneous) |
description |
Amoebic gill disease (AGD) is one of the main health issues impacting farmed Atlantic salmon. Neoparamoeba perurans causes AGD; however, a diversity of other amoeba species colonizes the gills and there is little understanding of whether they are commensal or potentially involved in different stages of gill disease development. Here, we conduct in vivo challenges of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. to investigate their pathogenicity to Atlantic salmon gills. Additionally, we assessed whether the presence of Nolandella sp. and Pseudoparamoeba sp. influences the onset and/or severity of N. perurans-induced AGD. All three strains attached and multiplied on the gills according to qPCR analysis. Furthermore, minor gross gill lesions and histological changes were observed post-exposure. While N. perurans was found associated with classical AGD lesions, Nolandella sp. and Pseudoparamoeba sp. were not found associated with lesion sites and these lesions did not meet the expected composite of histopathological changes for AGD. Moreover, the presence of these non-N. perurans species did not significantly increase the severity of AGD. This trial provides evidence that cultured Nolandella sp. and Pseudoparamoeba sp. do not induce AGD and do not influence the severity of AGD during the early stages of development. |
format |
Article in Journal/Newspaper |
author |
English, Chloe J. Botwright, Natasha A. Adams, Mark B. Barnes, Andrew C. Wynne, James W. Lima, Paula C. Cook, Mathew T. |
author_facet |
English, Chloe J. Botwright, Natasha A. Adams, Mark B. Barnes, Andrew C. Wynne, James W. Lima, Paula C. Cook, Mathew T. |
author_sort |
English, Chloe J. |
title |
Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) |
title_short |
Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) |
title_full |
Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) |
title_fullStr |
Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) |
title_full_unstemmed |
Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans-induced amoebic gill disease (AGD) |
title_sort |
immersion challenge of naïve atlantic salmon with cultured nolandella sp. and pseudoparamoeba sp. did not increase the severity of neoparamoeba perurans-induced amoebic gill disease (agd) |
publisher |
Wiley-Blackwell |
publishDate |
2020 |
url |
https://espace.library.uq.edu.au/view/UQ:0f084b6 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
doi:10.1111/jfd.13319 issn:1365-2761 issn:0140-7775 orcid:0000-0002-9904-493X orcid:0000-0002-3990-8070 Not set |
op_doi |
https://doi.org/10.1111/jfd.13319 |
container_title |
Journal of Fish Diseases |
container_volume |
44 |
container_issue |
2 |
container_start_page |
149 |
op_container_end_page |
160 |
_version_ |
1766360581626920960 |