Role of calcium on the initiation of sperm motility in the European eel
[EN] Sperm from European eel males treated with hCG(rec), was washed in a calcium free extender, and sperm motility was activated both in the presence (seawater, SW) and in the absence of calcium (NaCI + EDTA), and treated with calcium inhibitors or modulators. The sperm motility parameters were eva...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
---|---|
Main Authors: | , , , , , |
Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2016
|
Subjects: | |
Online Access: | http://hdl.handle.net/10251/79931 https://doi.org/10.1016/j.cbpa.2015.10.009 |
id |
ftunivpvalencia:oai:riunet.upv.es:10251/79931 |
---|---|
record_format |
openpolar |
spelling |
ftunivpvalencia:oai:riunet.upv.es:10251/79931 2023-05-15T13:28:19+02:00 Role of calcium on the initiation of sperm motility in the European eel Pérez Igualada, Luz María Vilchez Olivencia, Maria Carmen Gallego Albiach, Victor MORINI, MARINA Peñaranda, D.S. Asturiano Nemesio, Juan Francisco Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural Ministerio de Ciencia e Innovación 2016-01 http://hdl.handle.net/10251/79931 https://doi.org/10.1016/j.cbpa.2015.10.009 eng eng Elsevier Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology info:eu-repo/grantAgreement/MICINN//AGL2010-16009/ES/MECANISMOS FISIOLOGICOS IMPLICADOS EN LA ESPERMIACION Y EN LA ADQUISICION DE MOTILIDAD ESPERMATICA EN LA ANGUILA EUROPEA (ANGUILLA ANGUILLA)/ http://dx.doi.org/ 10.1016/j.cbpa.2015.10.009 urn:issn:1095-6433 http://hdl.handle.net/10251/79931 doi:10.1016/j.cbpa.2015.10.009 urn:eissn:1531-4332 26459984 http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess CC-BY-NC-ND Anguilla anguilla Calcium/sodium exchanger Flow cytometry Intracellular ions Sperm activation Sperm physiology Sperm kinetics BIOLOGIA ANIMAL PRODUCCION ANIMAL info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2016 ftunivpvalencia https://doi.org/10.1016/j.cbpa.2015.10.009 2022-06-12T20:41:49Z [EN] Sperm from European eel males treated with hCG(rec), was washed in a calcium free extender, and sperm motility was activated both in the presence (seawater, SW) and in the absence of calcium (NaCI + EDTA), and treated with calcium inhibitors or modulators. The sperm motility parameters were evaluated by a computer-assisted sperm analysis (CASA) system, and changes in the [Ca2+](i) fluorescence (and in [Na+](i) in some cases) were evaluated by flow cytometry. After sperm motility was activated in a medium containing Ca2+ (seawater, SW) the intracellular fluorescence emitted by Ca2+ increased 4-6-fold compared to the levels in quiescent sperm. However, while sperm activation in a Ca-free media (NaCI + EDTA) resulted in a percentage of motility similar to seawater, the [Ca2+](i) levels did not increase at all. This result strongly suggests that increasing [Ca2+](i) is not a pre-requisite for the induction of sperm motility in European eel sperm. Several sperm velocities (VCL, VSL, VAP) decreased when sperm was activated in the Ca-free activator, thus supporting the theory that Ca2+ has a modulatory effect on sperm motility. The results indicate that a calcium/sodium exchanger (NCX) which is inhibited by bepridil and a calcium calmodulin kinase (inhibited by W-7), are involved in the sperm motility of the European eel. Our results indicate that the increase in [Ca2+](i) concentrations during sperm activation is due to an influx from the external medium, but, unlike in most other species, it does not appear to be necessary for the activation of motility in European eel sperm. (c) 2015 Elsevier Inc All rights reserved. Funded from the SPERMOT project (Spanish Ministry of Science and Innovation, MICINN; AGL2010-16009). M.C. Vilchez has a predoctoral grant from UPV PAID Program (2011-S2-02-6521), Marina Morini has a predoctoral grant from Generalitat Valenciana (Programa Grisolia, GRISOLIA/2012/006), Victor Gallego has a postdoctoral contract from UPV (PAID-10-14), and David S. Penaranda was supported by MICINN ... Article in Journal/Newspaper Anguilla anguilla European eel Politechnical University of Valencia: RiuNet Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 191 98 106 |
institution |
Open Polar |
collection |
Politechnical University of Valencia: RiuNet |
op_collection_id |
ftunivpvalencia |
language |
English |
topic |
Anguilla anguilla Calcium/sodium exchanger Flow cytometry Intracellular ions Sperm activation Sperm physiology Sperm kinetics BIOLOGIA ANIMAL PRODUCCION ANIMAL |
spellingShingle |
Anguilla anguilla Calcium/sodium exchanger Flow cytometry Intracellular ions Sperm activation Sperm physiology Sperm kinetics BIOLOGIA ANIMAL PRODUCCION ANIMAL Pérez Igualada, Luz María Vilchez Olivencia, Maria Carmen Gallego Albiach, Victor MORINI, MARINA Peñaranda, D.S. Asturiano Nemesio, Juan Francisco Role of calcium on the initiation of sperm motility in the European eel |
topic_facet |
Anguilla anguilla Calcium/sodium exchanger Flow cytometry Intracellular ions Sperm activation Sperm physiology Sperm kinetics BIOLOGIA ANIMAL PRODUCCION ANIMAL |
description |
[EN] Sperm from European eel males treated with hCG(rec), was washed in a calcium free extender, and sperm motility was activated both in the presence (seawater, SW) and in the absence of calcium (NaCI + EDTA), and treated with calcium inhibitors or modulators. The sperm motility parameters were evaluated by a computer-assisted sperm analysis (CASA) system, and changes in the [Ca2+](i) fluorescence (and in [Na+](i) in some cases) were evaluated by flow cytometry. After sperm motility was activated in a medium containing Ca2+ (seawater, SW) the intracellular fluorescence emitted by Ca2+ increased 4-6-fold compared to the levels in quiescent sperm. However, while sperm activation in a Ca-free media (NaCI + EDTA) resulted in a percentage of motility similar to seawater, the [Ca2+](i) levels did not increase at all. This result strongly suggests that increasing [Ca2+](i) is not a pre-requisite for the induction of sperm motility in European eel sperm. Several sperm velocities (VCL, VSL, VAP) decreased when sperm was activated in the Ca-free activator, thus supporting the theory that Ca2+ has a modulatory effect on sperm motility. The results indicate that a calcium/sodium exchanger (NCX) which is inhibited by bepridil and a calcium calmodulin kinase (inhibited by W-7), are involved in the sperm motility of the European eel. Our results indicate that the increase in [Ca2+](i) concentrations during sperm activation is due to an influx from the external medium, but, unlike in most other species, it does not appear to be necessary for the activation of motility in European eel sperm. (c) 2015 Elsevier Inc All rights reserved. Funded from the SPERMOT project (Spanish Ministry of Science and Innovation, MICINN; AGL2010-16009). M.C. Vilchez has a predoctoral grant from UPV PAID Program (2011-S2-02-6521), Marina Morini has a predoctoral grant from Generalitat Valenciana (Programa Grisolia, GRISOLIA/2012/006), Victor Gallego has a postdoctoral contract from UPV (PAID-10-14), and David S. Penaranda was supported by MICINN ... |
author2 |
Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural Ministerio de Ciencia e Innovación |
format |
Article in Journal/Newspaper |
author |
Pérez Igualada, Luz María Vilchez Olivencia, Maria Carmen Gallego Albiach, Victor MORINI, MARINA Peñaranda, D.S. Asturiano Nemesio, Juan Francisco |
author_facet |
Pérez Igualada, Luz María Vilchez Olivencia, Maria Carmen Gallego Albiach, Victor MORINI, MARINA Peñaranda, D.S. Asturiano Nemesio, Juan Francisco |
author_sort |
Pérez Igualada, Luz María |
title |
Role of calcium on the initiation of sperm motility in the European eel |
title_short |
Role of calcium on the initiation of sperm motility in the European eel |
title_full |
Role of calcium on the initiation of sperm motility in the European eel |
title_fullStr |
Role of calcium on the initiation of sperm motility in the European eel |
title_full_unstemmed |
Role of calcium on the initiation of sperm motility in the European eel |
title_sort |
role of calcium on the initiation of sperm motility in the european eel |
publisher |
Elsevier |
publishDate |
2016 |
url |
http://hdl.handle.net/10251/79931 https://doi.org/10.1016/j.cbpa.2015.10.009 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology info:eu-repo/grantAgreement/MICINN//AGL2010-16009/ES/MECANISMOS FISIOLOGICOS IMPLICADOS EN LA ESPERMIACION Y EN LA ADQUISICION DE MOTILIDAD ESPERMATICA EN LA ANGUILA EUROPEA (ANGUILLA ANGUILLA)/ http://dx.doi.org/ 10.1016/j.cbpa.2015.10.009 urn:issn:1095-6433 http://hdl.handle.net/10251/79931 doi:10.1016/j.cbpa.2015.10.009 urn:eissn:1531-4332 26459984 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.cbpa.2015.10.009 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
191 |
container_start_page |
98 |
op_container_end_page |
106 |
_version_ |
1766403386067910656 |