Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene
[EN] Recent studies have demonstrated the impact of diet on microbiota composition, but the essential need for the optimization of production rates and costs forces farms and aquaculture production to carry out continuous dietary tests. In order to understand the effect of total fishmeal replacement...
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Online Access: | http://hdl.handle.net/10251/65883 https://doi.org/10.1371/journal.pone.0136389 |
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ftunivpvalencia:oai:riunet.upv.es:10251/65883 2023-05-15T15:33:01+02:00 Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene Estruch, Guillem Collado Amores, María Carmen Peñaranda, D.S. Tomas-Vidal, A. Jover Cerdá, Miguel Pérez-Martínez, G. Martínez-Llorens, Silvia Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal Universitat Politècnica de València 2015-08 http://hdl.handle.net/10251/65883 https://doi.org/10.1371/journal.pone.0136389 eng eng Public Library of Science PLoS ONE info:eu-repo/grantAgreement/UPV//SP20120603/ https://dx.doi.org/10.1371/journal.pone.0136389 urn:issn:1932-6203 http://hdl.handle.net/10251/65883 doi:10.1371/journal.pone.0136389 26317431 PMC4552794 http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess CC-BY SALMO-SALAR L TROUT ONCORHYNCHUS-MYKISS FARMED ATLANTIC SALMON STANDARD SOYBEAN-MEAL PLANT PROTEIN-SOURCES INTESTINAL MICROBIOTA GUT MICROBIOTA BACTERIAL COMMUNITIES NUTRIENT UTILIZATION AQUACULTURE SYSTEMS BIOLOGIA ANIMAL PRODUCCION ANIMAL info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2015 ftunivpvalencia https://doi.org/10.1371/journal.pone.0136389 2022-06-12T20:39:16Z [EN] Recent studies have demonstrated the impact of diet on microbiota composition, but the essential need for the optimization of production rates and costs forces farms and aquaculture production to carry out continuous dietary tests. In order to understand the effect of total fishmeal replacement by vegetable-based feed in the sea bream (Sparus aurata), the microbial composition of the stomach, foregut, midgut and hindgut was analysed using high-throughput 16S rDNA sequencing, also considering parameters of growth, survival and nutrient utilisation indices.A total of 91,539 16S rRNA filtered-sequences were analysed, with an average number of 3661.56 taxonomically assigned, high-quality sequences per sample. The dominant phyla throughout the whole gastrointestinal tract were Actinobacteria, Protebacteria and Firmicutes. A lower diversity in the stomach in comparison to the other intestinal sections was observed. The microbial composition of the Recirculating Aquaculture System was totally different to that of the sea bream gastrointestinal tract. Total fishmeal replacement had an important impact on microbial profiles but not on diversity. Streptococcus (p-value: 0.043) and Photobacterium (p-value: 0.025) were highly represented in fish fed with fishmeal and vegetable-meal diets, respectively. In the stomach samples with the vegetable diet, reads of chloroplasts and mitochondria from vegetable dietary ingredients were rather abundant. Principal Coordinate Analysis showed a clear differentiation between diets in the microbiota present in the gut, supporting the presence of specific bacterial consortia associated with the diet.Although differences in growth and nutritive parameters were not observed, a negative effect of the vegetable diet on the survival rate was determined. Further studies are required to shed more light on the relationship between the immune system and sea bream gastrointestinal tract microbiota and should consider the modulation of the microbiota to improve the survival rate and nutritive ... Article in Journal/Newspaper Atlantic salmon Salmo salar Politechnical University of Valencia: RiuNet PLOS ONE 10 8 e0136389 |
institution |
Open Polar |
collection |
Politechnical University of Valencia: RiuNet |
op_collection_id |
ftunivpvalencia |
language |
English |
topic |
SALMO-SALAR L TROUT ONCORHYNCHUS-MYKISS FARMED ATLANTIC SALMON STANDARD SOYBEAN-MEAL PLANT PROTEIN-SOURCES INTESTINAL MICROBIOTA GUT MICROBIOTA BACTERIAL COMMUNITIES NUTRIENT UTILIZATION AQUACULTURE SYSTEMS BIOLOGIA ANIMAL PRODUCCION ANIMAL |
spellingShingle |
SALMO-SALAR L TROUT ONCORHYNCHUS-MYKISS FARMED ATLANTIC SALMON STANDARD SOYBEAN-MEAL PLANT PROTEIN-SOURCES INTESTINAL MICROBIOTA GUT MICROBIOTA BACTERIAL COMMUNITIES NUTRIENT UTILIZATION AQUACULTURE SYSTEMS BIOLOGIA ANIMAL PRODUCCION ANIMAL Estruch, Guillem Collado Amores, María Carmen Peñaranda, D.S. Tomas-Vidal, A. Jover Cerdá, Miguel Pérez-Martínez, G. Martínez-Llorens, Silvia Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene |
topic_facet |
SALMO-SALAR L TROUT ONCORHYNCHUS-MYKISS FARMED ATLANTIC SALMON STANDARD SOYBEAN-MEAL PLANT PROTEIN-SOURCES INTESTINAL MICROBIOTA GUT MICROBIOTA BACTERIAL COMMUNITIES NUTRIENT UTILIZATION AQUACULTURE SYSTEMS BIOLOGIA ANIMAL PRODUCCION ANIMAL |
description |
[EN] Recent studies have demonstrated the impact of diet on microbiota composition, but the essential need for the optimization of production rates and costs forces farms and aquaculture production to carry out continuous dietary tests. In order to understand the effect of total fishmeal replacement by vegetable-based feed in the sea bream (Sparus aurata), the microbial composition of the stomach, foregut, midgut and hindgut was analysed using high-throughput 16S rDNA sequencing, also considering parameters of growth, survival and nutrient utilisation indices.A total of 91,539 16S rRNA filtered-sequences were analysed, with an average number of 3661.56 taxonomically assigned, high-quality sequences per sample. The dominant phyla throughout the whole gastrointestinal tract were Actinobacteria, Protebacteria and Firmicutes. A lower diversity in the stomach in comparison to the other intestinal sections was observed. The microbial composition of the Recirculating Aquaculture System was totally different to that of the sea bream gastrointestinal tract. Total fishmeal replacement had an important impact on microbial profiles but not on diversity. Streptococcus (p-value: 0.043) and Photobacterium (p-value: 0.025) were highly represented in fish fed with fishmeal and vegetable-meal diets, respectively. In the stomach samples with the vegetable diet, reads of chloroplasts and mitochondria from vegetable dietary ingredients were rather abundant. Principal Coordinate Analysis showed a clear differentiation between diets in the microbiota present in the gut, supporting the presence of specific bacterial consortia associated with the diet.Although differences in growth and nutritive parameters were not observed, a negative effect of the vegetable diet on the survival rate was determined. Further studies are required to shed more light on the relationship between the immune system and sea bream gastrointestinal tract microbiota and should consider the modulation of the microbiota to improve the survival rate and nutritive ... |
author2 |
Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal Universitat Politècnica de València |
format |
Article in Journal/Newspaper |
author |
Estruch, Guillem Collado Amores, María Carmen Peñaranda, D.S. Tomas-Vidal, A. Jover Cerdá, Miguel Pérez-Martínez, G. Martínez-Llorens, Silvia |
author_facet |
Estruch, Guillem Collado Amores, María Carmen Peñaranda, D.S. Tomas-Vidal, A. Jover Cerdá, Miguel Pérez-Martínez, G. Martínez-Llorens, Silvia |
author_sort |
Estruch, Guillem |
title |
Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene |
title_short |
Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene |
title_full |
Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene |
title_fullStr |
Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene |
title_full_unstemmed |
Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene |
title_sort |
impact of fishmeal replacement in diets for gilthead sea bream (sparus aurata) on the gastrointestinal microbiota determined by pyrosequencing the 16s rrna gene |
publisher |
Public Library of Science |
publishDate |
2015 |
url |
http://hdl.handle.net/10251/65883 https://doi.org/10.1371/journal.pone.0136389 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
PLoS ONE info:eu-repo/grantAgreement/UPV//SP20120603/ https://dx.doi.org/10.1371/journal.pone.0136389 urn:issn:1932-6203 http://hdl.handle.net/10251/65883 doi:10.1371/journal.pone.0136389 26317431 PMC4552794 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0136389 |
container_title |
PLOS ONE |
container_volume |
10 |
container_issue |
8 |
container_start_page |
e0136389 |
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1766363487473238016 |