Intracellular pH regulation and sperm motility in the European eel
[EN] Sperm activation involves ion fluxes as well as a previous maturation in the seminal plasma, something which has not been studied in depth in marine fish species. pH and potassium are probably involved in sperm maturation and motility in the European eel, as indicated in previous studies. In th...
Published in: | Theriogenology |
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Format: | Article in Journal/Newspaper |
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Elsevier
2020
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Online Access: | http://hdl.handle.net/10251/171696 https://doi.org/10.1016/j.theriogenology.2020.01.026 |
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Politechnical University of Valencia: RiuNet |
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topic |
Anguilla anguilla Sperm maturation Sperm quality Lons Intracellular potassium PRODUCCION ANIMAL |
spellingShingle |
Anguilla anguilla Sperm maturation Sperm quality Lons Intracellular potassium PRODUCCION ANIMAL Pérez Igualada, Luz María Gallego Albiach, Victor Asturiano, Juan F. Intracellular pH regulation and sperm motility in the European eel |
topic_facet |
Anguilla anguilla Sperm maturation Sperm quality Lons Intracellular potassium PRODUCCION ANIMAL |
description |
[EN] Sperm activation involves ion fluxes as well as a previous maturation in the seminal plasma, something which has not been studied in depth in marine fish species. pH and potassium are probably involved in sperm maturation and motility in the European eel, as indicated in previous studies. In this work, the absolute intracellular concentration of potassium in European eel sperm has been determined for the first time. In addition, the intracellular pH (pH(i)) of quiescent eel spermatozoa was determined by two methods (nigericin and null point) that gave similar results, 7.4-7.6. The natural pH(i) range of sperm samples in the quiescent stage was 7.4-8.0, with no evident relationship with sperm motility. However, a linear correlation was seen between sperm motility and the pH of the diluent or extracellular pH (pH(e)), as well as between the pH(i) and the pH of the diluent. The change post-activation in seawater (ASW) depended on the initial pH(e) of the diluent medium. Activation with ASW induced an internal alkalinization of the cells when the sample had previously been diluted in a pH(e) 8.0; an acidification when pH(e) > 8, and no pH(i) variation when pH(e) was 8.0. These experiments indicated that a careful selection of the diluents should be performed before measuring natural pH(i) changes in sperm cells. Thus, studies on the specific seminal plasma composition of marine fish species are necessary before studying their physiology. Furthermore, our study indicates that intracellular alkalinization is not a universal fact during sperm activation. (C) 2020 Elsevier Inc. All rights reserved. Funded by the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 642893 (IMPRESS). Victor Gallego has a postdoc grant from the MICIU (Juan de la Cierva-Incorporacion; IJCI-2017-34200 Pérez Igualada, LM.; Gallego Albiach, V.; Asturiano, JF. (2020). Intracellular pH regulation and sperm motility in the European eel. Theriogenology. 145:48-58. ... |
author2 |
Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal European Commission AGENCIA ESTATAL DE INVESTIGACION |
format |
Article in Journal/Newspaper |
author |
Pérez Igualada, Luz María Gallego Albiach, Victor Asturiano, Juan F. |
author_facet |
Pérez Igualada, Luz María Gallego Albiach, Victor Asturiano, Juan F. |
author_sort |
Pérez Igualada, Luz María |
title |
Intracellular pH regulation and sperm motility in the European eel |
title_short |
Intracellular pH regulation and sperm motility in the European eel |
title_full |
Intracellular pH regulation and sperm motility in the European eel |
title_fullStr |
Intracellular pH regulation and sperm motility in the European eel |
title_full_unstemmed |
Intracellular pH regulation and sperm motility in the European eel |
title_sort |
intracellular ph regulation and sperm motility in the european eel |
publisher |
Elsevier |
publishDate |
2020 |
url |
http://hdl.handle.net/10251/171696 https://doi.org/10.1016/j.theriogenology.2020.01.026 |
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ENVELOPE(-60.873,-60.873,-64.156,-64.156) |
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Cierva |
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Cierva |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
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ftunivpvalencia:oai:riunet.upv.es:10251/171696 2023-05-15T13:28:20+02:00 Intracellular pH regulation and sperm motility in the European eel Pérez Igualada, Luz María Gallego Albiach, Victor Asturiano, Juan F. Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal European Commission AGENCIA ESTATAL DE INVESTIGACION 2020-03-15 http://hdl.handle.net/10251/171696 https://doi.org/10.1016/j.theriogenology.2020.01.026 eng eng Elsevier Theriogenology info:eu-repo/grantAgreement/EC/H2020/642893/EU/Improved production strategies for endangered freshwater species./ info:eu-repo/grantAgreement/AEI//IJCI-2017-34200/ https://doi.org/10.1016/j.theriogenology.2020.01.026 10.1016/j.bbrc.2014.05.100 10.1002/(SICI)1097-010X(19970201)277:2 171::AID-JEZ9>3.0.CO;2-M 10.1002/cm.970250206 10.1111/j.1444-2906.2004.00871.x 10.1016/j.aquaculture.2013.10.022 10.1016/j.cbpa.2016.09.024 10.1002/(SICI)1097-0320(19970401)27:4 374::AID-CYTO9>3.0.CO;2-C 10.1111/j.1095-8649.2000.tb02227.x 10.1016/j.theriogenology.2013.01.019 10.1016/j.aquaculture.2016.04.021 10.1007/s10695-005-1553-x 10.1021/bi00578a012 10.1016/S0990-7440(03)00054-8 10.1023/B:FISH.0000030522.71779.47 10.7554/eLife.07624 10.1111/j.1439-0531.2008.01206.x 10.1007/BF00001167 10.1051/alr:1993008 10.1016/j.aquaculture.2013.05.040 10.1002/cm.10119 10.1023/B:FISH.0000035896.84954.f6 10.1016/0012-1606(83)90004-0 10.1073/pnas.80.5.1327 10.1016/S0021-9258(18)48135-5 urn:issn:0093-691X http://hdl.handle.net/10251/171696 doi:10.1016/j.theriogenology.2020.01.026 31986301 http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess CC-BY-NC-ND Anguilla anguilla Sperm maturation Sperm quality Lons Intracellular potassium PRODUCCION ANIMAL info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2020 ftunivpvalencia https://doi.org/10.1016/j.theriogenology.2020.01.026 2022-06-12T20:57:20Z [EN] Sperm activation involves ion fluxes as well as a previous maturation in the seminal plasma, something which has not been studied in depth in marine fish species. pH and potassium are probably involved in sperm maturation and motility in the European eel, as indicated in previous studies. In this work, the absolute intracellular concentration of potassium in European eel sperm has been determined for the first time. In addition, the intracellular pH (pH(i)) of quiescent eel spermatozoa was determined by two methods (nigericin and null point) that gave similar results, 7.4-7.6. The natural pH(i) range of sperm samples in the quiescent stage was 7.4-8.0, with no evident relationship with sperm motility. However, a linear correlation was seen between sperm motility and the pH of the diluent or extracellular pH (pH(e)), as well as between the pH(i) and the pH of the diluent. The change post-activation in seawater (ASW) depended on the initial pH(e) of the diluent medium. Activation with ASW induced an internal alkalinization of the cells when the sample had previously been diluted in a pH(e) 8.0; an acidification when pH(e) > 8, and no pH(i) variation when pH(e) was 8.0. These experiments indicated that a careful selection of the diluents should be performed before measuring natural pH(i) changes in sperm cells. Thus, studies on the specific seminal plasma composition of marine fish species are necessary before studying their physiology. Furthermore, our study indicates that intracellular alkalinization is not a universal fact during sperm activation. (C) 2020 Elsevier Inc. All rights reserved. Funded by the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 642893 (IMPRESS). Victor Gallego has a postdoc grant from the MICIU (Juan de la Cierva-Incorporacion; IJCI-2017-34200 Pérez Igualada, LM.; Gallego Albiach, V.; Asturiano, JF. (2020). Intracellular pH regulation and sperm motility in the European eel. Theriogenology. 145:48-58. ... Article in Journal/Newspaper Anguilla anguilla European eel Politechnical University of Valencia: RiuNet Cierva ENVELOPE(-60.873,-60.873,-64.156,-64.156) Theriogenology 145 48 58 |