Identification and stable expression of vitellogenin receptor (VTGR) through vitellogenesis in the European eel
[EN] In teleosts, vitellogenin (Vtg) is a phospholipoglycoprotein synthesized by the liver, released into the blood circulation and incorporated into the oocytes via endocytosis mediated by the Vtg receptor (VTGR) to form the yolk granules. The VTGR is crucial for oocyte growth in egg-laying animals...
Published in: | Animal |
---|---|
Main Authors: | , , , , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Cambridge University Press
2020
|
Subjects: | |
Online Access: | http://hdl.handle.net/10251/165354 https://doi.org/10.1017/S1751731119003355 |
id |
ftunivpvalencia:oai:riunet.upv.es:10251/165354 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Politechnical University of Valencia: RiuNet |
op_collection_id |
ftunivpvalencia |
language |
English |
topic |
Anguilla anguilla Teleost Lipoprotein receptor 8 Oogenesis Reproduction PRODUCCION ANIMAL |
spellingShingle |
Anguilla anguilla Teleost Lipoprotein receptor 8 Oogenesis Reproduction PRODUCCION ANIMAL MORINI, MARINA Lafont, A. G. Maugars, G. Baloche, S. Dufour, S. Asturiano, Juan F. Pérez Igualada, Luz María Identification and stable expression of vitellogenin receptor (VTGR) through vitellogenesis in the European eel |
topic_facet |
Anguilla anguilla Teleost Lipoprotein receptor 8 Oogenesis Reproduction PRODUCCION ANIMAL |
description |
[EN] In teleosts, vitellogenin (Vtg) is a phospholipoglycoprotein synthesized by the liver, released into the blood circulation and incorporated into the oocytes via endocytosis mediated by the Vtg receptor (VTGR) to form the yolk granules. The VTGR is crucial for oocyte growth in egg-laying animals but is also present in non-oviparous vertebrates, such as human. The VTGR belongs to the low-density lipoprotein receptor superfamily (LDLR) and is also named very-low-density lipoprotein receptor (VLDLR). In this study, we identified and phylogenetically positioned the VTGR of a basal teleost, the European eel, Anguilla anguilla. We developed quantitative real-time PCR (qRT-PCR) and investigated the tissue distribution of vtgr transcripts. We compared by qRT-PCR the ovarian expression levels of vtgr in juvenile yellow eels and pre-pubertal silver eels. We also analyzed the regulation of ovarian vtgr expression throughout vitellogenesis in experimentally matured eels. The Vtg plasma level was measured by homologous ELISA experimental maturation. Our in silico search and phylogenetical analysis revealed a single vtgr in the European eel, orthologous to other vertebrate vtgr. The qRT-PCR studies revealed that vtgr is mainly expressed in the ovary and also detected in various other tissues such as brain, pituitary, gill, fat, heart, and testis, suggesting some extra-ovarian functions of VTGR. We showed that vtgr is expressed in ovaries of juvenile yellow eels with no higher expression in pre-pubertal silver eels nor in experimentally matured eels. This suggests that vtgr transcription already occurs during early pre-vitellogenesis of immature eels and is not further activated in vitellogenic oocytes. European eel Vtg plasma level increased throughout experimental maturation in agreement with previous studies. Taken together, these results suggest that vtgr transcript levels may not be a limiting step for the uptake of Vtg by the oocyte in the European eel. M.M., A.G.L. and S.D. were granted with Short Term Scientific ... |
author2 |
Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal European Commission European Cooperation in Science and Technology Universitat Politècnica de València |
format |
Article in Journal/Newspaper |
author |
MORINI, MARINA Lafont, A. G. Maugars, G. Baloche, S. Dufour, S. Asturiano, Juan F. Pérez Igualada, Luz María |
author_facet |
MORINI, MARINA Lafont, A. G. Maugars, G. Baloche, S. Dufour, S. Asturiano, Juan F. Pérez Igualada, Luz María |
author_sort |
MORINI, MARINA |
title |
Identification and stable expression of vitellogenin receptor (VTGR) through vitellogenesis in the European eel |
title_short |
Identification and stable expression of vitellogenin receptor (VTGR) through vitellogenesis in the European eel |
title_full |
Identification and stable expression of vitellogenin receptor (VTGR) through vitellogenesis in the European eel |
title_fullStr |
Identification and stable expression of vitellogenin receptor (VTGR) through vitellogenesis in the European eel |
title_full_unstemmed |
Identification and stable expression of vitellogenin receptor (VTGR) through vitellogenesis in the European eel |
title_sort |
identification and stable expression of vitellogenin receptor (vtgr) through vitellogenesis in the european eel |
publisher |
Cambridge University Press |
publishDate |
2020 |
url |
http://hdl.handle.net/10251/165354 https://doi.org/10.1017/S1751731119003355 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
Animal info:eu-repo/grantAgreement/COST//FA1205/EU/Assessing and improving the quality of aquatic animal gametes to enhance aquatic resources - The need to harmonize and standardize evolving methodologies, and improve transfer from academia to industry/ https://doi.org/10.1017/S1751731119003355 10.1093/bioinformatics/bti263 10.1186/1471-2350-13-80 10.1002/mrd.22881 10.1007/BF00174046 10.1002/j.1460-2075.1994.tb06847.x 10.1016/0016-6480(91)90049-C 10.1016/j.ympev.2013.09.002 10.1016/j.ygcen.2010.05.011 10.1016/j.ygcen.2014.12.019 10.1007/978-4-431-65907-5_25 10.1016/0016-6480(88)90090-1 10.1371/journal.pone.0032231 10.1016/j.gene.2012.09.064 10.1146/annurev.biochem.71.110601.135342 10.1007/s10695-012-9612-6 10.1016/j.ygcen.2015.01.025 10.1194/jlr.M300014-JLR200 10.1016/j.ygcen.2019.02.018 10.1016/j.ygcen.2015.11.021 10.1016/j.aquaculture.2014.07.020 10.1016/j.cbpb.2013.07.005 10.1016/j.cbpa.2017.06.021 10.1016/j.cbpa.2015.05.028 10.1016/j.cbpa.2017.02.009 10.1242/jcs.212522 10.1006/bbrc.1996.1040 10.1007/s10695-010-9397-4 10.1111/j.1095-8649.1982.tb03994.x 10.1371/journal.pone.0048931 10.1111/j.1095-8649.2000.tb02227.x 10.1095/biolreprod58.5.1146 10.1194/jlr.M050286 10.1093/bioinformatics/btu033 10.1073/pnas.89.19.9252 10.1093/nar/22.22.4673 10.1016/S0092-8674(00)80782-5 10.1095/biolreprod57.5.1238 10.1016/j.ygcen.2004.12.019 urn:issn:1751-7311 http://hdl.handle.net/10251/165354 doi:10.1017/S1751731119003355 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1017/S1751731119003355 |
container_title |
Animal |
container_volume |
14 |
container_issue |
6 |
container_start_page |
1213 |
op_container_end_page |
1222 |
_version_ |
1766398824205516800 |
spelling |
ftunivpvalencia:oai:riunet.upv.es:10251/165354 2023-05-15T13:27:30+02:00 Identification and stable expression of vitellogenin receptor (VTGR) through vitellogenesis in the European eel MORINI, MARINA Lafont, A. G. Maugars, G. Baloche, S. Dufour, S. Asturiano, Juan F. Pérez Igualada, Luz María Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal European Commission European Cooperation in Science and Technology Universitat Politècnica de València 2020-06 http://hdl.handle.net/10251/165354 https://doi.org/10.1017/S1751731119003355 eng eng Cambridge University Press Animal info:eu-repo/grantAgreement/COST//FA1205/EU/Assessing and improving the quality of aquatic animal gametes to enhance aquatic resources - The need to harmonize and standardize evolving methodologies, and improve transfer from academia to industry/ https://doi.org/10.1017/S1751731119003355 10.1093/bioinformatics/bti263 10.1186/1471-2350-13-80 10.1002/mrd.22881 10.1007/BF00174046 10.1002/j.1460-2075.1994.tb06847.x 10.1016/0016-6480(91)90049-C 10.1016/j.ympev.2013.09.002 10.1016/j.ygcen.2010.05.011 10.1016/j.ygcen.2014.12.019 10.1007/978-4-431-65907-5_25 10.1016/0016-6480(88)90090-1 10.1371/journal.pone.0032231 10.1016/j.gene.2012.09.064 10.1146/annurev.biochem.71.110601.135342 10.1007/s10695-012-9612-6 10.1016/j.ygcen.2015.01.025 10.1194/jlr.M300014-JLR200 10.1016/j.ygcen.2019.02.018 10.1016/j.ygcen.2015.11.021 10.1016/j.aquaculture.2014.07.020 10.1016/j.cbpb.2013.07.005 10.1016/j.cbpa.2017.06.021 10.1016/j.cbpa.2015.05.028 10.1016/j.cbpa.2017.02.009 10.1242/jcs.212522 10.1006/bbrc.1996.1040 10.1007/s10695-010-9397-4 10.1111/j.1095-8649.1982.tb03994.x 10.1371/journal.pone.0048931 10.1111/j.1095-8649.2000.tb02227.x 10.1095/biolreprod58.5.1146 10.1194/jlr.M050286 10.1093/bioinformatics/btu033 10.1073/pnas.89.19.9252 10.1093/nar/22.22.4673 10.1016/S0092-8674(00)80782-5 10.1095/biolreprod57.5.1238 10.1016/j.ygcen.2004.12.019 urn:issn:1751-7311 http://hdl.handle.net/10251/165354 doi:10.1017/S1751731119003355 http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess CC-BY-NC-ND Anguilla anguilla Teleost Lipoprotein receptor 8 Oogenesis Reproduction PRODUCCION ANIMAL info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2020 ftunivpvalencia https://doi.org/10.1017/S1751731119003355 2022-06-12T20:56:04Z [EN] In teleosts, vitellogenin (Vtg) is a phospholipoglycoprotein synthesized by the liver, released into the blood circulation and incorporated into the oocytes via endocytosis mediated by the Vtg receptor (VTGR) to form the yolk granules. The VTGR is crucial for oocyte growth in egg-laying animals but is also present in non-oviparous vertebrates, such as human. The VTGR belongs to the low-density lipoprotein receptor superfamily (LDLR) and is also named very-low-density lipoprotein receptor (VLDLR). In this study, we identified and phylogenetically positioned the VTGR of a basal teleost, the European eel, Anguilla anguilla. We developed quantitative real-time PCR (qRT-PCR) and investigated the tissue distribution of vtgr transcripts. We compared by qRT-PCR the ovarian expression levels of vtgr in juvenile yellow eels and pre-pubertal silver eels. We also analyzed the regulation of ovarian vtgr expression throughout vitellogenesis in experimentally matured eels. The Vtg plasma level was measured by homologous ELISA experimental maturation. Our in silico search and phylogenetical analysis revealed a single vtgr in the European eel, orthologous to other vertebrate vtgr. The qRT-PCR studies revealed that vtgr is mainly expressed in the ovary and also detected in various other tissues such as brain, pituitary, gill, fat, heart, and testis, suggesting some extra-ovarian functions of VTGR. We showed that vtgr is expressed in ovaries of juvenile yellow eels with no higher expression in pre-pubertal silver eels nor in experimentally matured eels. This suggests that vtgr transcription already occurs during early pre-vitellogenesis of immature eels and is not further activated in vitellogenic oocytes. European eel Vtg plasma level increased throughout experimental maturation in agreement with previous studies. Taken together, these results suggest that vtgr transcript levels may not be a limiting step for the uptake of Vtg by the oocyte in the European eel. M.M., A.G.L. and S.D. were granted with Short Term Scientific ... Article in Journal/Newspaper Anguilla anguilla Politechnical University of Valencia: RiuNet Animal 14 6 1213 1222 |