Prevalence and diversity of the Streptobacillus rat-bite fever agent, in three invasive, commensal Rattus species from South Africa
Rat-bite fever is an over-looked, global zoonotic disease that has a mortality rate of up to 13%, if untreated. Historically, this rat-borne disease has been attributed to one of two causative agents, Streptobacillus moniliformis or Spirillum minus. Given the confirmed presence of multiple invasive...
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ftunivpretoria:oai:repository.up.ac.za:2263/88569 2024-05-12T08:10:20+00:00 Prevalence and diversity of the Streptobacillus rat-bite fever agent, in three invasive, commensal Rattus species from South Africa Julius, Rolanda Sunayé Brettschneider, Helene Chimimba, Christian Timothy Bastos, Armanda 2021-06 application/pdf https://repository.up.ac.za/handle/2263/88569 en eng Yale Journal of Biology and Medicine Inc. Julius, R.S., Brettschneider, H., Chimimba, C.T. 2021, 'Prevalence and diversity of the Streptobacillus rat-bite fever agent, in three invasive, commensal Rattus species from South Africa', Yale Journal of Biology and Medicine, vol. 94, no. 2, pp. 217-226. 1551-4056 https://repository.up.ac.za/handle/2263/88569 © 2021, Yale Journal of Biology and Medicine Phylogeny Rattus norvegicus Rattus rattus Rattus tanezumi Streptobacillus moniliformis Streptobacillus notomytis Article 2021 ftunivpretoria 2024-04-17T14:07:13Z Rat-bite fever is an over-looked, global zoonotic disease that has a mortality rate of up to 13%, if untreated. Historically, this rat-borne disease has been attributed to one of two causative agents, Streptobacillus moniliformis or Spirillum minus. Given the confirmed presence of multiple invasive Rattus host species, high rat densities in urban, informal human settlements and increasing reports of rat bites in South Africa, we undertook a retrospective assessment of Streptobacillus in rats sampled from 16 urban sites, in Gauteng, the smallest but most populous Province in South Africa. Using a multi-gene PCR-sequencing approach, we confirmed Streptobacillus presence in 50.9% of oral swabs from three rat species and the presence of two Streptobacillus species, viz. S. moniliformis and S. notomytis. The two members of the cryptic Rattus rattus species complex (R. rattus and R. tanezumi), which are morphologically indistinguishable from each other, had markedly different colonization rates. Whereas 48.6% of rats from this species complex were Streptobacillus-positive, only 32.3% of Rattus tanezumi were positive compared to 61.5% R. rattus. Rattus norvegicus had an intermediate prevalence of 55.6%. Phylogenetic analysis of four gene regions (16S rRNA, gyrB, groEL, recA) identified two discrete lineages; S. moniliformis occurred exclusively in R. norvegicus, and S. notomytis was restricted to the two members of the R. rattus species complex; this represents the first report of Streptobacillus in R. tanezumi. These results highlight a largely overlooked zoonotic threat posed by invasive rats and confirm the presence of two discrete and potentially hostspecific Streptobacillus lineages in South Africa. NRF RISP grant and the DST-NRF Centre of Excellence for Invasion Biology (CIB). https://medicine.yale.edu/yjbm am2022 Mammal Research Institute Zoology and Entomology Article in Journal/Newspaper Rattus rattus University of Pretoria: UPSpace |
institution |
Open Polar |
collection |
University of Pretoria: UPSpace |
op_collection_id |
ftunivpretoria |
language |
English |
topic |
Phylogeny Rattus norvegicus Rattus rattus Rattus tanezumi Streptobacillus moniliformis Streptobacillus notomytis |
spellingShingle |
Phylogeny Rattus norvegicus Rattus rattus Rattus tanezumi Streptobacillus moniliformis Streptobacillus notomytis Julius, Rolanda Sunayé Brettschneider, Helene Chimimba, Christian Timothy Bastos, Armanda Prevalence and diversity of the Streptobacillus rat-bite fever agent, in three invasive, commensal Rattus species from South Africa |
topic_facet |
Phylogeny Rattus norvegicus Rattus rattus Rattus tanezumi Streptobacillus moniliformis Streptobacillus notomytis |
description |
Rat-bite fever is an over-looked, global zoonotic disease that has a mortality rate of up to 13%, if untreated. Historically, this rat-borne disease has been attributed to one of two causative agents, Streptobacillus moniliformis or Spirillum minus. Given the confirmed presence of multiple invasive Rattus host species, high rat densities in urban, informal human settlements and increasing reports of rat bites in South Africa, we undertook a retrospective assessment of Streptobacillus in rats sampled from 16 urban sites, in Gauteng, the smallest but most populous Province in South Africa. Using a multi-gene PCR-sequencing approach, we confirmed Streptobacillus presence in 50.9% of oral swabs from three rat species and the presence of two Streptobacillus species, viz. S. moniliformis and S. notomytis. The two members of the cryptic Rattus rattus species complex (R. rattus and R. tanezumi), which are morphologically indistinguishable from each other, had markedly different colonization rates. Whereas 48.6% of rats from this species complex were Streptobacillus-positive, only 32.3% of Rattus tanezumi were positive compared to 61.5% R. rattus. Rattus norvegicus had an intermediate prevalence of 55.6%. Phylogenetic analysis of four gene regions (16S rRNA, gyrB, groEL, recA) identified two discrete lineages; S. moniliformis occurred exclusively in R. norvegicus, and S. notomytis was restricted to the two members of the R. rattus species complex; this represents the first report of Streptobacillus in R. tanezumi. These results highlight a largely overlooked zoonotic threat posed by invasive rats and confirm the presence of two discrete and potentially hostspecific Streptobacillus lineages in South Africa. NRF RISP grant and the DST-NRF Centre of Excellence for Invasion Biology (CIB). https://medicine.yale.edu/yjbm am2022 Mammal Research Institute Zoology and Entomology |
format |
Article in Journal/Newspaper |
author |
Julius, Rolanda Sunayé Brettschneider, Helene Chimimba, Christian Timothy Bastos, Armanda |
author_facet |
Julius, Rolanda Sunayé Brettschneider, Helene Chimimba, Christian Timothy Bastos, Armanda |
author_sort |
Julius, Rolanda Sunayé |
title |
Prevalence and diversity of the Streptobacillus rat-bite fever agent, in three invasive, commensal Rattus species from South Africa |
title_short |
Prevalence and diversity of the Streptobacillus rat-bite fever agent, in three invasive, commensal Rattus species from South Africa |
title_full |
Prevalence and diversity of the Streptobacillus rat-bite fever agent, in three invasive, commensal Rattus species from South Africa |
title_fullStr |
Prevalence and diversity of the Streptobacillus rat-bite fever agent, in three invasive, commensal Rattus species from South Africa |
title_full_unstemmed |
Prevalence and diversity of the Streptobacillus rat-bite fever agent, in three invasive, commensal Rattus species from South Africa |
title_sort |
prevalence and diversity of the streptobacillus rat-bite fever agent, in three invasive, commensal rattus species from south africa |
publisher |
Yale Journal of Biology and Medicine Inc. |
publishDate |
2021 |
url |
https://repository.up.ac.za/handle/2263/88569 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
Julius, R.S., Brettschneider, H., Chimimba, C.T. 2021, 'Prevalence and diversity of the Streptobacillus rat-bite fever agent, in three invasive, commensal Rattus species from South Africa', Yale Journal of Biology and Medicine, vol. 94, no. 2, pp. 217-226. 1551-4056 https://repository.up.ac.za/handle/2263/88569 |
op_rights |
© 2021, Yale Journal of Biology and Medicine |
_version_ |
1798853772361859072 |