Depth-dependent variables shape community structure and functionality in the Prince Edward Islands

• Published in: Microbial Ecology
• Main Authors: Phoma, Boitumelo Sandra, Makhalanyane, Thulani P.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Springer 2021
• Subjects: Prince Edward Islands (PEIs); Bacteria; Archaea; Community structure; Extracellular enzymatic activity; Correlation analyses; Antarctic; Antarc*; Prince Edward Islands; South African National Antarctic Programme
• Online Access: https://repository.up.ac.za/handle/2263/86287

Physicochemical variables limit and control the distribution of microbial communities in all environments. In the oceans, this may significantly influence functional processes such the consumption of dissolved organic material and nutrient sequestration. Yet, the relative contributions of physical factors, such as water mass variability and depth, on functional processes are underexplored. We assessed microbial community structure and functionality in the Prince Edward Islands (PEIs) using 16S rRNA gene amplicon analysis and extracellular enzymatic activity assays, respectively. We found that depth and nutrients substantially drive the structural patterns of bacteria and archaea in this region. Shifts from epipelagic to bathypelagic zones were linked to decreases in the activities of several extracellular enzymes. These extracellular enzymatic activities were positively correlated with several phyla including several Alphaproteobacteria (including members of the SAR 11 clade and order Rhodospirillales) and Cyanobacteria. We show that depth-dependent variables may be essential drivers of community structure and functionality in the PEIs. The South African National Antarctic Programme (SANAP 110717) of the National Research Foundation (NRF) and the University of Pretoria. http://link.springer.com/journal/248 hj2022 Biochemistry Genetics Microbiology and Plant Pathology