Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts
Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating micr...
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Online Access: | http://hdl.handle.net/2263/57076 https://doi.org/10.1093/gbe/evw189 |
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ftunivpretoria:oai:repository.up.ac.za:2263/57076 2023-05-15T14:02:52+02:00 Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts Thi Le, Phuong Makhalanyane, Thulani P. Guerrero, Leandro D. Vikram, Surendra Van de Peer, Yves Cowan, Don A. 2016 http://hdl.handle.net/2263/57076 https://doi.org/10.1093/gbe/evw189 en eng Oxford University Press http://hdl.handle.net/2263/57076 Thi Le, P, Makhalanyane, TP, Guerrero, LD, Vikram, S, Van de Peer, Y & Cowan, DA 2016, 'Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts', Genome Biology and Evolution, vol. 8, no. 9, pp. 2737-2747. 1759-6653 doi:10.1093/gbe/evw189 The Author(s) 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License. CC-BY-NC Stress response Deserts Comparative metagenomics Antarctica Namib Desert Hypoliths Soils Biomes Article 2016 ftunivpretoria https://doi.org/10.1093/gbe/evw189 2022-05-31T13:25:46Z Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating microbial responses to environmental stressors. However, relatively little is known about the genetic diversity underlying such functional processes in climatically extreme desert systems. This study presents the first comparative metagenome analysis of cyanobacteria-dominated hypolithic communities in hot (Namib Desert, Namibia) and cold (Miers Valley, Antarctica) hyperarid deserts. The most abundant phyla in both hypolith metagenomes were Actinobacteria, Proteobacteria, Cyanobacteria and Bacteroidetes with Cyanobacteria dominating in Antarctic hypoliths. However, no significant differences between the twometagenomeswere identified. The Antarctic hypolithicmetagenome displayed a high number of sequences assigned to sigma factors, replication,recombination andrepair, translation, ribosomal structure,andbiogenesis. In contrast, theNamibDesert metagenome showed a high abundance of sequences assigned to carbohydrate transport and metabolism. Metagenome data analysis also revealed significantdivergence inthe geneticdeterminantsof aminoacidandnucleotidemetabolismbetween these two metagenomes and those of soil from other polar deserts, hot deserts, and non-desert soils. Our results suggest extensive niche differentiation in hypolithic microbial communities from these two extreme environments and a high genetic capacity for survival under environmental extremes. The authors gratefully acknowledge the following organizations: The University of Pretoria Genomics Research Institute (PTL, TPM, DAC, and YVdP), South African National Antarctic Program (SANAP) and the National Research Foundation (NRF) for funding (LG, SV). http://gbe.oxfordjournals.org am2016 Genetics Article in Journal/Newspaper Antarc* Antarctic Antarctica University of Pretoria: UPSpace Antarctic The Antarctic Miers ENVELOPE(163.850,163.850,-78.100,-78.100) Miers Valley ENVELOPE(164.200,164.200,-78.100,-78.100) Genome Biology and Evolution 8 9 2737 2747 |
institution |
Open Polar |
collection |
University of Pretoria: UPSpace |
op_collection_id |
ftunivpretoria |
language |
English |
topic |
Stress response Deserts Comparative metagenomics Antarctica Namib Desert Hypoliths Soils Biomes |
spellingShingle |
Stress response Deserts Comparative metagenomics Antarctica Namib Desert Hypoliths Soils Biomes Thi Le, Phuong Makhalanyane, Thulani P. Guerrero, Leandro D. Vikram, Surendra Van de Peer, Yves Cowan, Don A. Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
topic_facet |
Stress response Deserts Comparative metagenomics Antarctica Namib Desert Hypoliths Soils Biomes |
description |
Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating microbial responses to environmental stressors. However, relatively little is known about the genetic diversity underlying such functional processes in climatically extreme desert systems. This study presents the first comparative metagenome analysis of cyanobacteria-dominated hypolithic communities in hot (Namib Desert, Namibia) and cold (Miers Valley, Antarctica) hyperarid deserts. The most abundant phyla in both hypolith metagenomes were Actinobacteria, Proteobacteria, Cyanobacteria and Bacteroidetes with Cyanobacteria dominating in Antarctic hypoliths. However, no significant differences between the twometagenomeswere identified. The Antarctic hypolithicmetagenome displayed a high number of sequences assigned to sigma factors, replication,recombination andrepair, translation, ribosomal structure,andbiogenesis. In contrast, theNamibDesert metagenome showed a high abundance of sequences assigned to carbohydrate transport and metabolism. Metagenome data analysis also revealed significantdivergence inthe geneticdeterminantsof aminoacidandnucleotidemetabolismbetween these two metagenomes and those of soil from other polar deserts, hot deserts, and non-desert soils. Our results suggest extensive niche differentiation in hypolithic microbial communities from these two extreme environments and a high genetic capacity for survival under environmental extremes. The authors gratefully acknowledge the following organizations: The University of Pretoria Genomics Research Institute (PTL, TPM, DAC, and YVdP), South African National Antarctic Program (SANAP) and the National Research Foundation (NRF) for funding (LG, SV). http://gbe.oxfordjournals.org am2016 Genetics |
format |
Article in Journal/Newspaper |
author |
Thi Le, Phuong Makhalanyane, Thulani P. Guerrero, Leandro D. Vikram, Surendra Van de Peer, Yves Cowan, Don A. |
author_facet |
Thi Le, Phuong Makhalanyane, Thulani P. Guerrero, Leandro D. Vikram, Surendra Van de Peer, Yves Cowan, Don A. |
author_sort |
Thi Le, Phuong |
title |
Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
title_short |
Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
title_full |
Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
title_fullStr |
Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
title_full_unstemmed |
Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
title_sort |
comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
publisher |
Oxford University Press |
publishDate |
2016 |
url |
http://hdl.handle.net/2263/57076 https://doi.org/10.1093/gbe/evw189 |
long_lat |
ENVELOPE(163.850,163.850,-78.100,-78.100) ENVELOPE(164.200,164.200,-78.100,-78.100) |
geographic |
Antarctic The Antarctic Miers Miers Valley |
geographic_facet |
Antarctic The Antarctic Miers Miers Valley |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://hdl.handle.net/2263/57076 Thi Le, P, Makhalanyane, TP, Guerrero, LD, Vikram, S, Van de Peer, Y & Cowan, DA 2016, 'Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts', Genome Biology and Evolution, vol. 8, no. 9, pp. 2737-2747. 1759-6653 doi:10.1093/gbe/evw189 |
op_rights |
The Author(s) 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License. |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.1093/gbe/evw189 |
container_title |
Genome Biology and Evolution |
container_volume |
8 |
container_issue |
9 |
container_start_page |
2737 |
op_container_end_page |
2747 |
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1766273270194110464 |