Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica
Here we provide the first exploration of microbial diversity from three distinct Victoria Valley edaphic habitats, namely lithobionts (hypoliths, endoliths) and surface soils. Using a combination of terminal restriction fragment length polymorphism (T-RFLP) analysis and 16S rRNA gene amplicon pyrose...
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ftunivpretoria:oai:repository.up.ac.za:2263/55751 2023-05-15T13:46:27+02:00 Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica Van Goethem, Marc W. Makhalanyane, Thulani P. Valverde, Angel Cary, Stephen Craig Cowan, Don A. 2016-07-13T09:54:15Z http://hdl.handle.net/2263/55751 https://doi.org/10.1093/femsec/fiw051 en eng Oxford University Press http://hdl.handle.net/2263/55751 0168-6496 (print) 1574-6941 (online) doi:10.1093/femsec/fiw051 © FEMS 2016. All rights reserved. This is a pre-copy-editing, author-produced PDF of an article accepted for publication in FEMS Microbiology Ecology following peer review. The definitive publisher-authenticated version is : Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica, FEMS Microbiology Ecology, vol. 92, no. 4, 2016. doi : 10.1093/femsec/fiw051, is available online at : http://femsec.oxfordjournals.org. Antarctica Bacteria Cyanobacteria Endolith Hypolith Soil Postprint Article 2016 ftunivpretoria https://doi.org/10.1093/femsec/fiw051 2022-05-31T10:48:44Z Here we provide the first exploration of microbial diversity from three distinct Victoria Valley edaphic habitats, namely lithobionts (hypoliths, endoliths) and surface soils. Using a combination of terminal restriction fragment length polymorphism (T-RFLP) analysis and 16S rRNA gene amplicon pyrosequencing we assess community structure and diversity patterns, respectively. Our analysis revealed that habitat type (endolithic versus hypolithic versus surface soils) significantly influenced bacterial community composition, even though dominant phyla such as Actinobacteria (41% of total reads) were common to all samples. Consistent with previous surveys in other Dry Valley ecosystems, we found that lithobionts were colonized by a few highly dominant phylotypes (such as Gemmatimonas and Leptolyngbya). Our analyses also show that soil bacteria were more diverse and evenly distributed than initially expected based on previous evidence. In contrast to total bacteria, the distribution of Cyanobacteria was not strongly influenced by habitat type, although soil- and endolith-specific cyanobacterial lineages were found. The detection of cyanobacterial lineages in these habitats appears to be influenced by the dispersal of aquatic inocula from lacustrine communities or benthic mats which are abundant in Victoria Valley. Together, our results provide insights into the phylogenetic variation and community structure across niche habitats in Victoria Valley. National Research Foundation of South Africa (NRF). University of Pretoria. http://femsec.oxfordjournals.org 2017-04-30 hb2016 Genetics Article in Journal/Newspaper Antarc* Antarctica University of Pretoria: UPSpace Victoria Valley ENVELOPE(162.000,162.000,-77.383,-77.383) FEMS Microbiology Ecology 92 4 fiw051 |
institution |
Open Polar |
collection |
University of Pretoria: UPSpace |
op_collection_id |
ftunivpretoria |
language |
English |
topic |
Antarctica Bacteria Cyanobacteria Endolith Hypolith Soil |
spellingShingle |
Antarctica Bacteria Cyanobacteria Endolith Hypolith Soil Van Goethem, Marc W. Makhalanyane, Thulani P. Valverde, Angel Cary, Stephen Craig Cowan, Don A. Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica |
topic_facet |
Antarctica Bacteria Cyanobacteria Endolith Hypolith Soil |
description |
Here we provide the first exploration of microbial diversity from three distinct Victoria Valley edaphic habitats, namely lithobionts (hypoliths, endoliths) and surface soils. Using a combination of terminal restriction fragment length polymorphism (T-RFLP) analysis and 16S rRNA gene amplicon pyrosequencing we assess community structure and diversity patterns, respectively. Our analysis revealed that habitat type (endolithic versus hypolithic versus surface soils) significantly influenced bacterial community composition, even though dominant phyla such as Actinobacteria (41% of total reads) were common to all samples. Consistent with previous surveys in other Dry Valley ecosystems, we found that lithobionts were colonized by a few highly dominant phylotypes (such as Gemmatimonas and Leptolyngbya). Our analyses also show that soil bacteria were more diverse and evenly distributed than initially expected based on previous evidence. In contrast to total bacteria, the distribution of Cyanobacteria was not strongly influenced by habitat type, although soil- and endolith-specific cyanobacterial lineages were found. The detection of cyanobacterial lineages in these habitats appears to be influenced by the dispersal of aquatic inocula from lacustrine communities or benthic mats which are abundant in Victoria Valley. Together, our results provide insights into the phylogenetic variation and community structure across niche habitats in Victoria Valley. National Research Foundation of South Africa (NRF). University of Pretoria. http://femsec.oxfordjournals.org 2017-04-30 hb2016 Genetics |
format |
Article in Journal/Newspaper |
author |
Van Goethem, Marc W. Makhalanyane, Thulani P. Valverde, Angel Cary, Stephen Craig Cowan, Don A. |
author_facet |
Van Goethem, Marc W. Makhalanyane, Thulani P. Valverde, Angel Cary, Stephen Craig Cowan, Don A. |
author_sort |
Van Goethem, Marc W. |
title |
Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica |
title_short |
Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica |
title_full |
Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica |
title_fullStr |
Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica |
title_full_unstemmed |
Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica |
title_sort |
characterization of bacterial communities in lithobionts and soil niches from victoria valley, antarctica |
publisher |
Oxford University Press |
publishDate |
2016 |
url |
http://hdl.handle.net/2263/55751 https://doi.org/10.1093/femsec/fiw051 |
long_lat |
ENVELOPE(162.000,162.000,-77.383,-77.383) |
geographic |
Victoria Valley |
geographic_facet |
Victoria Valley |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://hdl.handle.net/2263/55751 0168-6496 (print) 1574-6941 (online) doi:10.1093/femsec/fiw051 |
op_rights |
© FEMS 2016. All rights reserved. This is a pre-copy-editing, author-produced PDF of an article accepted for publication in FEMS Microbiology Ecology following peer review. The definitive publisher-authenticated version is : Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica, FEMS Microbiology Ecology, vol. 92, no. 4, 2016. doi : 10.1093/femsec/fiw051, is available online at : http://femsec.oxfordjournals.org. |
op_doi |
https://doi.org/10.1093/femsec/fiw051 |
container_title |
FEMS Microbiology Ecology |
container_volume |
92 |
container_issue |
4 |
container_start_page |
fiw051 |
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1766242868586872832 |