Genetic signatures indicate widespread antibiotic resistance and phage infection in microbial communities of the McMurdo Dry Valleys, East Antarctica
The McMurdo Dry Valleys of Antarctica support extensive yet cryptic microbial communities but little evidence for ‘top-down’ herbivory control. A question therefore arises as to how standing microbial biomass is regulated. Here, we present results from a survey of soil and rock microbial community m...
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ftunivpretoria:oai:repository.up.ac.za:2263/49130 2023-05-15T14:02:19+02:00 Genetic signatures indicate widespread antibiotic resistance and phage infection in microbial communities of the McMurdo Dry Valleys, East Antarctica Wei, Sean T.S. Higgins, Colleen M. Adriaenssens, Evelien M. Cowan, Don A. Pointing, Stephen B. 2015-02 http://hdl.handle.net/2263/49130 https://doi.org/10.1007/s00300-015-1649-4 en eng Springer http://hdl.handle.net/2263/49130 Wei, STS, Higgins, CM, Adriaenssens, EM, Cowan, DA & Pointing, SB 2015, 'Genetic signatures indicate widespread antibiotic resistance and phage infection in microbial communities of the McMurdo Dry Valleys, East Antarctica', Polar Biology, vol. 38, no. 6, pp. 919-925. 0722-4060 (print) 1432-2056 (online) doi:10.1007/s00300-015-1649-4 © Springer-Verlag Berlin Heidelberg 2015.The original publication is available at : http://link.springer.comjournal/300 Antibiotic resistance Antarctic dry valleys Phage Trophic controls Virus Postprint Article 2015 ftunivpretoria https://doi.org/10.1007/s00300-015-1649-4 2022-05-31T13:21:34Z The McMurdo Dry Valleys of Antarctica support extensive yet cryptic microbial communities but little evidence for ‘top-down’ herbivory control. A question therefore arises as to how standing microbial biomass is regulated. Here, we present results from a survey of soil and rock microbial community metagenomes using the GeoChip microarray that demonstrate antibiotic resistance and phage infection are widespread. We interrogated a range of dry valley locations from maritime to extreme inland sites. Antibiotic resistance genes were identified in three categories: beta-lactamases, tetracycline and vanomycin plus a range of transporter genes. Frequency of recovery generally reflected microbial diversity, with greatest abundance among Halobacteria, Proteobacteria and the photosynthetic bacteria (Chlorobi, Chloroflexi and Cyanobacteria). However, no clear differences between locations and soil/rock communities were apparent. Phage signals were also recovered from all locations in soil and rock communities. The Leviviridae, Myoviridae, Podoviridae and Siphoviridae were ubiquitous . The Corticoviridae occurred only in moisturesufficient hyporheic soils, the Microviridae occurred only in maritime and hyporheic sites and an unidentified group within the order Caudovirales occurred only at dry inland sites. We postulate that widespread antibiotic resistance indicates potential inter-specific interaction and that phage signals indicate possible ‘bottom-up’ trophic regulation in the dry valleys. http://link.springer.com/journal/300 2016-02-28 hb2015 Article in Journal/Newspaper Antarc* Antarctic Antarctica East Antarctica McMurdo Dry Valleys Polar Biology University of Pretoria: UPSpace Antarctic East Antarctica McMurdo Dry Valleys Polar Biology 38 6 919 925 |
institution |
Open Polar |
collection |
University of Pretoria: UPSpace |
op_collection_id |
ftunivpretoria |
language |
English |
topic |
Antibiotic resistance Antarctic dry valleys Phage Trophic controls Virus |
spellingShingle |
Antibiotic resistance Antarctic dry valleys Phage Trophic controls Virus Wei, Sean T.S. Higgins, Colleen M. Adriaenssens, Evelien M. Cowan, Don A. Pointing, Stephen B. Genetic signatures indicate widespread antibiotic resistance and phage infection in microbial communities of the McMurdo Dry Valleys, East Antarctica |
topic_facet |
Antibiotic resistance Antarctic dry valleys Phage Trophic controls Virus |
description |
The McMurdo Dry Valleys of Antarctica support extensive yet cryptic microbial communities but little evidence for ‘top-down’ herbivory control. A question therefore arises as to how standing microbial biomass is regulated. Here, we present results from a survey of soil and rock microbial community metagenomes using the GeoChip microarray that demonstrate antibiotic resistance and phage infection are widespread. We interrogated a range of dry valley locations from maritime to extreme inland sites. Antibiotic resistance genes were identified in three categories: beta-lactamases, tetracycline and vanomycin plus a range of transporter genes. Frequency of recovery generally reflected microbial diversity, with greatest abundance among Halobacteria, Proteobacteria and the photosynthetic bacteria (Chlorobi, Chloroflexi and Cyanobacteria). However, no clear differences between locations and soil/rock communities were apparent. Phage signals were also recovered from all locations in soil and rock communities. The Leviviridae, Myoviridae, Podoviridae and Siphoviridae were ubiquitous . The Corticoviridae occurred only in moisturesufficient hyporheic soils, the Microviridae occurred only in maritime and hyporheic sites and an unidentified group within the order Caudovirales occurred only at dry inland sites. We postulate that widespread antibiotic resistance indicates potential inter-specific interaction and that phage signals indicate possible ‘bottom-up’ trophic regulation in the dry valleys. http://link.springer.com/journal/300 2016-02-28 hb2015 |
format |
Article in Journal/Newspaper |
author |
Wei, Sean T.S. Higgins, Colleen M. Adriaenssens, Evelien M. Cowan, Don A. Pointing, Stephen B. |
author_facet |
Wei, Sean T.S. Higgins, Colleen M. Adriaenssens, Evelien M. Cowan, Don A. Pointing, Stephen B. |
author_sort |
Wei, Sean T.S. |
title |
Genetic signatures indicate widespread antibiotic resistance and phage infection in microbial communities of the McMurdo Dry Valleys, East Antarctica |
title_short |
Genetic signatures indicate widespread antibiotic resistance and phage infection in microbial communities of the McMurdo Dry Valleys, East Antarctica |
title_full |
Genetic signatures indicate widespread antibiotic resistance and phage infection in microbial communities of the McMurdo Dry Valleys, East Antarctica |
title_fullStr |
Genetic signatures indicate widespread antibiotic resistance and phage infection in microbial communities of the McMurdo Dry Valleys, East Antarctica |
title_full_unstemmed |
Genetic signatures indicate widespread antibiotic resistance and phage infection in microbial communities of the McMurdo Dry Valleys, East Antarctica |
title_sort |
genetic signatures indicate widespread antibiotic resistance and phage infection in microbial communities of the mcmurdo dry valleys, east antarctica |
publisher |
Springer |
publishDate |
2015 |
url |
http://hdl.handle.net/2263/49130 https://doi.org/10.1007/s00300-015-1649-4 |
geographic |
Antarctic East Antarctica McMurdo Dry Valleys |
geographic_facet |
Antarctic East Antarctica McMurdo Dry Valleys |
genre |
Antarc* Antarctic Antarctica East Antarctica McMurdo Dry Valleys Polar Biology |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica McMurdo Dry Valleys Polar Biology |
op_relation |
http://hdl.handle.net/2263/49130 Wei, STS, Higgins, CM, Adriaenssens, EM, Cowan, DA & Pointing, SB 2015, 'Genetic signatures indicate widespread antibiotic resistance and phage infection in microbial communities of the McMurdo Dry Valleys, East Antarctica', Polar Biology, vol. 38, no. 6, pp. 919-925. 0722-4060 (print) 1432-2056 (online) doi:10.1007/s00300-015-1649-4 |
op_rights |
© Springer-Verlag Berlin Heidelberg 2015.The original publication is available at : http://link.springer.comjournal/300 |
op_doi |
https://doi.org/10.1007/s00300-015-1649-4 |
container_title |
Polar Biology |
container_volume |
38 |
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6 |
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919 |
op_container_end_page |
925 |
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1766272526676131840 |