An alternative method for correcting fluorescence quenching
Under high light intensity, phytoplankton protect their photosystems from bleaching through nonphotochemical quenching processes. The consequence of this is suppression of fluorescence emission, which must be corrected when measuring in situ yield with fluorometers. We present data from the Southern...
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Online Access: | http://hdl.handle.net/2263/45689 https://doi.org/10.5194/os-11-83-2015 |
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ftunivpretoria:oai:repository.up.ac.za:2263/45689 2023-05-15T16:05:45+02:00 An alternative method for correcting fluorescence quenching Biermann, L. Guinet, C. Bester, Marthan Nieuwoudt Brierley, A. Boehme, Lars Hoppema, M. 2015-01-13 http://hdl.handle.net/2263/45689 https://doi.org/10.5194/os-11-83-2015 en eng European Geosciences Union http://hdl.handle.net/2263/45689 Biermann, L, Guinet, C, Bester, M, Brierley, A & Boehme, L 2015, 'An alternative method for correcting fluorescence quenching', Ocean Science, vol. 11, pp. 83-91. 1812-0784 (Print) 1812-0792 (online) doi:10.5194/os-11-83-2015 © Author(s) 2015. CC Attribution 3.0 License. CC-BY Photosystems Fluorescence quenching Fluorescence emission Southern Ocean Article 2015 ftunivpretoria https://doi.org/10.5194/os-11-83-2015 2022-05-31T13:30:28Z Under high light intensity, phytoplankton protect their photosystems from bleaching through nonphotochemical quenching processes. The consequence of this is suppression of fluorescence emission, which must be corrected when measuring in situ yield with fluorometers. We present data from the Southern Ocean, collected over five austral summers by 19 southern elephant seals tagged with fluorometers. Conventionally, fluorescence data collected during the day (quenched) were corrected using the limit of the mixed layer, assuming that phytoplankton are uniformly mixed from the surface to this depth. However, distinct deep fluorescence maxima were measured in approximately 30% of the night (unquenched) data. To account for the evidence that chlorophyll is not uniformly mixed in the upper layer, we propose correcting from the limit of the euphotic zone, defined as the depth at which photosynthetically available radiation is 1%of the surface value. Mixed layer depth exceeded euphotic depth over 80% of the time. Under these conditions, quenching was corrected from the depth of the remotely derived euphotic zone Zeu, and compared with fluorescence corrected from the depth of the density-derived mixed layer. Deep fluorescence maxima were evident in only 10%of the day data when correcting from mixed layer depth. This was doubled to 21% when correcting from Zeu, more closely matching the unquenched (night) data. Furthermore, correcting from Zeu served to conserve non-uniform chlorophyll features found between the 1% light level and mixed layer depth. The MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) and their support is gratefully acknowledged. MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. http://www.ocean-sci.net/11/83/2015/ am2015 Article in Journal/Newspaper Elephant Seals Southern Elephant Seals Southern Ocean University of Pretoria: UPSpace Southern Ocean Austral Ocean Science 11 1 83 91 |
institution |
Open Polar |
collection |
University of Pretoria: UPSpace |
op_collection_id |
ftunivpretoria |
language |
English |
topic |
Photosystems Fluorescence quenching Fluorescence emission Southern Ocean |
spellingShingle |
Photosystems Fluorescence quenching Fluorescence emission Southern Ocean Biermann, L. Guinet, C. Bester, Marthan Nieuwoudt Brierley, A. Boehme, Lars An alternative method for correcting fluorescence quenching |
topic_facet |
Photosystems Fluorescence quenching Fluorescence emission Southern Ocean |
description |
Under high light intensity, phytoplankton protect their photosystems from bleaching through nonphotochemical quenching processes. The consequence of this is suppression of fluorescence emission, which must be corrected when measuring in situ yield with fluorometers. We present data from the Southern Ocean, collected over five austral summers by 19 southern elephant seals tagged with fluorometers. Conventionally, fluorescence data collected during the day (quenched) were corrected using the limit of the mixed layer, assuming that phytoplankton are uniformly mixed from the surface to this depth. However, distinct deep fluorescence maxima were measured in approximately 30% of the night (unquenched) data. To account for the evidence that chlorophyll is not uniformly mixed in the upper layer, we propose correcting from the limit of the euphotic zone, defined as the depth at which photosynthetically available radiation is 1%of the surface value. Mixed layer depth exceeded euphotic depth over 80% of the time. Under these conditions, quenching was corrected from the depth of the remotely derived euphotic zone Zeu, and compared with fluorescence corrected from the depth of the density-derived mixed layer. Deep fluorescence maxima were evident in only 10%of the day data when correcting from mixed layer depth. This was doubled to 21% when correcting from Zeu, more closely matching the unquenched (night) data. Furthermore, correcting from Zeu served to conserve non-uniform chlorophyll features found between the 1% light level and mixed layer depth. The MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) and their support is gratefully acknowledged. MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. http://www.ocean-sci.net/11/83/2015/ am2015 |
author2 |
Hoppema, M. |
format |
Article in Journal/Newspaper |
author |
Biermann, L. Guinet, C. Bester, Marthan Nieuwoudt Brierley, A. Boehme, Lars |
author_facet |
Biermann, L. Guinet, C. Bester, Marthan Nieuwoudt Brierley, A. Boehme, Lars |
author_sort |
Biermann, L. |
title |
An alternative method for correcting fluorescence quenching |
title_short |
An alternative method for correcting fluorescence quenching |
title_full |
An alternative method for correcting fluorescence quenching |
title_fullStr |
An alternative method for correcting fluorescence quenching |
title_full_unstemmed |
An alternative method for correcting fluorescence quenching |
title_sort |
alternative method for correcting fluorescence quenching |
publisher |
European Geosciences Union |
publishDate |
2015 |
url |
http://hdl.handle.net/2263/45689 https://doi.org/10.5194/os-11-83-2015 |
geographic |
Southern Ocean Austral |
geographic_facet |
Southern Ocean Austral |
genre |
Elephant Seals Southern Elephant Seals Southern Ocean |
genre_facet |
Elephant Seals Southern Elephant Seals Southern Ocean |
op_relation |
http://hdl.handle.net/2263/45689 Biermann, L, Guinet, C, Bester, M, Brierley, A & Boehme, L 2015, 'An alternative method for correcting fluorescence quenching', Ocean Science, vol. 11, pp. 83-91. 1812-0784 (Print) 1812-0792 (online) doi:10.5194/os-11-83-2015 |
op_rights |
© Author(s) 2015. CC Attribution 3.0 License. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.5194/os-11-83-2015 |
container_title |
Ocean Science |
container_volume |
11 |
container_issue |
1 |
container_start_page |
83 |
op_container_end_page |
91 |
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1766401639730642944 |