Evidence for successional development in Antarctic hypolithic bacterial communities
Hypoliths (cryptic microbial assemblages that develop on the undersides of translucent rocks) are significant contributors to regional C and N budgets in both hot and cold deserts. Previous studies in the Dry Valleys of Eastern Antarctica have reported three morphologically distinct hypolithic commu...
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ftunivpretoria:oai:repository.up.ac.za:2263/42467 2023-05-15T14:05:19+02:00 Evidence for successional development in Antarctic hypolithic bacterial communities Makhalanyane, Thulani P. Valverde, Angel Cary, Stephen Craig Birkeland, Nils-Kare Tuffin, Marla I. Cowan, Don A. 2013-11 http://hdl.handle.net/2263/42467 https://doi.org/10.1038/ismej.2013.94 en eng Nature Publishing Group http://hdl.handle.net/2263/42467 Makhalanyane, TP, Valverde, A, Birkeland, NK, Cary, SC, Tuffin, IM & Cowan, DA 2013, 'Evidence for successional development in Antarctic hypolithic bacterial communities', ISME Journal, vol. 7, no. 11, pp. 2080-2090. 1751-7362 (print) 1751-7370 (online) doi:10.1038/ismej.2013.94 © 2013 International Society for Microbial Ecology Antarctica Community assembly Hypoliths Pyrosequencing Soil Succession T-RFLP Postprint Article 2013 ftunivpretoria https://doi.org/10.1038/ismej.2013.94 2022-05-31T13:36:47Z Hypoliths (cryptic microbial assemblages that develop on the undersides of translucent rocks) are significant contributors to regional C and N budgets in both hot and cold deserts. Previous studies in the Dry Valleys of Eastern Antarctica have reported three morphologically distinct hypolithic community types: cyanobacteria dominated (type I), fungus dominated (type II) and moss dominated (type III). Here we present terminal-restriction fragment length polymorphism analyses to elucidate the bacterial community structure in hypolithons and the surrounding soils. We show clear and robust distinction in bacterial composition between bulk surface soils and hypolithons. Moreover, the bacterial assemblages were similar in types II and III hypolithons and clearly distinct from those found in type I. Through 16S rRNA gene 454 pyrosequencing, we show that Proteobacteria dominated all three types of hypolithic communities. As expected, Cyanobacteria were more abundant in type I hypolithons, whereas Actinobacteria were relatively more abundant in types II and III hypolithons, and were the dominant group in soils. Using a probabilistic dissimilarity metric and random sampling, we demonstrate that deterministic processes are more important in shaping the structure of the bacterial community found in types II and III hypolithons. Most notably, the data presented in this study suggest that hypolithic bacterial communities establish via a successional model, with the type I hypolithons acting as the basal development state. National Research Foundation (South Africa), the Research Council of Norway (the South Africa Program; grant no. 180352) and the University of the Western Cape. http://www.nature.com/ismej/journal/ hb2014 Article in Journal/Newspaper Antarc* Antarctic Antarctica University of Pretoria: UPSpace Antarctic Norway The ISME Journal 7 11 2080 2090 |
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Open Polar |
collection |
University of Pretoria: UPSpace |
op_collection_id |
ftunivpretoria |
language |
English |
topic |
Antarctica Community assembly Hypoliths Pyrosequencing Soil Succession T-RFLP |
spellingShingle |
Antarctica Community assembly Hypoliths Pyrosequencing Soil Succession T-RFLP Makhalanyane, Thulani P. Valverde, Angel Cary, Stephen Craig Birkeland, Nils-Kare Tuffin, Marla I. Cowan, Don A. Evidence for successional development in Antarctic hypolithic bacterial communities |
topic_facet |
Antarctica Community assembly Hypoliths Pyrosequencing Soil Succession T-RFLP |
description |
Hypoliths (cryptic microbial assemblages that develop on the undersides of translucent rocks) are significant contributors to regional C and N budgets in both hot and cold deserts. Previous studies in the Dry Valleys of Eastern Antarctica have reported three morphologically distinct hypolithic community types: cyanobacteria dominated (type I), fungus dominated (type II) and moss dominated (type III). Here we present terminal-restriction fragment length polymorphism analyses to elucidate the bacterial community structure in hypolithons and the surrounding soils. We show clear and robust distinction in bacterial composition between bulk surface soils and hypolithons. Moreover, the bacterial assemblages were similar in types II and III hypolithons and clearly distinct from those found in type I. Through 16S rRNA gene 454 pyrosequencing, we show that Proteobacteria dominated all three types of hypolithic communities. As expected, Cyanobacteria were more abundant in type I hypolithons, whereas Actinobacteria were relatively more abundant in types II and III hypolithons, and were the dominant group in soils. Using a probabilistic dissimilarity metric and random sampling, we demonstrate that deterministic processes are more important in shaping the structure of the bacterial community found in types II and III hypolithons. Most notably, the data presented in this study suggest that hypolithic bacterial communities establish via a successional model, with the type I hypolithons acting as the basal development state. National Research Foundation (South Africa), the Research Council of Norway (the South Africa Program; grant no. 180352) and the University of the Western Cape. http://www.nature.com/ismej/journal/ hb2014 |
format |
Article in Journal/Newspaper |
author |
Makhalanyane, Thulani P. Valverde, Angel Cary, Stephen Craig Birkeland, Nils-Kare Tuffin, Marla I. Cowan, Don A. |
author_facet |
Makhalanyane, Thulani P. Valverde, Angel Cary, Stephen Craig Birkeland, Nils-Kare Tuffin, Marla I. Cowan, Don A. |
author_sort |
Makhalanyane, Thulani P. |
title |
Evidence for successional development in Antarctic hypolithic bacterial communities |
title_short |
Evidence for successional development in Antarctic hypolithic bacterial communities |
title_full |
Evidence for successional development in Antarctic hypolithic bacterial communities |
title_fullStr |
Evidence for successional development in Antarctic hypolithic bacterial communities |
title_full_unstemmed |
Evidence for successional development in Antarctic hypolithic bacterial communities |
title_sort |
evidence for successional development in antarctic hypolithic bacterial communities |
publisher |
Nature Publishing Group |
publishDate |
2013 |
url |
http://hdl.handle.net/2263/42467 https://doi.org/10.1038/ismej.2013.94 |
geographic |
Antarctic Norway |
geographic_facet |
Antarctic Norway |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://hdl.handle.net/2263/42467 Makhalanyane, TP, Valverde, A, Birkeland, NK, Cary, SC, Tuffin, IM & Cowan, DA 2013, 'Evidence for successional development in Antarctic hypolithic bacterial communities', ISME Journal, vol. 7, no. 11, pp. 2080-2090. 1751-7362 (print) 1751-7370 (online) doi:10.1038/ismej.2013.94 |
op_rights |
© 2013 International Society for Microbial Ecology |
op_doi |
https://doi.org/10.1038/ismej.2013.94 |
container_title |
The ISME Journal |
container_volume |
7 |
container_issue |
11 |
container_start_page |
2080 |
op_container_end_page |
2090 |
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1766277123713007616 |