Microbial response to a mesoscale iron enrichment in the NE Subarctic Pacific: bacterial community composition
Changes in microbial community composition were determined during the subarctic ecosystem response to iron enrichment study (SERIES), a mesoscale Fe enrichment conducted in a high-nutrient low-chlorophyll (HNLC) region of the Northeast Subarctic Pacific, in July 2002. Phylogenetic composition using...
Published in: | Deep Sea Research Part II: Topical Studies in Oceanography |
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Online Access: | https://doi.org/10.1016/j.dsr2.2006.05.040 https://researchportal.port.ac.uk/portal/en/publications/microbial-response-to-a-mesoscale-iron-enrichment-in-the-ne-subarctic-pacific-bacterial-community-composition(5d2ceab0-9e80-41e7-8c7a-0dfb087e7bf7).html |
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ftunivportsmpubl:oai:researchportal.port.ac.uk:publications/5d2ceab0-9e80-41e7-8c7a-0dfb087e7bf7 2023-05-15T18:28:08+02:00 Microbial response to a mesoscale iron enrichment in the NE Subarctic Pacific: bacterial community composition Agawin, N. Hale, Michelle Rivkin, R. Matthews, P. Li, W. 2006 https://doi.org/10.1016/j.dsr2.2006.05.040 https://researchportal.port.ac.uk/portal/en/publications/microbial-response-to-a-mesoscale-iron-enrichment-in-the-ne-subarctic-pacific-bacterial-community-composition(5d2ceab0-9e80-41e7-8c7a-0dfb087e7bf7).html eng eng info:eu-repo/semantics/restrictedAccess Agawin , N , Hale , M , Rivkin , R , Matthews , P & Li , W 2006 , ' Microbial response to a mesoscale iron enrichment in the NE Subarctic Pacific: bacterial community composition ' Deep Sea Research Part II: Topical Studies in Oceanography , vol 53 , no. 20-22 , pp. 2248-2267 . DOI:10.1016/j.dsr2.2006.05.040 /dk/atira/pure/core/subjects/earthsci Earth Sciences article 2006 ftunivportsmpubl https://doi.org/10.1016/j.dsr2.2006.05.040 2017-09-28T19:32:15Z Changes in microbial community composition were determined during the subarctic ecosystem response to iron enrichment study (SERIES), a mesoscale Fe enrichment conducted in a high-nutrient low-chlorophyll (HNLC) region of the Northeast Subarctic Pacific, in July 2002. Phylogenetic composition using fluorescence in situ hybridization (FISH), relative DNA content using flow cytometry (FCM), and cellular morphometrics (shape and volume) of heterotrophic bacteria were used to characterize community composition from samples collected within and below the mixed layer, inside and outside the Fe-patch. The proportion of total cells detected as members of the Cytophaga-Flavobacterium cluster increased in a log-linear manner from 16 (±1.0)% to 47 (±1.9)% in samples within the mixed layer, inside the Fe-enriched patch, while outside the patch, the proportion remained ⩽21 (±2.2)%. Temporal changes in the proportion of cells in the mixed layer with high DNA content (% HDNA) were significantly different inside and outside the Fe-enriched patch, where inside the patch % HDNA increased 2-fold after a week, reaching 93% towards the end of the observation period. Coupling in situ observations with the results of manipulation experiments allowed us to determine the relative contributions of bottom-up (nutrient limitation) and top-down (grazing) processes on heterotrophic bacterial abundance and community composition. Shifts in heterotrophic bacterial community composition inside the Fe-enriched patch were mainly controlled by top-down processes and moderately controlled by bottom-up controls (organic substrate limitation). Article in Journal/Newspaper Subarctic University of Portsmouth: Portsmouth Research Portal Pacific Deep Sea Research Part II: Topical Studies in Oceanography 53 20-22 2248 2267 |
institution |
Open Polar |
collection |
University of Portsmouth: Portsmouth Research Portal |
op_collection_id |
ftunivportsmpubl |
language |
English |
topic |
/dk/atira/pure/core/subjects/earthsci Earth Sciences |
spellingShingle |
/dk/atira/pure/core/subjects/earthsci Earth Sciences Agawin, N. Hale, Michelle Rivkin, R. Matthews, P. Li, W. Microbial response to a mesoscale iron enrichment in the NE Subarctic Pacific: bacterial community composition |
topic_facet |
/dk/atira/pure/core/subjects/earthsci Earth Sciences |
description |
Changes in microbial community composition were determined during the subarctic ecosystem response to iron enrichment study (SERIES), a mesoscale Fe enrichment conducted in a high-nutrient low-chlorophyll (HNLC) region of the Northeast Subarctic Pacific, in July 2002. Phylogenetic composition using fluorescence in situ hybridization (FISH), relative DNA content using flow cytometry (FCM), and cellular morphometrics (shape and volume) of heterotrophic bacteria were used to characterize community composition from samples collected within and below the mixed layer, inside and outside the Fe-patch. The proportion of total cells detected as members of the Cytophaga-Flavobacterium cluster increased in a log-linear manner from 16 (±1.0)% to 47 (±1.9)% in samples within the mixed layer, inside the Fe-enriched patch, while outside the patch, the proportion remained ⩽21 (±2.2)%. Temporal changes in the proportion of cells in the mixed layer with high DNA content (% HDNA) were significantly different inside and outside the Fe-enriched patch, where inside the patch % HDNA increased 2-fold after a week, reaching 93% towards the end of the observation period. Coupling in situ observations with the results of manipulation experiments allowed us to determine the relative contributions of bottom-up (nutrient limitation) and top-down (grazing) processes on heterotrophic bacterial abundance and community composition. Shifts in heterotrophic bacterial community composition inside the Fe-enriched patch were mainly controlled by top-down processes and moderately controlled by bottom-up controls (organic substrate limitation). |
format |
Article in Journal/Newspaper |
author |
Agawin, N. Hale, Michelle Rivkin, R. Matthews, P. Li, W. |
author_facet |
Agawin, N. Hale, Michelle Rivkin, R. Matthews, P. Li, W. |
author_sort |
Agawin, N. |
title |
Microbial response to a mesoscale iron enrichment in the NE Subarctic Pacific: bacterial community composition |
title_short |
Microbial response to a mesoscale iron enrichment in the NE Subarctic Pacific: bacterial community composition |
title_full |
Microbial response to a mesoscale iron enrichment in the NE Subarctic Pacific: bacterial community composition |
title_fullStr |
Microbial response to a mesoscale iron enrichment in the NE Subarctic Pacific: bacterial community composition |
title_full_unstemmed |
Microbial response to a mesoscale iron enrichment in the NE Subarctic Pacific: bacterial community composition |
title_sort |
microbial response to a mesoscale iron enrichment in the ne subarctic pacific: bacterial community composition |
publishDate |
2006 |
url |
https://doi.org/10.1016/j.dsr2.2006.05.040 https://researchportal.port.ac.uk/portal/en/publications/microbial-response-to-a-mesoscale-iron-enrichment-in-the-ne-subarctic-pacific-bacterial-community-composition(5d2ceab0-9e80-41e7-8c7a-0dfb087e7bf7).html |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Agawin , N , Hale , M , Rivkin , R , Matthews , P & Li , W 2006 , ' Microbial response to a mesoscale iron enrichment in the NE Subarctic Pacific: bacterial community composition ' Deep Sea Research Part II: Topical Studies in Oceanography , vol 53 , no. 20-22 , pp. 2248-2267 . DOI:10.1016/j.dsr2.2006.05.040 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.dsr2.2006.05.040 |
container_title |
Deep Sea Research Part II: Topical Studies in Oceanography |
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53 |
container_issue |
20-22 |
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2248 |
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