Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro
The prophenoloxidase (ProPO) system is the origin of melanin production and is considered to be an innate defence mechanism in invertebrates. In different bivalve species, phenoloxidase (PO) is present in the haemolymph as an inactive form of ProPO. The present study focuses on the Pacific adult oys...
Published in: | Fish & Shellfish Immunology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2007
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Online Access: | https://doi.org/10.1016/j.fsi.2006.06.014 https://researchportal.port.ac.uk/portal/en/publications/demonstration-of-a-true-phenoloxidase-activity-and-activation-of-a-propo-cascade-in-pacific-oyster-crassostrea-gigas-thunberg-in-vitro(5640d537-9dbd-4ca9-9331-a1b17b798c9d).html |
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ftunivportsmpubl:oai:researchportal.port.ac.uk:publications/5640d537-9dbd-4ca9-9331-a1b17b798c9d 2023-05-15T15:58:16+02:00 Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro Hellio, Claire Bado-Nilles, A. Gagnaire, B. Renault, T. Thomas-Guyon, H. 2007 https://doi.org/10.1016/j.fsi.2006.06.014 https://researchportal.port.ac.uk/portal/en/publications/demonstration-of-a-true-phenoloxidase-activity-and-activation-of-a-propo-cascade-in-pacific-oyster-crassostrea-gigas-thunberg-in-vitro(5640d537-9dbd-4ca9-9331-a1b17b798c9d).html eng eng info:eu-repo/semantics/restrictedAccess Hellio , C , Bado-Nilles , A , Gagnaire , B , Renault , T & Thomas-Guyon , H 2007 , ' Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro ' Fish & Shellfish Immunology , vol 22 , no. 4 , pp. 433-440 . DOI:10.1016/j.fsi.2006.06.014 /dk/atira/pure/core/subjects/biology Biology article 2007 ftunivportsmpubl https://doi.org/10.1016/j.fsi.2006.06.014 2017-09-28T19:30:22Z The prophenoloxidase (ProPO) system is the origin of melanin production and is considered to be an innate defence mechanism in invertebrates. In different bivalve species, phenoloxidase (PO) is present in the haemolymph as an inactive form of ProPO. The present study focuses on the Pacific adult oyster, Crassostrea gigas, an economically important bivalve species along French coasts. The results indicate that many factors may inhibit the PO-like activity. These include: phenylthiourea (PTU), sodium diethylthiocarbamate (DETC), β-mercaptoethanol and tropolone, which repressed the spontaneous PO activity. The activation of PO-like activity in C. gigas acellular fraction by lipopolysaccharide (LPS) involved participation of other factors, including at least one serine protease. PO was present as proPO in the acellular fraction of haemolymph and haemocytes of C. gigas and could be activated by an exogenous protease (trypsin-N-tosyl-l-phenylalanine chloromethyl ketone) when used at 1 g L−1. Treatment of acellular fractions with other modulators/activators namely LPS (1 g L−1), zymosan (0.6 g L−1) or laminarin (0.6 g L−1) also increased PO-like activity but to a less important way. The study demonstrated the evidence of a true phenoloxidase activity in Pacific oyster, C. gigas (Thunberg). The activation of a proPO system by non-self molecules suggests the role played by PO in vivo in the internal defence mechanisms. Understanding the activation of the ProPO system could enable the evaluation of the health of oyster stocks. Article in Journal/Newspaper Crassostrea gigas Pacific oyster University of Portsmouth: Portsmouth Research Portal Pacific Fish & Shellfish Immunology 22 4 433 440 |
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University of Portsmouth: Portsmouth Research Portal |
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ftunivportsmpubl |
language |
English |
topic |
/dk/atira/pure/core/subjects/biology Biology |
spellingShingle |
/dk/atira/pure/core/subjects/biology Biology Hellio, Claire Bado-Nilles, A. Gagnaire, B. Renault, T. Thomas-Guyon, H. Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro |
topic_facet |
/dk/atira/pure/core/subjects/biology Biology |
description |
The prophenoloxidase (ProPO) system is the origin of melanin production and is considered to be an innate defence mechanism in invertebrates. In different bivalve species, phenoloxidase (PO) is present in the haemolymph as an inactive form of ProPO. The present study focuses on the Pacific adult oyster, Crassostrea gigas, an economically important bivalve species along French coasts. The results indicate that many factors may inhibit the PO-like activity. These include: phenylthiourea (PTU), sodium diethylthiocarbamate (DETC), β-mercaptoethanol and tropolone, which repressed the spontaneous PO activity. The activation of PO-like activity in C. gigas acellular fraction by lipopolysaccharide (LPS) involved participation of other factors, including at least one serine protease. PO was present as proPO in the acellular fraction of haemolymph and haemocytes of C. gigas and could be activated by an exogenous protease (trypsin-N-tosyl-l-phenylalanine chloromethyl ketone) when used at 1 g L−1. Treatment of acellular fractions with other modulators/activators namely LPS (1 g L−1), zymosan (0.6 g L−1) or laminarin (0.6 g L−1) also increased PO-like activity but to a less important way. The study demonstrated the evidence of a true phenoloxidase activity in Pacific oyster, C. gigas (Thunberg). The activation of a proPO system by non-self molecules suggests the role played by PO in vivo in the internal defence mechanisms. Understanding the activation of the ProPO system could enable the evaluation of the health of oyster stocks. |
format |
Article in Journal/Newspaper |
author |
Hellio, Claire Bado-Nilles, A. Gagnaire, B. Renault, T. Thomas-Guyon, H. |
author_facet |
Hellio, Claire Bado-Nilles, A. Gagnaire, B. Renault, T. Thomas-Guyon, H. |
author_sort |
Hellio, Claire |
title |
Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro |
title_short |
Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro |
title_full |
Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro |
title_fullStr |
Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro |
title_full_unstemmed |
Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro |
title_sort |
demonstration of a true phenoloxidase activity and activation of a propo cascade in pacific oyster, crassostrea gigas (thunberg) in vitro |
publishDate |
2007 |
url |
https://doi.org/10.1016/j.fsi.2006.06.014 https://researchportal.port.ac.uk/portal/en/publications/demonstration-of-a-true-phenoloxidase-activity-and-activation-of-a-propo-cascade-in-pacific-oyster-crassostrea-gigas-thunberg-in-vitro(5640d537-9dbd-4ca9-9331-a1b17b798c9d).html |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Hellio , C , Bado-Nilles , A , Gagnaire , B , Renault , T & Thomas-Guyon , H 2007 , ' Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro ' Fish & Shellfish Immunology , vol 22 , no. 4 , pp. 433-440 . DOI:10.1016/j.fsi.2006.06.014 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.fsi.2006.06.014 |
container_title |
Fish & Shellfish Immunology |
container_volume |
22 |
container_issue |
4 |
container_start_page |
433 |
op_container_end_page |
440 |
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1766393989549785088 |