Prevention of urinary catheter-associated infections by coating antimicrobial peptides from crowberry endophytes
Urinary catheters are extensively used in hospitals, being responsible for about 75% of hospital-acquired infections. In this work, a de novo designed antimicrobial peptide (AMP) Chain201D was studied in the context of urinary catheter-associated infections. Chain201D showed excellent antimicrobial...
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ftunivporto:oai:repositorio-aberto.up.pt:10216/136232 2023-06-18T03:40:21+02:00 Prevention of urinary catheter-associated infections by coating antimicrobial peptides from crowberry endophytes Monteiro, C Costa, F Pirttilä, A Tejesvi, M Martins, MCL Instituto de Investigação e Inovação em Saúde 2019 application/pdf https://hdl.handle.net/10216/136232 https://doi.org/10.1038/s41598-019-47108-5 eng eng Nature Publishing Group Scientific Reports, vol.9(1):10753 https://www.nature.com/articles/s41598-019-47108-5 2045-2322 https://hdl.handle.net/10216/136232 doi:10.1038/s41598-019-47108-5 info:eu-repo/semantics/openAccess Anti-Infective Agents / therapeutic use Antimicrobial Cationic Peptides / therapeutic use Catheter-Related Infections / prevention & control Endophytes / chemistry Ericaceae / chemistry Escherichia coli / drug effects Humans Klebsiella pneumoniae / drug effects Microbial Sensitivity Tests Pseudomonas aeruginosa / drug effects Staphylococcus aureus / drug effects Urinary Catheterization / adverse effects Urinary Catheterization / methods Urinary Catheters / adverse effects Urinary Catheters / microbiology info:eu-repo/semantics/article 2019 ftunivporto https://doi.org/10.1038/s41598-019-47108-5 2023-06-06T21:22:19Z Urinary catheters are extensively used in hospitals, being responsible for about 75% of hospital-acquired infections. In this work, a de novo designed antimicrobial peptide (AMP) Chain201D was studied in the context of urinary catheter-associated infections. Chain201D showed excellent antimicrobial activity against relevant ATCC strains and clinical isolates of bacteria and yeast and demonstrated high stability in a wide range of temperatures, pH and salt concentrations. Moreover, the bactericidal activity of Chain201D immobilized on a model surface was studied against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), some of the most prevalent strains found in urinary catheter-associated infections. Chain201D was successfully tethered to ((1-mercapto-11-undecyl)-(tetra(ethylene glycol) (EG4)) terminated self-assembled monolayers (SAMs), (EG4-SAMs), activated by 1,1'-Carbonyldiimidazole (CDI) at different concentrations. Chain201D surfaces can bind and kill by contact a high percentage of adherent bacteria. These achievements are obtained without any peptide modification (for chemoselective conjugation) and without the use of a spacer. Moreover, increased amounts of immobilized AMP lead to higher numbers of adhered/dead bacteria, revealing a concentration-dependent behaviour and demonstrating that Chain201D has excellent potential for developing antimicrobial urinary catheters. AntINFECT: Bioengineered Advanced Therapies for Problematic Infected Wounds POCI-01-0145-FEDER-031781) and Institute for Research and Innovation in Health Sciences (UID/BIM/04293/2019). C. Monteiro would like to acknowledge national funding through FCT – Fundação para a Ciência e a Tecnologia, I.P., provided by the contract-program and according to numbers 4, 5 and 6 of art. 23 of Law no. 57/2016 of 29th August, as amended by Law no. 57/2017 of 19th July. Professor Paula Gomes from Faculdade de Ciências da Universidade do Porto (FC-UP), Porto Peptide Synthesis Facility (POP-UP) for CEM 7 peptide. The funding received ... Article in Journal/Newspaper Crowberry Repositório Aberto da Universidade do Porto Scientific Reports 9 1 |
institution |
Open Polar |
collection |
Repositório Aberto da Universidade do Porto |
op_collection_id |
ftunivporto |
language |
English |
topic |
Anti-Infective Agents / therapeutic use Antimicrobial Cationic Peptides / therapeutic use Catheter-Related Infections / prevention & control Endophytes / chemistry Ericaceae / chemistry Escherichia coli / drug effects Humans Klebsiella pneumoniae / drug effects Microbial Sensitivity Tests Pseudomonas aeruginosa / drug effects Staphylococcus aureus / drug effects Urinary Catheterization / adverse effects Urinary Catheterization / methods Urinary Catheters / adverse effects Urinary Catheters / microbiology |
spellingShingle |
Anti-Infective Agents / therapeutic use Antimicrobial Cationic Peptides / therapeutic use Catheter-Related Infections / prevention & control Endophytes / chemistry Ericaceae / chemistry Escherichia coli / drug effects Humans Klebsiella pneumoniae / drug effects Microbial Sensitivity Tests Pseudomonas aeruginosa / drug effects Staphylococcus aureus / drug effects Urinary Catheterization / adverse effects Urinary Catheterization / methods Urinary Catheters / adverse effects Urinary Catheters / microbiology Monteiro, C Costa, F Pirttilä, A Tejesvi, M Martins, MCL Prevention of urinary catheter-associated infections by coating antimicrobial peptides from crowberry endophytes |
topic_facet |
Anti-Infective Agents / therapeutic use Antimicrobial Cationic Peptides / therapeutic use Catheter-Related Infections / prevention & control Endophytes / chemistry Ericaceae / chemistry Escherichia coli / drug effects Humans Klebsiella pneumoniae / drug effects Microbial Sensitivity Tests Pseudomonas aeruginosa / drug effects Staphylococcus aureus / drug effects Urinary Catheterization / adverse effects Urinary Catheterization / methods Urinary Catheters / adverse effects Urinary Catheters / microbiology |
description |
Urinary catheters are extensively used in hospitals, being responsible for about 75% of hospital-acquired infections. In this work, a de novo designed antimicrobial peptide (AMP) Chain201D was studied in the context of urinary catheter-associated infections. Chain201D showed excellent antimicrobial activity against relevant ATCC strains and clinical isolates of bacteria and yeast and demonstrated high stability in a wide range of temperatures, pH and salt concentrations. Moreover, the bactericidal activity of Chain201D immobilized on a model surface was studied against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), some of the most prevalent strains found in urinary catheter-associated infections. Chain201D was successfully tethered to ((1-mercapto-11-undecyl)-(tetra(ethylene glycol) (EG4)) terminated self-assembled monolayers (SAMs), (EG4-SAMs), activated by 1,1'-Carbonyldiimidazole (CDI) at different concentrations. Chain201D surfaces can bind and kill by contact a high percentage of adherent bacteria. These achievements are obtained without any peptide modification (for chemoselective conjugation) and without the use of a spacer. Moreover, increased amounts of immobilized AMP lead to higher numbers of adhered/dead bacteria, revealing a concentration-dependent behaviour and demonstrating that Chain201D has excellent potential for developing antimicrobial urinary catheters. AntINFECT: Bioengineered Advanced Therapies for Problematic Infected Wounds POCI-01-0145-FEDER-031781) and Institute for Research and Innovation in Health Sciences (UID/BIM/04293/2019). C. Monteiro would like to acknowledge national funding through FCT – Fundação para a Ciência e a Tecnologia, I.P., provided by the contract-program and according to numbers 4, 5 and 6 of art. 23 of Law no. 57/2016 of 29th August, as amended by Law no. 57/2017 of 19th July. Professor Paula Gomes from Faculdade de Ciências da Universidade do Porto (FC-UP), Porto Peptide Synthesis Facility (POP-UP) for CEM 7 peptide. The funding received ... |
author2 |
Instituto de Investigação e Inovação em Saúde |
format |
Article in Journal/Newspaper |
author |
Monteiro, C Costa, F Pirttilä, A Tejesvi, M Martins, MCL |
author_facet |
Monteiro, C Costa, F Pirttilä, A Tejesvi, M Martins, MCL |
author_sort |
Monteiro, C |
title |
Prevention of urinary catheter-associated infections by coating antimicrobial peptides from crowberry endophytes |
title_short |
Prevention of urinary catheter-associated infections by coating antimicrobial peptides from crowberry endophytes |
title_full |
Prevention of urinary catheter-associated infections by coating antimicrobial peptides from crowberry endophytes |
title_fullStr |
Prevention of urinary catheter-associated infections by coating antimicrobial peptides from crowberry endophytes |
title_full_unstemmed |
Prevention of urinary catheter-associated infections by coating antimicrobial peptides from crowberry endophytes |
title_sort |
prevention of urinary catheter-associated infections by coating antimicrobial peptides from crowberry endophytes |
publisher |
Nature Publishing Group |
publishDate |
2019 |
url |
https://hdl.handle.net/10216/136232 https://doi.org/10.1038/s41598-019-47108-5 |
genre |
Crowberry |
genre_facet |
Crowberry |
op_relation |
Scientific Reports, vol.9(1):10753 https://www.nature.com/articles/s41598-019-47108-5 2045-2322 https://hdl.handle.net/10216/136232 doi:10.1038/s41598-019-47108-5 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/s41598-019-47108-5 |
container_title |
Scientific Reports |
container_volume |
9 |
container_issue |
1 |
_version_ |
1769005348369203200 |