Cyanobacteria phylogenetic studies reveal evidence for polyphyletic genera from thermal and freshwater habitats
Cyanobacteria are among the most diverse morphological microorganisms that inhabit a great variety of habitats. Their presence in the Azores, a volcanic archipelago of nine islands in the middle of the North Atlantic Ocean, has already been reported. However, due to the high diversity of cyanobacter...
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ftunivporto:oai:repositorio-aberto.up.pt:10216/130469 2023-06-18T03:42:07+02:00 Cyanobacteria phylogenetic studies reveal evidence for polyphyletic genera from thermal and freshwater habitats Cordeiro, R. Luz, R. Vasconcelos, V. Gonçalves, V. Fonseca, A. Centro Interdisciplinar de Investigação Marinha e Ambiental 2020 application/pdf https://hdl.handle.net/10216/130469 https://doi.org/10.3390/d12080298 eng eng MDPI Diversity 2020, 12(8), 298 https://www.mdpi.com/1424-2818/12/8/298 ISSN 1424-2818 https://hdl.handle.net/10216/130469 https://doi.org/10.3390/d12080298 info:eu-repo/semantics/openAccess 16S rRNA Azores Cyanophyceae Freshwater Oceanic islands Polyphyletic genera Thermal info:eu-repo/semantics/article 2020 ftunivporto https://doi.org/10.3390/d12080298 2023-06-06T20:45:24Z Cyanobacteria are among the most diverse morphological microorganisms that inhabit a great variety of habitats. Their presence in the Azores, a volcanic archipelago of nine islands in the middle of the North Atlantic Ocean, has already been reported. However, due to the high diversity of cyanobacteria habitats, their biodiversity is still understudied, mainly in extreme environments. To address this, a total of 156 cyanobacteria strains from Azores lakes, streams, thermal and terrestrial habitats were isolated. Identification was made based on a polyphasic approach using classical taxonomy (morphological characteristics and environmental data) and phylogeny among 81 strains assessed by maximum likelihood and Bayesian analysis of 16S rDNA partial sequences. The 156 isolates showed a high genera diversity (38) belonging to the orders Chroococcales, Nostocales, Oscillatoriales, and Synechococcales. Eleven new genera for the Azores habitats are here reported, reinforcing that cyanobacteria biodiversity in these islands is still much understudied. Phylogenetic analysis showed 14 clusters associated with these cyanobacteria orders, with evidence for six new genera and valuable information towards Microchaete/Coleospermum taxonomic revision that better reflects species environmental distribution. These results emphasize the need for cyanobacteria taxonomy revisions, through polyphasic studies, mainly in Synechococcales order and in the Microchaete/Coleospermum, Nostoc, and Anabaena genera. Rita Cordeiro was supported by a Ph.D. grant (M3.1.a/F/017/2015) from the Fundo Regional da Ciência e Tecnologia (FRCT). This work was funded by FEDER funds through the Interreg-MAC 2014-2020 Program under the projects REBECA - Red de excelencia en biotecnología azul (algas) de la región macaronesia (MAC1.1a/060) and REBECA-CCT - Red de Excelencia en Biotecnología Azul de la Región Macaronésica. Consolidación, Certificación y Transferencia (MAC2/1.1b/269), and by Portuguese National Funds, through FCT - Fundação para a Ciência e a ... Article in Journal/Newspaper North Atlantic Repositório Aberto da Universidade do Porto Diversity 12 8 298 |
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Open Polar |
collection |
Repositório Aberto da Universidade do Porto |
op_collection_id |
ftunivporto |
language |
English |
topic |
16S rRNA Azores Cyanophyceae Freshwater Oceanic islands Polyphyletic genera Thermal |
spellingShingle |
16S rRNA Azores Cyanophyceae Freshwater Oceanic islands Polyphyletic genera Thermal Cordeiro, R. Luz, R. Vasconcelos, V. Gonçalves, V. Fonseca, A. Cyanobacteria phylogenetic studies reveal evidence for polyphyletic genera from thermal and freshwater habitats |
topic_facet |
16S rRNA Azores Cyanophyceae Freshwater Oceanic islands Polyphyletic genera Thermal |
description |
Cyanobacteria are among the most diverse morphological microorganisms that inhabit a great variety of habitats. Their presence in the Azores, a volcanic archipelago of nine islands in the middle of the North Atlantic Ocean, has already been reported. However, due to the high diversity of cyanobacteria habitats, their biodiversity is still understudied, mainly in extreme environments. To address this, a total of 156 cyanobacteria strains from Azores lakes, streams, thermal and terrestrial habitats were isolated. Identification was made based on a polyphasic approach using classical taxonomy (morphological characteristics and environmental data) and phylogeny among 81 strains assessed by maximum likelihood and Bayesian analysis of 16S rDNA partial sequences. The 156 isolates showed a high genera diversity (38) belonging to the orders Chroococcales, Nostocales, Oscillatoriales, and Synechococcales. Eleven new genera for the Azores habitats are here reported, reinforcing that cyanobacteria biodiversity in these islands is still much understudied. Phylogenetic analysis showed 14 clusters associated with these cyanobacteria orders, with evidence for six new genera and valuable information towards Microchaete/Coleospermum taxonomic revision that better reflects species environmental distribution. These results emphasize the need for cyanobacteria taxonomy revisions, through polyphasic studies, mainly in Synechococcales order and in the Microchaete/Coleospermum, Nostoc, and Anabaena genera. Rita Cordeiro was supported by a Ph.D. grant (M3.1.a/F/017/2015) from the Fundo Regional da Ciência e Tecnologia (FRCT). This work was funded by FEDER funds through the Interreg-MAC 2014-2020 Program under the projects REBECA - Red de excelencia en biotecnología azul (algas) de la región macaronesia (MAC1.1a/060) and REBECA-CCT - Red de Excelencia en Biotecnología Azul de la Región Macaronésica. Consolidación, Certificación y Transferencia (MAC2/1.1b/269), and by Portuguese National Funds, through FCT - Fundação para a Ciência e a ... |
author2 |
Centro Interdisciplinar de Investigação Marinha e Ambiental |
format |
Article in Journal/Newspaper |
author |
Cordeiro, R. Luz, R. Vasconcelos, V. Gonçalves, V. Fonseca, A. |
author_facet |
Cordeiro, R. Luz, R. Vasconcelos, V. Gonçalves, V. Fonseca, A. |
author_sort |
Cordeiro, R. |
title |
Cyanobacteria phylogenetic studies reveal evidence for polyphyletic genera from thermal and freshwater habitats |
title_short |
Cyanobacteria phylogenetic studies reveal evidence for polyphyletic genera from thermal and freshwater habitats |
title_full |
Cyanobacteria phylogenetic studies reveal evidence for polyphyletic genera from thermal and freshwater habitats |
title_fullStr |
Cyanobacteria phylogenetic studies reveal evidence for polyphyletic genera from thermal and freshwater habitats |
title_full_unstemmed |
Cyanobacteria phylogenetic studies reveal evidence for polyphyletic genera from thermal and freshwater habitats |
title_sort |
cyanobacteria phylogenetic studies reveal evidence for polyphyletic genera from thermal and freshwater habitats |
publisher |
MDPI |
publishDate |
2020 |
url |
https://hdl.handle.net/10216/130469 https://doi.org/10.3390/d12080298 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
Diversity 2020, 12(8), 298 https://www.mdpi.com/1424-2818/12/8/298 ISSN 1424-2818 https://hdl.handle.net/10216/130469 https://doi.org/10.3390/d12080298 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/d12080298 |
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Diversity |
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12 |
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8 |
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298 |
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1769007937244626944 |