Diversity of bacterial biosynthetic genes in maritime antarctica
Bacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) are two pharmaceutically important families of NPs and recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) and non-ribosomal peptide synthet...
Published in: | Microorganisms |
---|---|
Main Authors: | , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI
2020
|
Subjects: | |
Online Access: | https://hdl.handle.net/10216/130444 https://doi.org/10.3390/microorganisms8020279 |
id |
ftunivporto:oai:repositorio-aberto.up.pt:10216/130444 |
---|---|
record_format |
openpolar |
spelling |
ftunivporto:oai:repositorio-aberto.up.pt:10216/130444 2023-06-18T03:37:41+02:00 Diversity of bacterial biosynthetic genes in maritime antarctica Rego, A. Sousa, A.G.G. Santos, J.P. Pascoal, F. Canário, J. Leão, P.N. Magalhães, C. Centro Interdisciplinar de Investigação Marinha e Ambiental 2020 application/pdf https://hdl.handle.net/10216/130444 https://doi.org/10.3390/microorganisms8020279 eng eng MDPI Microorganisms 2020, 8(2), 279 https://www.mdpi.com/2076-2607/8/2/279 ISSN 2076-2607 https://hdl.handle.net/10216/130444 https://doi.org/10.3390/microorganisms8020279 info:eu-repo/semantics/openAccess Adenylation (AD) Antarctica Biosynthetic genes Computational bioprospection Ketosynthase (KS) Natural products (NPs) Non-ribosomal peptides (NRPs) Polyketides (PKs) info:eu-repo/semantics/article 2020 ftunivporto https://doi.org/10.3390/microorganisms8020279 2023-06-06T21:15:51Z Bacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) are two pharmaceutically important families of NPs and recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes, likely to be involved in the production of novel metabolites. Despite this, the diversity of secondary metabolites genes in Antarctica is still poorly explored. In this study, a computational bioprospection approach was employed to study the diversity and identity of PKS and NRPS genes to one of the most biodiverse areas in maritime Antarctica—Maxwell Bay. Amplicon sequencing of soil samples targeting ketosynthase (KS) and adenylation (AD) domains of PKS and NRPS genes, respectively, revealed abundant and unexplored chemical diversity in this peninsula. About 20% of AD domain sequences were only distantly related to characterized biosynthetic genes. Several PKS and NRPS genes were found to be closely associated to recently described metabolites including those from uncultured and candidate phyla. The combination of new approaches in computational biology and new culture-dependent and -independent strategies is thus critical for the recovery of the potential novel chemistry encoded in Antarctica microorganisms. Fundação para a Ciência e a Tecnologia (FCT) funded this study through the grant PTDC/CTA- AMB/30997/2017 to CM, the IF research contract IF/01358/2014 to PNL, a PhD scholarship to AR (SFRH/BD/140567/2018) and within the scope of UIDB/04423/2020 and UIDP/04423/2020. The authors acknowledge the Portuguese Polar Program (PROPOLAR) and the Chilean Antarctic Institute (INACH) for providing logistics and traveling financial support during CONTANTARC-3 campaign in Maxwell Bay/King George Island. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Maxwell Bay Repositório Aberto da Universidade do Porto Antarctic Inach ENVELOPE(-60.783,-60.783,-62.467,-62.467) King George Island Maxwell Bay ENVELOPE(-58.859,-58.859,-62.223,-62.223) Microorganisms 8 2 279 |
institution |
Open Polar |
collection |
Repositório Aberto da Universidade do Porto |
op_collection_id |
ftunivporto |
language |
English |
topic |
Adenylation (AD) Antarctica Biosynthetic genes Computational bioprospection Ketosynthase (KS) Natural products (NPs) Non-ribosomal peptides (NRPs) Polyketides (PKs) |
spellingShingle |
Adenylation (AD) Antarctica Biosynthetic genes Computational bioprospection Ketosynthase (KS) Natural products (NPs) Non-ribosomal peptides (NRPs) Polyketides (PKs) Rego, A. Sousa, A.G.G. Santos, J.P. Pascoal, F. Canário, J. Leão, P.N. Magalhães, C. Diversity of bacterial biosynthetic genes in maritime antarctica |
topic_facet |
Adenylation (AD) Antarctica Biosynthetic genes Computational bioprospection Ketosynthase (KS) Natural products (NPs) Non-ribosomal peptides (NRPs) Polyketides (PKs) |
description |
Bacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) are two pharmaceutically important families of NPs and recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes, likely to be involved in the production of novel metabolites. Despite this, the diversity of secondary metabolites genes in Antarctica is still poorly explored. In this study, a computational bioprospection approach was employed to study the diversity and identity of PKS and NRPS genes to one of the most biodiverse areas in maritime Antarctica—Maxwell Bay. Amplicon sequencing of soil samples targeting ketosynthase (KS) and adenylation (AD) domains of PKS and NRPS genes, respectively, revealed abundant and unexplored chemical diversity in this peninsula. About 20% of AD domain sequences were only distantly related to characterized biosynthetic genes. Several PKS and NRPS genes were found to be closely associated to recently described metabolites including those from uncultured and candidate phyla. The combination of new approaches in computational biology and new culture-dependent and -independent strategies is thus critical for the recovery of the potential novel chemistry encoded in Antarctica microorganisms. Fundação para a Ciência e a Tecnologia (FCT) funded this study through the grant PTDC/CTA- AMB/30997/2017 to CM, the IF research contract IF/01358/2014 to PNL, a PhD scholarship to AR (SFRH/BD/140567/2018) and within the scope of UIDB/04423/2020 and UIDP/04423/2020. The authors acknowledge the Portuguese Polar Program (PROPOLAR) and the Chilean Antarctic Institute (INACH) for providing logistics and traveling financial support during CONTANTARC-3 campaign in Maxwell Bay/King George Island. |
author2 |
Centro Interdisciplinar de Investigação Marinha e Ambiental |
format |
Article in Journal/Newspaper |
author |
Rego, A. Sousa, A.G.G. Santos, J.P. Pascoal, F. Canário, J. Leão, P.N. Magalhães, C. |
author_facet |
Rego, A. Sousa, A.G.G. Santos, J.P. Pascoal, F. Canário, J. Leão, P.N. Magalhães, C. |
author_sort |
Rego, A. |
title |
Diversity of bacterial biosynthetic genes in maritime antarctica |
title_short |
Diversity of bacterial biosynthetic genes in maritime antarctica |
title_full |
Diversity of bacterial biosynthetic genes in maritime antarctica |
title_fullStr |
Diversity of bacterial biosynthetic genes in maritime antarctica |
title_full_unstemmed |
Diversity of bacterial biosynthetic genes in maritime antarctica |
title_sort |
diversity of bacterial biosynthetic genes in maritime antarctica |
publisher |
MDPI |
publishDate |
2020 |
url |
https://hdl.handle.net/10216/130444 https://doi.org/10.3390/microorganisms8020279 |
long_lat |
ENVELOPE(-60.783,-60.783,-62.467,-62.467) ENVELOPE(-58.859,-58.859,-62.223,-62.223) |
geographic |
Antarctic Inach King George Island Maxwell Bay |
geographic_facet |
Antarctic Inach King George Island Maxwell Bay |
genre |
Antarc* Antarctic Antarctica King George Island Maxwell Bay |
genre_facet |
Antarc* Antarctic Antarctica King George Island Maxwell Bay |
op_relation |
Microorganisms 2020, 8(2), 279 https://www.mdpi.com/2076-2607/8/2/279 ISSN 2076-2607 https://hdl.handle.net/10216/130444 https://doi.org/10.3390/microorganisms8020279 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/microorganisms8020279 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
2 |
container_start_page |
279 |
_version_ |
1769010460723511296 |