Changes in intestinal microbiota, immune- and stress-related transcript levels in Senegalese sole (Solea senegalensis) fed plant ingredient diets intercropped with probiotics or immunostimulants
Senegalese sole (Solea senegalensis) is a highly valued flatfish that grows well with diets containing plant ingredients but their effects on immune competence is still a matter of debate. The current study aimed to examine changes in innate immune parameters and gut microbiota in Senegalese sole fe...
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Online Access: | https://hdl.handle.net/10216/120279 https://doi.org/10.1016/j.aquaculture.2016.03.002 |
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ftunivporto:oai:repositorio-aberto.up.pt:10216/120279 2023-05-15T17:24:42+02:00 Changes in intestinal microbiota, immune- and stress-related transcript levels in Senegalese sole (Solea senegalensis) fed plant ingredient diets intercropped with probiotics or immunostimulants Batista S. Ozório R.O.A. Kollias S. Dhanasiri A.K. Lokesh J. Kiron V. Valente L.M.P. Fernandes J.M.O. CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental 2016 application/pdf https://hdl.handle.net/10216/120279 https://doi.org/10.1016/j.aquaculture.2016.03.002 eng eng Elsevier Aquaculture, vol. 458, p. 149-157 http://dx.doi.org/10.1016/j.aquaculture.2016.03.002 00448486 https://hdl.handle.net/10216/120279 doi:10.1016/j.aquaculture.2016.03.002 info:eu-repo/semantics/restrictedAccess info:eu-repo/semantics/article 2016 ftunivporto https://doi.org/10.1016/j.aquaculture.2016.03.002 2020-03-01T18:16:56Z Senegalese sole (Solea senegalensis) is a highly valued flatfish that grows well with diets containing plant ingredients but their effects on immune competence is still a matter of debate. The current study aimed to examine changes in innate immune parameters and gut microbiota in Senegalese sole fed with 35% or 72% of plant ingredients with or without probiotic or yeast supplementation. Overall, fish fed diets with 72% of plant ingredients showed lower transcript levels of key immune- and stress-related genes in distal intestine, rectum and head-kidney than the 35% diets. In particular, hsp90b mRNA levels in distal intestine were down-regulated by 70% and 60% with the use of high content of plant ingredients in the diet containing the multispecies probiotic and autolyzed yeast, respectively. Denaturing gradient gel electrophoresis showed lower similarity values for distal intestine than rectum. Also fish fed high content of plant ingredients displayed lower similarity values, pointing to a difference in the microbial populations between fish fed different plant ingredient contents on the diet. Our data revealed that inclusion of plant ingredients was associated with differences in gene expression and a more diverse microbiota profile but without a significant effect on growth performance. Moreover, probiotic supplementation resulted in up-regulation of hsp90b, gpx, cat and apoa1 transcript levels in distal intestine concomitantly with a growth rate reduction compared to non-supplemented fish. Statement of relevance: There is an increasing trend in the aquaculture industry to replace fishmeal for plant ingredients, as a means of promoting sustainability of the industry. This paper contributes significantly to our limited knowledge of how plant ingredients and supplements affect gut microbiota and immunocompetence. © 2016 Elsevier S. M. G. Batista is supported by FCT — SFRH/BD/76668/2011. This work was also supported by the PROBIOSOLEA Project with the financial support of Quadro de Referência Estratégico Nacional — QREN and Programa Operacional Regional do Norte — ON2 (Ref. no. 13551), supported by the European fund for regional development FEDER. We would like to thank to CIIMAR/ICBAS (UP) and FBA (University of Nordland) for the use of the facilities and equipment and for technical support. Article in Journal/Newspaper Nordland Nordland University of Nordland Repositório Aberto da Universidade do Porto Aquaculture 458 149 157 |
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Repositório Aberto da Universidade do Porto |
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English |
description |
Senegalese sole (Solea senegalensis) is a highly valued flatfish that grows well with diets containing plant ingredients but their effects on immune competence is still a matter of debate. The current study aimed to examine changes in innate immune parameters and gut microbiota in Senegalese sole fed with 35% or 72% of plant ingredients with or without probiotic or yeast supplementation. Overall, fish fed diets with 72% of plant ingredients showed lower transcript levels of key immune- and stress-related genes in distal intestine, rectum and head-kidney than the 35% diets. In particular, hsp90b mRNA levels in distal intestine were down-regulated by 70% and 60% with the use of high content of plant ingredients in the diet containing the multispecies probiotic and autolyzed yeast, respectively. Denaturing gradient gel electrophoresis showed lower similarity values for distal intestine than rectum. Also fish fed high content of plant ingredients displayed lower similarity values, pointing to a difference in the microbial populations between fish fed different plant ingredient contents on the diet. Our data revealed that inclusion of plant ingredients was associated with differences in gene expression and a more diverse microbiota profile but without a significant effect on growth performance. Moreover, probiotic supplementation resulted in up-regulation of hsp90b, gpx, cat and apoa1 transcript levels in distal intestine concomitantly with a growth rate reduction compared to non-supplemented fish. Statement of relevance: There is an increasing trend in the aquaculture industry to replace fishmeal for plant ingredients, as a means of promoting sustainability of the industry. This paper contributes significantly to our limited knowledge of how plant ingredients and supplements affect gut microbiota and immunocompetence. © 2016 Elsevier S. M. G. Batista is supported by FCT — SFRH/BD/76668/2011. This work was also supported by the PROBIOSOLEA Project with the financial support of Quadro de Referência Estratégico Nacional — QREN and Programa Operacional Regional do Norte — ON2 (Ref. no. 13551), supported by the European fund for regional development FEDER. We would like to thank to CIIMAR/ICBAS (UP) and FBA (University of Nordland) for the use of the facilities and equipment and for technical support. |
author2 |
CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental |
format |
Article in Journal/Newspaper |
author |
Batista S. Ozório R.O.A. Kollias S. Dhanasiri A.K. Lokesh J. Kiron V. Valente L.M.P. Fernandes J.M.O. |
spellingShingle |
Batista S. Ozório R.O.A. Kollias S. Dhanasiri A.K. Lokesh J. Kiron V. Valente L.M.P. Fernandes J.M.O. Changes in intestinal microbiota, immune- and stress-related transcript levels in Senegalese sole (Solea senegalensis) fed plant ingredient diets intercropped with probiotics or immunostimulants |
author_facet |
Batista S. Ozório R.O.A. Kollias S. Dhanasiri A.K. Lokesh J. Kiron V. Valente L.M.P. Fernandes J.M.O. |
author_sort |
Batista S. |
title |
Changes in intestinal microbiota, immune- and stress-related transcript levels in Senegalese sole (Solea senegalensis) fed plant ingredient diets intercropped with probiotics or immunostimulants |
title_short |
Changes in intestinal microbiota, immune- and stress-related transcript levels in Senegalese sole (Solea senegalensis) fed plant ingredient diets intercropped with probiotics or immunostimulants |
title_full |
Changes in intestinal microbiota, immune- and stress-related transcript levels in Senegalese sole (Solea senegalensis) fed plant ingredient diets intercropped with probiotics or immunostimulants |
title_fullStr |
Changes in intestinal microbiota, immune- and stress-related transcript levels in Senegalese sole (Solea senegalensis) fed plant ingredient diets intercropped with probiotics or immunostimulants |
title_full_unstemmed |
Changes in intestinal microbiota, immune- and stress-related transcript levels in Senegalese sole (Solea senegalensis) fed plant ingredient diets intercropped with probiotics or immunostimulants |
title_sort |
changes in intestinal microbiota, immune- and stress-related transcript levels in senegalese sole (solea senegalensis) fed plant ingredient diets intercropped with probiotics or immunostimulants |
publisher |
Elsevier |
publishDate |
2016 |
url |
https://hdl.handle.net/10216/120279 https://doi.org/10.1016/j.aquaculture.2016.03.002 |
genre |
Nordland Nordland University of Nordland |
genre_facet |
Nordland Nordland University of Nordland |
op_relation |
Aquaculture, vol. 458, p. 149-157 http://dx.doi.org/10.1016/j.aquaculture.2016.03.002 00448486 https://hdl.handle.net/10216/120279 doi:10.1016/j.aquaculture.2016.03.002 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.aquaculture.2016.03.002 |
container_title |
Aquaculture |
container_volume |
458 |
container_start_page |
149 |
op_container_end_page |
157 |
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1766115820112445440 |