Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares
We report the first mountain hare (Lepus timidus) transcriptome, produced by de novo assembly of RNA-sequencing reads. Data were obtained from eight specimens sampled in two localities, Alps and Ireland. The mountain hare tends to be replaced by the invading European hare (Lepus europaeus) in their...
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ftunivporto:oai:repositorio-aberto.up.pt:10216/110951 2023-06-18T03:41:39+02:00 Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares Marques, João P. Ferreira, Mafalda, S. Farelo, Liliana Callahan, Colin M. Hacklander, Klaus Jenny, Hannes Montgomery, W. Ian Reid, Neil Good, Jeffrey M. Alves, Paulo C. Melo-Ferreira, José Faculdade de Ciências 2017-12-05 application/pdf https://hdl.handle.net/10216/110951 https://doi.org/10.1038/sdata.2017.178 eng eng https://hdl.handle.net/10216/110951 doi:10.1038/sdata.2017.178 P-00N-A30 info:eu-repo/semantics/openAccess info:eu-repo/semantics/article 2017 ftunivporto https://doi.org/10.1038/sdata.2017.178 2023-06-06T21:25:00Z We report the first mountain hare (Lepus timidus) transcriptome, produced by de novo assembly of RNA-sequencing reads. Data were obtained from eight specimens sampled in two localities, Alps and Ireland. The mountain hare tends to be replaced by the invading European hare (Lepus europaeus) in their numerous contact zones where the species hybridize, which affects their gene pool to a yet unquantified degree. We characterize and annotate the mountain hare transcriptome, detect polymorphism in the two analysed populations and use previously published data on the European hare (three specimens, representing the European lineage of the species) to identify 4 672 putative diagnostic sites between the species. A subset of 85 random independent SNPs was successfully validated using PCR and Sanger sequencing. These valuable genomic resources can be used to design tools to assess population status and monitor hybridization between species. Article in Journal/Newspaper Lepus timidus mountain hare Repositório Aberto da Universidade do Porto Scientific Data 4 1 |
institution |
Open Polar |
collection |
Repositório Aberto da Universidade do Porto |
op_collection_id |
ftunivporto |
language |
English |
description |
We report the first mountain hare (Lepus timidus) transcriptome, produced by de novo assembly of RNA-sequencing reads. Data were obtained from eight specimens sampled in two localities, Alps and Ireland. The mountain hare tends to be replaced by the invading European hare (Lepus europaeus) in their numerous contact zones where the species hybridize, which affects their gene pool to a yet unquantified degree. We characterize and annotate the mountain hare transcriptome, detect polymorphism in the two analysed populations and use previously published data on the European hare (three specimens, representing the European lineage of the species) to identify 4 672 putative diagnostic sites between the species. A subset of 85 random independent SNPs was successfully validated using PCR and Sanger sequencing. These valuable genomic resources can be used to design tools to assess population status and monitor hybridization between species. |
author2 |
Faculdade de Ciências |
format |
Article in Journal/Newspaper |
author |
Marques, João P. Ferreira, Mafalda, S. Farelo, Liliana Callahan, Colin M. Hacklander, Klaus Jenny, Hannes Montgomery, W. Ian Reid, Neil Good, Jeffrey M. Alves, Paulo C. Melo-Ferreira, José |
spellingShingle |
Marques, João P. Ferreira, Mafalda, S. Farelo, Liliana Callahan, Colin M. Hacklander, Klaus Jenny, Hannes Montgomery, W. Ian Reid, Neil Good, Jeffrey M. Alves, Paulo C. Melo-Ferreira, José Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares |
author_facet |
Marques, João P. Ferreira, Mafalda, S. Farelo, Liliana Callahan, Colin M. Hacklander, Klaus Jenny, Hannes Montgomery, W. Ian Reid, Neil Good, Jeffrey M. Alves, Paulo C. Melo-Ferreira, José |
author_sort |
Marques, João P. |
title |
Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares |
title_short |
Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares |
title_full |
Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares |
title_fullStr |
Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares |
title_full_unstemmed |
Data Descriptor: Mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with European hares |
title_sort |
data descriptor: mountain hare transcriptome and diagnostic markers as resources to monitor hybridization with european hares |
publishDate |
2017 |
url |
https://hdl.handle.net/10216/110951 https://doi.org/10.1038/sdata.2017.178 |
genre |
Lepus timidus mountain hare |
genre_facet |
Lepus timidus mountain hare |
op_relation |
https://hdl.handle.net/10216/110951 doi:10.1038/sdata.2017.178 P-00N-A30 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/sdata.2017.178 |
container_title |
Scientific Data |
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4 |
container_issue |
1 |
_version_ |
1769007290402209792 |