Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus -related and V. aestuarianus strains from bacterial cultures

International audience To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be...

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Bibliographic Details
Published in:Journal of Microbiological Methods
Main Authors: Saulnier, Denis, de Decker, Sophie, Tourbiez, Delphine, Travers, Marie-Agnes
Other Authors: Ecosystèmes Insulaires Océaniens (UMR 241) (EIO), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Polynésie Française (UPF)-Institut Louis Malardé Papeete (ILM), Institut de Recherche pour le Développement (IRD), Unité Santé, Génétique et Microbiologie des Mollusques (SGMM), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2017
Subjects:
[SDV]Life Sciences [q-bio]
Pacific
Crassostrea gigas
Online Access:https://hal.science/hal-04201905
https://doi.org/10.1016/j.mimet.2017.07.002
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Summary:International audience To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and specific and will be particularly valuable for epidemiologic studies.

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