Ciguatoxins activate the Calcineurin signalling pathway in Yeasts: Potential for development of an alternative detection tool?
International audience Ciguatoxins (CTXs) are lipid-soluble polyether compounds produced by dinoflagellates from the genus Gambierdiscus spp. typically found in tropical and subtropical zones. This endemic area is however rapidly expanding due to environmental perturbations, and both toxic Gambierdi...
Published in: | Environmental Research |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2018
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Subjects: | |
Online Access: | https://hal.science/hal-01680541 https://doi.org/10.1016/j.envres.2017.12.022 |
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ftunivpolynesief:oai:HAL:hal-01680541v1 |
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openpolar |
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Open Polar |
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Université de la Polynésie française (upf): HAL |
op_collection_id |
ftunivpolynesief |
language |
English |
topic |
detection man food poisoning calcineurin pathway yeasts ciguatoxins bioassays humain intoxication alimentaire produit de la mer canal à sodium cytotoxicité [SDV.BIO]Life Sciences [q-bio]/Biotechnology |
spellingShingle |
detection man food poisoning calcineurin pathway yeasts ciguatoxins bioassays humain intoxication alimentaire produit de la mer canal à sodium cytotoxicité [SDV.BIO]Life Sciences [q-bio]/Biotechnology Martin-Yken, Helene Gironde, Camille Derick, Sylvain Darius, Hélène Taiana Furger, Christophe Laurent, Dominique Chinain, Mireille Ciguatoxins activate the Calcineurin signalling pathway in Yeasts: Potential for development of an alternative detection tool? |
topic_facet |
detection man food poisoning calcineurin pathway yeasts ciguatoxins bioassays humain intoxication alimentaire produit de la mer canal à sodium cytotoxicité [SDV.BIO]Life Sciences [q-bio]/Biotechnology |
description |
International audience Ciguatoxins (CTXs) are lipid-soluble polyether compounds produced by dinoflagellates from the genus Gambierdiscus spp. typically found in tropical and subtropical zones. This endemic area is however rapidly expanding due to environmental perturbations, and both toxic Gambierdiscus spp. and ciguatoxic fishes have been recently identified in the North Atlantic Ocean (Madeira and Canary islands) and Mediterranean Sea. Ciguatoxins bind to Voltage Gated Sodium Channels on the membranes of sensory neurons, causing Ciguatera Fish Poisoning (CFP) in humans, a disease characterized by a complex array of gastrointestinal, neurological, neuropsychological, and cardiovascular symptoms. Although CFP is the most frequently reported non bacterial food-borne poisoning worldwide, there is still no simple and quick way of detecting CTXs in contaminated samples. In the prospect to engineer rapid and easy-to-use CTXs live cells-based tests, we have studied the effects of CTXs on the yeast Saccharomyces cerevisiae, a unicellular model which displays a remarkable conservation of cellular signalling pathways with higher eukaryotes. Taking advantage of this high level of conservation, yeast strains have been genetically modified to encode specific transcriptional reporters responding to CTXs exposure. These yeast strains were further exposed to different concentrations of either purified CTX or micro-algal extracts containing CTXs. Our data establish that CTXs are not cytotoxic to yeast cells even at concentrations as high as 1μM, and cause an increase in the level of free intracellular calcium in yeast cells. Concomitantly, a dose-dependent activation of the calcineurin signalling pathway is observed, as assessed by measuring the activity of specific transcriptional reporters in the engineered yeast strains. These findings offer promising prospects regarding the potential development of a yeast cells-based test that could supplement or, in some instances, replace current methods for the routine detection of ... |
author2 |
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP) Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS) Équipe Micro et nanosystèmes HyperFréquences Fluidiques (LAAS-MH2F) Laboratoire d'analyse et d'architecture des systèmes (LAAS) Université Toulouse Capitole (UT Capitole) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J) Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole) Université de Toulouse (UT) LED Engineering Development Institut Louis Malardé Papeete (ILM) Institut de Recherche pour le Développement (IRD) Ecosystèmes Insulaires Océaniens (UMR 241) (EIO) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Polynésie Française (UPF)-Institut Louis Malardé Papeete (ILM) Pharmacochimie et Biologie pour le Développement (PHARMA-DEV) Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT) Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Institut de Recherche pour le Développement (IRD Polynésie ) |
format |
Article in Journal/Newspaper |
author |
Martin-Yken, Helene Gironde, Camille Derick, Sylvain Darius, Hélène Taiana Furger, Christophe Laurent, Dominique Chinain, Mireille |
author_facet |
Martin-Yken, Helene Gironde, Camille Derick, Sylvain Darius, Hélène Taiana Furger, Christophe Laurent, Dominique Chinain, Mireille |
author_sort |
Martin-Yken, Helene |
title |
Ciguatoxins activate the Calcineurin signalling pathway in Yeasts: Potential for development of an alternative detection tool? |
title_short |
Ciguatoxins activate the Calcineurin signalling pathway in Yeasts: Potential for development of an alternative detection tool? |
title_full |
Ciguatoxins activate the Calcineurin signalling pathway in Yeasts: Potential for development of an alternative detection tool? |
title_fullStr |
Ciguatoxins activate the Calcineurin signalling pathway in Yeasts: Potential for development of an alternative detection tool? |
title_full_unstemmed |
Ciguatoxins activate the Calcineurin signalling pathway in Yeasts: Potential for development of an alternative detection tool? |
title_sort |
ciguatoxins activate the calcineurin signalling pathway in yeasts: potential for development of an alternative detection tool? |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.science/hal-01680541 https://doi.org/10.1016/j.envres.2017.12.022 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
ISSN: 0013-9351 EISSN: 1096-0953 Environmental Research https://hal.science/hal-01680541 Environmental Research, 2018, 162, pp.144-151. ⟨10.1016/j.envres.2017.12.022⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envres.2017.12.022 info:eu-repo/semantics/altIdentifier/pmid/29306662 hal-01680541 https://hal.science/hal-01680541 doi:10.1016/j.envres.2017.12.022 PRODINRA: 417250 PUBMED: 29306662 WOS: 000426325300017 |
op_doi |
https://doi.org/10.1016/j.envres.2017.12.022 |
container_title |
Environmental Research |
container_volume |
162 |
container_start_page |
144 |
op_container_end_page |
151 |
_version_ |
1810464479623774208 |
spelling |
ftunivpolynesief:oai:HAL:hal-01680541v1 2024-09-15T18:24:10+00:00 Ciguatoxins activate the Calcineurin signalling pathway in Yeasts: Potential for development of an alternative detection tool? Martin-Yken, Helene Gironde, Camille Derick, Sylvain Darius, Hélène Taiana Furger, Christophe Laurent, Dominique Chinain, Mireille Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP) Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS) Équipe Micro et nanosystèmes HyperFréquences Fluidiques (LAAS-MH2F) Laboratoire d'analyse et d'architecture des systèmes (LAAS) Université Toulouse Capitole (UT Capitole) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J) Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole) Université de Toulouse (UT) LED Engineering Development Institut Louis Malardé Papeete (ILM) Institut de Recherche pour le Développement (IRD) Ecosystèmes Insulaires Océaniens (UMR 241) (EIO) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Polynésie Française (UPF)-Institut Louis Malardé Papeete (ILM) Pharmacochimie et Biologie pour le Développement (PHARMA-DEV) Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT) Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Institut de Recherche pour le Développement (IRD Polynésie ) 2018-04 https://hal.science/hal-01680541 https://doi.org/10.1016/j.envres.2017.12.022 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envres.2017.12.022 info:eu-repo/semantics/altIdentifier/pmid/29306662 hal-01680541 https://hal.science/hal-01680541 doi:10.1016/j.envres.2017.12.022 PRODINRA: 417250 PUBMED: 29306662 WOS: 000426325300017 ISSN: 0013-9351 EISSN: 1096-0953 Environmental Research https://hal.science/hal-01680541 Environmental Research, 2018, 162, pp.144-151. ⟨10.1016/j.envres.2017.12.022⟩ detection man food poisoning calcineurin pathway yeasts ciguatoxins bioassays humain intoxication alimentaire produit de la mer canal à sodium cytotoxicité [SDV.BIO]Life Sciences [q-bio]/Biotechnology info:eu-repo/semantics/article Journal articles 2018 ftunivpolynesief https://doi.org/10.1016/j.envres.2017.12.022 2024-07-22T14:02:32Z International audience Ciguatoxins (CTXs) are lipid-soluble polyether compounds produced by dinoflagellates from the genus Gambierdiscus spp. typically found in tropical and subtropical zones. This endemic area is however rapidly expanding due to environmental perturbations, and both toxic Gambierdiscus spp. and ciguatoxic fishes have been recently identified in the North Atlantic Ocean (Madeira and Canary islands) and Mediterranean Sea. Ciguatoxins bind to Voltage Gated Sodium Channels on the membranes of sensory neurons, causing Ciguatera Fish Poisoning (CFP) in humans, a disease characterized by a complex array of gastrointestinal, neurological, neuropsychological, and cardiovascular symptoms. Although CFP is the most frequently reported non bacterial food-borne poisoning worldwide, there is still no simple and quick way of detecting CTXs in contaminated samples. In the prospect to engineer rapid and easy-to-use CTXs live cells-based tests, we have studied the effects of CTXs on the yeast Saccharomyces cerevisiae, a unicellular model which displays a remarkable conservation of cellular signalling pathways with higher eukaryotes. Taking advantage of this high level of conservation, yeast strains have been genetically modified to encode specific transcriptional reporters responding to CTXs exposure. These yeast strains were further exposed to different concentrations of either purified CTX or micro-algal extracts containing CTXs. Our data establish that CTXs are not cytotoxic to yeast cells even at concentrations as high as 1μM, and cause an increase in the level of free intracellular calcium in yeast cells. Concomitantly, a dose-dependent activation of the calcineurin signalling pathway is observed, as assessed by measuring the activity of specific transcriptional reporters in the engineered yeast strains. These findings offer promising prospects regarding the potential development of a yeast cells-based test that could supplement or, in some instances, replace current methods for the routine detection of ... Article in Journal/Newspaper North Atlantic Université de la Polynésie française (upf): HAL Environmental Research 162 144 151 |